HiBiT Control Protein N3010 from Promega

Product Specs
ItemHiBiT Control Protein
CompanyPromega
Price Supplier Page View Company Product Page
Catalog NumberN3010
Size100microl

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Promega

2800 Woods Hollow Rd

Madison, WI 53711

United States

Phone: Tel: +1 (608) 274-4330
Fax: +1 (608) 277-2516

Email: custserv@promega.com
techserv@promega.com

Company Profile

Website: http://www.promega.com

(1) Optimization and Characterization of SHIP1 Ligands for Cellular Target Engagement and Activity in Alzheimer’s Disease ModelsbioRxivJanuary 5, 2026Cynthia D. Jesudason, Claudia Rangel-Barajas, Colin J. Beach, Daniel E. Beck, Isaac H. Caballero-Floran, W. Brent Clayton, Lais Da Silva, Juliane C. David, Suzanne Doolen, Alexandra N. Faulkner, et al.or it’s luminescence activity, an assay was performed in parallel using the HiBiT control protein (N3010, Promega). Given the role of SHIP1 regulating phosphatidylinositol signaling, ligands that engage this

(2) Regulation of the orphan G-protein–coupled receptor GPRC5B by MLC1 and the cell adhesion molecule GlialCAM in megalencephalic leukoencephalopathyJournal of Biological ChemistryJanuary 1, 2026Guillem Pont-Espinós, Adrià Pla-Casillanis, Laura Ferigle, Marta Alonso-Gardón, Marc González-Subías, Xabier Elorza-Vidal, Héctor Gaitán-Peñas, Ekaitz Errasti-Murugarren, Andy Chevigne, Tania López-Hernández, et al.Nano-Glo HiBiT Lytic detection system following the supplier’s instructions. The HiBiT control protein (N3010; Promega) was used in place of the LgBiT protein provided in the kit. Split-TEV experiments Protein–protein

(3) The Endo-GeneScreen platform identifies drug-like probes that regulate endogenous protein levels within physiological contextsNat CommunDecember 9, 2025Preston Samowitz, Laszlo Radnai, Thomas Vaissiere, Sheldon D. Michaelson, Camilo Rojas, Ryan Mitchell, Murat Kilinc, Austin Edwards, Justin Shumate, Richard Hawkins, et al.Nano-Glo® HiBIT Extracellular Detection System (Promega: N2420), a HiBIT control protein (Promega: N3010) was diluted to 100 pM in buffer containing 0.1% BSA-PBS and 10 µL was dispensed into black opaque 384-well

(4) Conservation and divergence of regulatory architecture in nitrate-responsive plant gene circuitsThe Plant CellJune 4, 2025Chao Bian, Gozde S Demirer, M Tufan Oz, Yao-Min Cai, Sam Witham, G Alex Mason, Zhengao Di, Florian Deligne, Ping Zhang, Rachel Shen, et al.Nano-Glo HiBiT Extracellular Detection System (N2420, Promega, Madison, WI) and HiBiT Control Protein (N3010, Promega). We were unable to obtain the soluble protein of AtARF9. Details of all plasmids are available

(5) Probing RAS Function Using Monobody and NanoBiT TechnologiesMethods in Molecular BiologyNovember 30, 2024Michael Whaby, Rakesh Sathish Nair, John P. O’BryanControl VectorNanoGlo Live Cell Substrate (LCS)NanoGlo LCS Dilution Buffer HiBiT Control Protein (Cat # N3010, Promega) 10% Triton-X 100 HA-tagged RAS expression vectors (pCGN-based)—available by request from

(6) mRNAid, an open-source platform for therapeutic mRNA design and optimization strategiesNAR Genom BioinformMarch 12, 2024Nikita Vostrosablin, Shuhui Lim, Pooja Gopal, Kveta Brazdilova, Sushmita Parajuli, Xiaona Wei, Anna Gromek, David Prihoda, Martin Spale, Anja Muzdalo, et al.determined using the Envision plate reader, and values were normalized to a HiBiT-control protein (Promega, N3010) Western blot analysis A total of 0.08 million MIA PaCa-2 cells were seeded per well in a 24-well

(7) Tuning Plant Promoters Using a Simple Split Luciferase Method to Assess Transcription Factor-DNA InteractionsACS Synthetic BiologyNovember 17, 2023Y.-M. Cai, S. Witham, N. J. PatronNano-Glo HiBiT Extracellular Detection System (N2420, Promega, Madison, WI) using the HiBiT Control Protein (20 μM, N3010, Promega) as a standard. EMSA EMSA was performed as previously described,6 except that probes were

(8) The HLA class I immunopeptidomes of AAV capsid proteinsFrontiers in ImmunologyAugust 16, 2023Carlos A. Brito-Sierra, Megan B. Lannan, Laurent P. Malherbe, Robert W. Siegelquantification was determined by a standard curve generated using the HiBiT control protein (Promega # N3010). Protein expression was measured at 0, 2, 4, 6 8, 24, 48, 72 and 96 hours post transfection. For the

(9) Probing expression of E-selectin using CRISPR-Cas9-mediated tagging with HiBiT in human endothelial cellsiScienceJuly 21, 2023Lydia Ogrodzinski, Simon Platt, Joelle Goulding, Cameron Alexander, Tracy D. Farr, Jeanette Woolard, Stephen J. Hill, Laura E. Kilpatrick1:100 Endurazine and 50nM LgBiT, (incubated for 15 h 37°C)) by addition of 1 nM purified HiBiT protein (N3010, Promega USA) (5min, 37°C). Bioluminescence microscopy Genome edited HiBiT E-selectin HUVEC/TERT2

(10) A cell-free platform to measure coronavirus membrane fusionSTAR ProtocolsJune 1, 2023Thomas Kicmal, Enya Qing, Grant M. Hawkins, Alexandria Wilcox, Tom Gallagher≥90% purity (VSGWALFKKIS),Genscript (Fast Peptide Synthesis),N/A HiBiT control protein,Promega,Cat#N3010 LgBiT protein (included in Nano-Glo HiBiT Extracellular Detection System kit),Promega,Cat#N401A Passive

(11) Programmable protein delivery with a bacterial contractile injection systemNatureApril 1, 2023Kreitz J, Friedrich MJ, Guru A, Lash B, Saito M, Macrae RK, Zhang F.payload proteins were run alongside a titration of a control protein (HiBiT control protein; Promega N3010); band intensities (quantified with ImageJ) were then compared to determine the approximate quantity

(12) Biofoundry-assisted expression and characterization of plant proteinsSynthetic biology (Oxford, England)January 1, 2021Dudley QM, Cai YM, Kallam K, Debreyne H, Carrasco Lopez JA, Patron NJ.11836170001 (Roche, Basel, Switzerland) protease inhibitor tablet). A standard curve of HiBiT Control Protein N3010 (Promega) was diluted in PLB + PI at concentrations ranging from 0.01 to 2 nM. Luminescence reactions

(13) Monitoring Allosteric Interactions with CXCR4 Using NanoBiT Conjugated NanobodiesCell chemical biologyOctober 15, 2020Soave M, Heukers R, Kellam B, Woolard J, Smit MJ, Briddon SJ, Hill SJ.E2311 Geneticin™ (G418),ThermoFisher,Cat# 10131035 HiBiT-HaloTag control peptide,"Promega, Wisconsin, USA",Cat# N3010 "N,N’-Dicyclohexylcarbamimidothioic acid (5,6- dihydro-6,6-dimethylimidazo[2,1-b]thiazol-3-yl)methyl

(14) CRISPR-Mediated Protein Tagging with Nanoluciferase to Investigate Native Chemokine Receptor Function and Conformational ChangesCell chemical biologyMay 21, 2020White CW, Caspar B, Vanyai HK, Pfleger KDG, Hill SJ.(G418),ThermoFisher,Cat# 10131035 "HiBiT-Halotag, control peptide","Promega (Wisconsin, USA)",Cat# N3010 Isoprenaline hydrochloride,Sigma Aldrich,Cat# I6504 Phosphate Buffered Saline (PBS),Sigma Aldrich,Cat#

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HiBiT Control Protein

HiBiT Control Protein from Promega

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HiBiT Control Protein from Promega

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