Description
Description: Recombinant human PDE4C (287-end) was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag.
Scientific Background: PDE4C is a member of the phosphodiesterase (PDE) family that is cAMP-specific and has homology to the 'dunce' gene of Drosophila. The 'dunce' gene is one of several genes critical for normal learning and memory in the fly. Homozygous mutations in 'dunce' cause defective short-term memory and behavioral defects (1). PDE4C is assigned to human chromosome 19 by Southern analyses while in situ hybridization localized the PDE4C gene to 19p13.1. PDE4C has been shown to be a substrate for phosphorylation by ERK2 within the C-terminal portion of the PDE4C catalytic unit and this phosphorylation can regulate the activity of PDE4C (2)