Description
Description: Full-length recombinant human MEP50 (WDR77) protein was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag.
Scientific Background: Methylosome protein 50 (MEP50) is a component of methylosome that contains the methyltransferase JBP1 (PRMT5) and the pICln protein. The methylosome functions to modify specific arginines to dimethylarginines in the arginine- and glycine-rich domains of several spliceosomal Sm proteins. Within the methylosome, MEP50 binds protein substrates and activates the oncogenic protein arginine methyl transferase 5 (PRMT5)