Description
Description: Recombinant human EHMT1 (895-end) was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag.
Scientific Background: Euchromatic histone-lysine N-methyltransferase 1 (EHMT1) is in charge of the mono- and demethylation of lysine-9 of histone H3. EHMT1 is found in a multimeric protein complex containing chromatin modifiers, which contributes to silencing of MYC- and E2F-responsive genes during G0 phase of cell cycle (1). EHMT1 plays a role in epithelial-to-mesenchymal transition and tumor invasion by repressing expression of tumour suppressor genes (2). Additionally, EHMT1 also methylates non-histone proteins such as lysine-373 of p53 (3)