Description
Description: Recombinant human fusion TFG (1-193)-TRKA (399-end) protein was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag.
Scientific Background: TFG-TRKA (TRK-T3) is a chimeric cytoplasmic oncoprotein isolated from a papillary thyroid tumor. This oncoprotein is the consequence of a t (1, 3) translocation juxtaposing the tyrosine kinase domain of the NTRK1 receptor to the N-terminal portion of a protein encoded by TFG (TRK-fused gene), a novel gene first discovered in the rearranged form. TRK-T3 fusion oncoprotein displays a constitutive tyrosine kinase activity resulting in its capability to transform mouse NIH3T3 cells. The TFG portion of TRK-T3 contains a coiled-coil domain most likely responsible for the constitutive, ligand-independent activation of the receptor tyrosine kinase activity through TRK-T3 oligomerization