Lys-C, Mass Spec Grade VA1170 from Promega

Product Specs
ItemLys-C, Mass Spec Grade
CompanyPromega
Price Supplier Page View Company Product Page
Catalog NumberVA1170
Size20microg
TypeEnzyme

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Promega

2800 Woods Hollow Rd

Madison, WI 53711

United States

Phone: Tel: +1 (608) 274-4330
Fax: +1 (608) 277-2516

Email: custserv@promega.com
techserv@promega.com

Company Profile

Website: http://www.promega.com

(1) Plasma extracellular vesicle proteomics nominates candidate biomarkers of 177Lu-PSMA-617 outcomes in metastatic prostate cancer patientsCell Reports MedicineMay 19, 2026Ali T. Arafa, Ella Boytim, Megan L. Ludwig, Lily Kollitz, Tianzhong Yang, Kathleen M. Storey, Stuart Bloom, Gautam Jha, Ian Okazaki, Charles J. Ryan, et al.inhibitor,Millipore Sigma,Cat# 11836153001 Phosphatase inhibitor,ThermoFisher,Cat# 78428 LysC,Promega,Cat# VA1170 Trypsin,Worthington,Cat# LS003740 ,, Critical commercial assays,Critical commercial assays,Critical

(2) Cepharanthine inhibits lysosomes and induces apoptosis in triple‑negative breast cancer cellsMol Med RepMay 4, 2026Juan Li, Siqin Zhou, Yu Wang, Guanghan Ou, Danli Yang, Liwen Shenfollowing pH adjustment to a pH of 9, digested sequentially with C-terminal lysin (1:100; cat. no. VA1170; Promega Corporation) at 37°C for 4 h and trypsin (1:100) at 37°C overnight. The isolated peptides were

(3) TIE-UP-SIN: A Method for Enhanced Identification of Protein–Protein InteractionsBIO-PROTOCOLApril 20, 2026Maximilian Schedlowski, Stephan Michalik, Tilly Hoffmüller, Marco Harms, Leif Steil, Kristin Surmann, Christian Hentschker, Manuela Gesell Salazar, Uwe Völker, Alexander Rederfor LC-MS (Merck, catalog number: 1000291000) 18. Lys-C, mass spec grade (Promega, catalog number: VA1170) 19. Sequencing-grade modified trypsin (Promega, catalog number: V5111) 20. 2xiRT kit (Biognosys, catalog

(4) TIE-UP-SIN: A Method for Enhanced Identification of Protein–Protein InteractionsBio ProtocApril 20, 2026Maximilian Schedlowski, Stephan Michalik, Tilly Hoffmüller, Marco Harms, Leif Steil, Kristin Surmann, Christian Hentschker, Manuela Gesell Salazar, Uwe Völker, Alexander Rederfor LC-MS (Merck, catalog number: 1000291000) 18. Lys-C, mass spec grade (Promega, catalog number: VA1170) 19. Sequencing-grade modified trypsin (Promega, catalog number: V5111) 20. 2xiRT kit (Biognosys, catalog

(5) Deep visual proteomics uncovers nociceptor diversity and pain targetsNat CommunApril 11, 2026Sampurna Chakrabarti, Anuar Makhmut, Atena Mohammadi, Wenhan Luo, Lin Wang, Gary R. Lewin, Fabian CosciaVWR, cat. 83640.290) was added and incubated for 60 min at 75 °C. Next, 1 μl of LysC (Promega, cat. no VA1170) (2 ng/μL) was added, followed by a 2 h digestion at 37 °C. Then, 1 μl of trypsin (Promega, cat.no.

(6) Polystyrene microplastic-induced pathophysiology is driven by disruption of efferocytosisImmunityFebruary 24, 2026Ana C. Codo, Jesus E. Romero-Pichardo, Zhaoquan Wang, Mariano A. Aufiero, Tomi Lazarov, Waleska Saitz Rojas, Nicole S. Walker, Achuth Nair, Roger F. Cole, Savannah Adkins, et al.acid,Sigma-Aldrich,Cat#5.33002 "EPPS, 0.2M buffer soln., pH 8.5",Thermo Scientific,Cat#J61476.AK "Lys-C, Mass Spec Grade",Promega,Cat#VA1170 Sequencing Grade Trypsin,Promega,Cat#V5111 CDS Analytical™ Empore™ C18 StageTips,CDS Analytical,Cat#13-110-055 Aurora

(7) Structural and mechanistic analysis of covalent ligands targeting the RNA-binding protein NONOCell Chemical BiologyFebruary 19, 2026Garrett L. Lindsey, Thomas K. Hockley, Alejandro Villa Gomez, Andrew C. Marshall, William R. Brothers, Colin T. Finney, Jacob Gross, Archa H. Fox, Gene W. Yeo, Bruno Melillo, et al.271606 Sequencing grade modified trypsin,Promega,Cat#: V5111 "Lys-C, Mass Spec Grade",Promega,Cat#: VA1170 Streptavidin agarose resin,Fisher Scientific,Cat#: 20353 Tween 20,Fisher Bioreagents,Cat#: BP337-500 Triton X-100

(8) Faecal metaproteomics analysis reveals a high cardiovascular risk profile across healthy individuals and heart failure patientsGut MicrobesDecember 31, 2025Chaoran Yang, Leticia Camargo Tavares, Han-Chung Lee, Joel R. Steele, Rosilene V. Ribeiro, Anna L. Beale, Stephanie Yiallourou, Melinda J. Carrington, David M. Kaye, Geoffrey A. Head, et al.using Trypsin (Promega, V528X) at a 1:50 wt:wt ratio alongside Lys-C at a 1:25 wt:wt ratio (Promega, VA1170) at 37°C for 16 hours. Digestion efficiency was greater than 89% for this analysis. TMT labeling and

(9) Simulated Microgravity-Induced Changes in SUMOylation and Protein Expression in Saccharomyces cerevisiaeInternational Journal of Molecular SciencesDecember 19, 2025Jeremy A. Sabo, Steven D. HartsonLys-C using the Promega protocol for Lys-C digestion of mixed protein samples (Madison, WI, USA, cat.# VA1170). Protein pellets were resuspended in 8 M urea, 50 mM Tris, pH 8 and quantified using a nanodrop. Methyl

(10) Spatial proteomics of ovarian cancer precursors delineates early disease changes and drug targetsMolecular Systems BiologyNovember 13, 2025Anuar Makhmut, Mihnea P Dragomir, Sonja Fritzsche, Markus Moebs, Wolfgang D Schmitt, Eliane T Taube, Fabian Cosciaother reagents" n-Dodecyl-beta-Maltoside (DDM),Sigma-Aldrich,D4641-500MG Endoproteinase LysC,Promega,VA1170 Proteomics grade modified trypsin,Promega,V5117 Tris(2-carboxyethyl)phosphine hydrochloride,Sigma-Aldrich,C4706-2G Acetonitrile

(11) Mapping Dysfunctional Protein-Protein Interactions in DiseaseJournal of visualized experiments : JoVEOctober 24, 2025Rodina A, Erdjument-Bromage H, Monetti M, Li Z, Chakrabarty S, Wang S, Digwal CS, Tuffery L, Panchal P, Sharma S, et al.(IAA),Sigma-Aldrich,I1149-5G,Toxic Low-protein binding microcentrifuge tubes,Sarstedt,72.706.600, Lys-C protease,Promega,VA1170,LC/MS-grade Magnesium Chloride,Sigma-Aldrich,63068,Molecular biology grade MaxQuant (v2.1.1.0),Max-Planck-Institute

(12) Protocol for spatial proteomic profiling of tonsil cancer microenvironments using multiplexed imaging-powered deep visual proteomicsSTAR ProtocolsSeptember 1, 2025Xiang Zheng, Andreas Mund, Matthias Mannbicarbonate,Merck,T7408 n-dodecyl-β-D-maltoside,Merck,850520P Acetonitrile,Thermo Fisher Scientific,A955-1 LysC,Promega,VA1170 Trypsin,Promega,V5280 Trifluoroacetic acid,VWR,153112E Formic acid,Merck,5.33002 "Formaldehyde,

(13) Global analysis of protein and small-molecule substrates of ubiquitin-like proteins (UBLs)Molecular & Cellular ProteomicsApril 18, 2025Guang-Can Shao, Zhen-Lin Chen, Shan Lu, Qing-Cui Wu, Yao Sheng, Jing Wang, Yan Ma, Jian-Hua Sui, Hao Chi, Xiang-Bing Qi, et al.SUMO-spermidine conjugation also occurs in mice and humans. Experimental Procedures Reagents Lys-C (cat. #VA1170), trypsin (cat. #V5280), and Asp-N (cat. #V1621) were purchased from Promega. The anti-HA antibody was

(14) Novel insights into the molecular nature of neurofilament light polypeptide species in cerebrospinal fluidBrain CommunApril 2, 2025Bruno Becker, Johan Gobom, Gunnar Brinkmalm, Ulf Andreasson, Francisco J Meda, Henrik Zetterberg, Kaj Blennowpreparation. Digestion was performed in a volume of 50 µL using 1.5 µL of Lys-C (200 ng/µL; Promega, # VA1170) over night at 37°C. Finally, the digestion was stopped by addition of formic acid to 1%. Lys-C digestion

(15) Potential Role of Menstrual Fluid-Derived Small Extracellular Vesicle Proteins in Endometriosis PathogenesissJ Extracell VesiclesMarch 17, 2025Shanti Gurung, Jacqueline Piskopos, Joel Steele, Ralf Schittenhelm, Anup Shah, Fiona L. Cousins, Thomas T. Tapmeier, Caroline E. Gargettmin. Trypsin (Promega, #V528X) was added at a 1:50 wt:wt ratio alongside Lys‐C at a 1:25 wt:wt ratio (#VA1170, Promega), and samples were incubated at 37°C overnight. Digestion was halted, and SDC was precipitated

(16) Revisiting phosphoregulation of Cdc25C during M-phase inductioniScienceJanuary 17, 2025Tan Tan, Chuanfen Wu, Ruoning Wang, Bih-Fang Pan, David Hawke, Fumin Yin, Zehao Su, Boye Liu, Sue-Hwa Lin, Wei Zhang, et al.modified trypsin,Promega Corporation,Cat# V5111 "Lys-C, Mass Spec Grade",Promega Corporation,Cat# VA1170 ,, "Chemicals, peptides, and recombinant proteins","Chemicals, peptides, and recombinant proteins","Chemicals,

(17) An LC–MS-based designated comparison method with similar performance to the Lp(a) reference measurement procedure to guide molar Lp(a) standardizationClinical ProteomicsDecember 1, 2024Nina M. Diederiks, L. Renee Ruhaak, Fred P. H. T. M. Romijn, Mervin M. Pieterse, Nico P. M. Smit, Christa M. Cobbaertthis section. Materials Sequencing grade modified trypsin (V5111) and mass spectrometry grade Lys-C (VA1170) were acquired from Promega (Leiden, The Netherlands). LC–MS grade methanol (MeOH) was purchased from

(18) The cyclimids: Degron-inspired cereblon binders for targeted protein degradationCell Chemical BiologyJune 20, 2024Saki Ichikawa, N. Connor Connor, Wenqing Payne, Chia-Fu Chang, Nandini Vallavoju, Spencer Frome, Hope A. Flaxman, Ralph Mazitschek, Christina M. WooTR-FRET,Greiner,Cat# 781207 BsmBI,New England BioLabs,Cat# R0739S "Lys-C, Mass Spec Grade",Promega,Cat# VA1170 "Trypsin, sequencing grade modified",Promega,Cat# V5111 Pierce Peptide Desalting Spin Column,Thermo,Cat#

(19) TDP-43-stratified single-cell proteomics of postmortem human spinal motor neurons reveals protein dynamics in amyotrophic lateral sclerosisCell ReportsJanuary 23, 2024Amanda J. Guise, Santosh A. Misal, Richard Carson, Jen-Hwa Chu, Hannah Boekweg, Daisha Van Der Watt, Nora C. Welsh, Thy Truong, Yiran Liang, Shanqin Xu, et al.LC-MS Acetonitrile,Honeywell,349674X4L "Trypsin, MS-grade",Promega,V5280 "Lys-C, MS-grade",Promega,VA1170 Bond Primary Antibody Diluent,Advanced Cellular Diagnostics,AR9352 Bond Polymer Refine Detection Kit,Advanced

(20) Metabolic remodeling in cardiac hypertrophy and heart failure with reduced ejection fraction occurs independent of transcription factor EB in miceFrontiers in Cardiovascular MedicineJanuary 8, 2024Niklas Dörmann, Elke Hammer, Karlotta Struckmann, Julia Rüdebusch, Kirsten Bartels, Kristin Wenzel, Julia Schulz, Stefan Gross, Stefan Schwanz, Elisa Martin, et al.mM iodoacetamide, Cat. no.: I1149, Merck KGaA, for 30 min at 37°C). Proteolysis with LysC (Cat. no.: VA1170, 1:100 for 3 h at 37°C) followed by tryptic digestion over night at 37°C (Cat. no.: VA9000, both from

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Lys-C, Mass Spec Grade

Lys-C, Mass Spec Grade from Promega

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Promega

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Lys-C, Mass Spec Grade from Promega

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