Recombinant Human HE4 Protein, HEK293 Lysate 230-10002-100 from RayBiotech

Product Specs
ItemRecombinant Human HE4 Protein, HEK293 Lysate
CompanyRayBiotech
Price $150.00Supplier Page View Company Product Page
Catalog Number230-10002-100
Size100 µg
ApplicationsWB, ELISA
NCBI Full Gene NameWAP four-disulfide core domain 2
NCBI Gene AliasesHE4, dJ461P17.6, WAP5, EDDM4
Conjugate/TagC-terminal 6x histidine tag
Source/Expression SystemHEK293 cells
ConcentrationLot specific (see the label on the vial)
FormatLyophilized powder
Molecular WeightThe predicted size of HE4 protein is 12 kDa. The 20-kDa band may represent N-glycosylated form (Ronny Drapkin, et al. Cancer Res 2005, 65(6): 2162-9)
SpeciesHuman
Molecule NameHE4, WAP5, WAP four-disulfide core domain protein 2
PurityUnpurified cell lysate. HEK293 cells transfected with expression vectors harboring target gene were harvested and washed with PBS twice. The cell pastes were re-suspended with ice-cold PBS containing mammalian cell protease inhibitor cocktail and further lysed with freeze-thaw cycles. After clarifying with 20,000 g centrifugation at 4°C for 30 min, the lysate was aliquoted, lyophilized, and stored at -80°C immediately. Protein concentration was determined by BCA kit (Thermo Scientific, Inc.) using BSA as protein standard. The gene overexpression in lysate was confirmed by Western blotting using anti-His tag antibody and/or target-specific antibodies and the lysate derived from HEK293 cells transfected with the empty expression vector was used as a negative control