rAsp-N, Mass Spec Grade VA1160 from Promega

Product Specs
ItemrAsp-N, Mass Spec Grade
CompanyPromega
Price Supplier Page View Company Product Page
Catalog NumberVA1160
Size10microg
TypeEnzyme

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Promega

2800 Woods Hollow Rd

Madison, WI 53711

United States

Phone: Tel: +1 (608) 274-4330
Fax: +1 (608) 277-2516

Email: custserv@promega.com
techserv@promega.com

Company Profile

Website: http://www.promega.com

(1) Cas13d-mediated isoform-specific RNA knockdown with a unified computational and experimental toolboxNat CommunJuly 29, 2025Megan D. Schertzer, Andrew Stirn, Keren Isaev, Laura Pereira, Stella H. Park, Anjali Das, Aline Réal, Erin D. Jeffery, Claire Harbison, Hans-Hermann Wessels, et al.(FASP) adapted from ref. 80: one using Trypsin (Promega, V5280) and the other using Asp-N (Promega, VA1160), 1 µg. Each digestion yielded ~20 µg of peptide as assayed by NanoDrop A280 (ThermoFisher Scientific).

(2) Virus-Receptor Interactions of Glycosylated SARS-CoV-2 Spike and Human ACE2 ReceptorbioRxivApril 26, 2023Peng Zhao, Jeremy L. Praissman, Oliver C. Grant, Yongfei Cai, Tianshu Xiao, Katelyn E. Rosenbalm, Kazuhiro Aoki, Benjamin P. Kellman, Robert Bridger, Dan H. Barouch, et al.,Sigma Aldrich,Cat#I1149 Trypsin,Promega,Cat#V5111 Lys-C,Promega,Cat#V1671 Arg-C,Promega,Cat#V1881 Glu-C,Promega,Cat#V1651 Asp-N,Promega,Cat#VA1160 Endoglycosidase H,Promega,Cat#V4871 PNGaseF,Promega,Cat#V4831 Chymotrypsin,Athens Research and Technology,Cat#16-19-030820 Alpha

(3) Oxygen level regulates N-terminal translation elongation of selected proteins through deoxyhypusine hydroxylationCell reportsMay 24, 2022Zhang Y, Su D, Zhu J, Wang M, Zhang Y, Fu Q, Zhang S, Lin H.BP172-5 Iodoacetamide,Fisher Scientific,Cat# 122271000 Phosphoric acid,Fisher Scientific,Cat# A260-500 AspN,Promega,Cat# VA1160 Formic acid,Fisher Scientific,Cat# A117-50 Acetonitrile,Fisher Scientific,Cat# A955-4 "Lysine-13C6,15N2

(4) Comparison of N-linked glycosylation on hemagglutinins derived from chicken embryos and MDCK cells: a case of the production of a trivalent seasonal influenza vaccineApplied Microbiology and BiotechnologyMay 1, 2021Jingqi Li, Sixu Liu, Yanlin Gao, Shuaishuai Tian, Yu Yang, Ningning Madetermined by peptide mapping of deglycosylated HA. Multiple enzymes were used, including trypsin (Promega, VA1160, US), chymotrypsin (Sigma, US), or a combination of trypsin (Sigma, US) and Glu-C (Sigma, US). The digested

(5) Glycomics-informed glycoproteomic analysis of site-specific glycosylation for SARS-CoV-2 spike proteinSTAR ProtocolsDecember 30, 2020Katelyn E. Rosenbalm, Michael Tiemeyer, Lance Wells, Kazuhiro Aoki, Peng Zhaoreagent",Sigma-Aldrich,320331 Dithiothreitol,Sigma-Aldrich,43815 Iodoacetamide,Sigma-Aldrich,I1149 Trypsin,Promega,V5111 Lys-C,Promega,V1671 Arg-C,Promega,V1881 Glu-C,Promega,V1651 Asp-N,Promega,VA1160 Endoglycosidase H,Promega,V4871 PNGaseF,Promega,V4831 Chymotrypsin,Athens Research and Technology,16-19-030820 Alpha

(6) ProAlanase is an Effective Alternative to Trypsin for Proteomics Applications and Disulfide Bond MappingMolecular & Cellular ProteomicsDecember 1, 2020Diana Samodova, Christopher M. Hosfield, Christian N. Cramer, Maria V. Giuli, Enrico Cappellini, Giulia Franciosa, Michael M. Rosenblatt, Christian D. Kelstrup, Jesper V. OlsenProAlanase prototype (Promega) for 2 h at pH 1.5, 37 °C or with Trypsin (Sigma-Aldrich), rAsp-N (Promega, VA1160) and Glu-C (Sigma-Aldrich) for 18 h at pH 8.5, 37 °C. After spinning down for 5 min at 17000 × g, peptides

(7) ProAlanase is an Effective Alternative to Trypsin for Proteomics Applications and Disulfide Bond MappingMol Cell ProteomicsOctober 5, 2020Diana Samodova, Christopher M. Hosfield, Christian N. Cramer, Maria V. Giuli, Enrico Cappellini, Giulia Franciosa, Michael M. Rosenblatt, Christian D. Kelstrup, Jesper V. OlsenProAlanase prototype (Promega) for 2 h at pH 1.5, 37 °C or with Trypsin (Sigma-Aldrich), rAsp-N (Promega, VA1160) and Glu-C (Sigma-Aldrich) for 18 h at pH 8.5, 37 °C. After spinning down for 5 min at 17000 × g, peptides

(8) Virus-Receptor Interactions of Glycosylated SARS-CoV-2 Spike and Human ACE2 ReceptorCell Host & MicrobeOctober 1, 2020Peng Zhao, Jeremy L. Praissman, Oliver C. Grant, Yongfei Cai, Tianshu Xiao, Katelyn E. Rosenbalm, Kazuhiro Aoki, Benjamin P. Kellman, Robert Bridger, Dan H. Barouch, et al.,Sigma Aldrich,Cat#I1149 Trypsin,Promega,Cat#V5111 Lys-C,Promega,Cat#V1671 Arg-C,Promega,Cat#V1881 Glu-C,Promega,Cat#V1651 Asp-N,Promega,Cat#VA1160 Endoglycosidase H,Promega,Cat#V4871 PNGaseF,Promega,Cat#V4831 Chymotrypsin,Athens Research and Technology,Cat#16-19-030820 Alpha

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rAsp-N, Mass Spec Grade

rAsp-N, Mass Spec Grade from Promega

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Promega

Citations (8)

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rAsp-N, Mass Spec Grade from Promega

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