Description
Description: Recombinant human RET/PTC3, the fusion protein [NCOA4 (1-238)-RET (713-end)], was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag.
Scientific Background: RET/PTC3 is fused of RET and the activating NCOA4 gene by intrachromosomal paracentric inversions in chromo-some 10 (1). Likes RET/PTC1, it is the most frequent RET rearrangements in papillary thyroid carcinoma (PTC) (2), especially in radiation-induced tumours. The RET/PTC3 rearrangements may be typical for radiation-induced childhood PTC with a short latency period (3). The RET/PTC rearrangements also have been shown in benign thyroid lesions, including Hashimoto's thyroiditis (HT)