Human TNFRSF17 / BCMA (CD269) Protein (ECD, His Tag) 10620-H08H from Sino Biological, Inc.

Product Specs
ItemHuman TNFRSF17 / BCMA (CD269) Protein (ECD, His Tag)
CompanySino Biological, Inc.
Price $1,088.00Supplier Page View Company Product Page
Catalog Number10620-H08H
Size500 µg
Conjugate/TagC-His
FormatLyophilized from sterile PBS, pH 7.4
Purity≥ 95 % as determined by SDS-PAGE. ≥ 95 % as determined by SEC-HPLC
Activity1.Immobilized Human BAFF (Cat:10056-H01H) at 2µg/mL (100 µl/well) can bind Human BCMA (Cat:10620-H08H), The EC50 of Human BCMA (Cat:10620-H08H) is 30-80 ng/mL(Routinely tested). 2.Immobilized Mouse TNFSF13(Cat:50965-M01H) at 2µg/mL (100 µl/well) can bind Human BCMA (Cat:10620-H08H), The EC50 of Human BCMA (Cat:10620-H08H) is 0.6-2.1 ng/mL(QC tested). 3.293 cells were transduced with anti-BCMA-scFv and subjected to flow cytometric analysis using recombinant human BCMA (left, Cat. No. 10620-H08H, red) and a negative control protein (blue). The cells were then stained with a PE-conjugated anti-His antibody. Non-transduced 293 cells were used as a control (right)(Routinely tested). 4.Loaded Recombinant Human BAFF / BLyS / TNFSF13B Protein, hFc Tag (Cat. No. 10056-H01H) on ProA Biosensor, can bind Recombinant Human BCMA Protein, His Tag (Cat. No. 10620-H08H) with an affinity constant of 0.267 µM as determined in BLI assay (Sartorius Octet RED384) (Routinely tested). 5.Immobilized Anti-BCMA Antibody, Human IgG1 at 2 µg/mL (100 µL/well) can bind Recombinant Human TNFRSF17 / BCMA (CD269) Protein (ECD, His Tag) (Cat: 10620-H08H), the EC50 is 3-10 ng/mL (Routinely tested). 6.Anti-BCMA Antibody, Human IgG1 captured on Protein A chip can bind Recombinant Human BCMA Protein, His Tag (Cat. No. 10620-H08H) with an affinity constant of 22.3 nM as determined in an SPR assay (Biacore 8K) (Routinely tested). 7.Loaded Biotinylated Recombinant Human BAFF/BLyS/TNFSF13B Protein (His & AVI Tag) (Cat. No. 10056-H56H1-B) on SA Biosensor, can bind Recombinant Human BCMA Protein (ECD, His Tag) (Cat. No. 10620-H08H ) with an affinity constant of 0.853 µM as determined in BLI assay (Sartorius Octet RED384) (Routinely tested). 8.Immobilized Recombinant Human APRIL/TNFSF13 Protein (Fc Tag) (Cat: 10610-H01H2) at 5 µg/mL (100 µL/well) can bind Recombinant Human TNFRSF17 / BCMA (CD269) Protein (ECD, His Tag) (Cat: 10620-H08H), the EC50 is 2.6 ng/mL(Routinely tested). 9.Immobilized Recombinant Human TNFRSF17 / BCMA (CD269) Protein (ECD, His Tag) (Cat: 10620-H08H) at 2 µg/mL (100 µL/well) can bind Anti-Human BCMA Antibody, the EC50 is 1.7 ng/mL(Routinely tested)
TypeRecombinant protein
Molecular WeightThe recombinant human TNFRSF17 consists of 65 amino acids and predicts a molecular mass of 7.3 kDa. As a result of glycosylation, It migrates as an approximately 16.9 and 12.4 KDa band in SDS-PAGE under reducing conditions
SpeciesHuman
Molecule NameTNFRSF17
Source/Expression SystemHEK293 Cells
NCBI Full Gene NameTNF receptor superfamily member 17
NCBI Gene AliasesBCM, BCMA, CD269, TNFRSF13A