Description
Description: Recombinant human DOT1L (KMT4) (1-435) was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag.
Scientific Background: DOT1-like protein (DOT1L) specifically methylates lysine-79 of histone H3 in the globular domain. DOT1L lacks a SET-domain, which is commonly considered to mediate lysine methylation of histones (1, 2). DOT1 homologs exist in a variety of eukaryotic organisms. The activity of this methyltransferase is involved in cell cycle regulation and leukemogenesis (3)