Recombinant Human Versican Protein, HEK293 Lysate 230-10237-100 from RayBiotech

Product Specs
ItemRecombinant Human Versican Protein, HEK293 Lysate
CompanyRayBiotech
Price $150.00Supplier Page View Company Product Page
Catalog Number230-10237-100
Size100 µg
Conjugate/TagN-terminal 6x histidine tag
FormatLyophilized powder
Molecular WeightRecombinant Human Versican has a calculated molecular mass of 39 kDa. The actual molecular weight may increase slightly due to the potential post-modifications (PTMs)
SpeciesHuman
Molecule NameVersican, Chondroitin Sulfate Proteoglycan 2, Chondroitin Sulfate Proteoglycan Core Protein 2, Glial Hyaluronate-Binding Protein, Large Fibroblast Proteoglycan, Versican Proteoglycan, CSPG2, GHAP, PG-M, ERVR, WGN1, WGN
Source/Expression SystemHEK293 cells
ConcentrationDetermined by Pierce BCA protein assay kit
NCBI Full Gene Nameversican
NCBI Gene AliasesCSPG2, ERVR, GHAP, PG-M, WGN, WGN1
PurityUnpurified cell lysate. HEK293 cells transfected with expression vectors harboring target gene were harvested and washed with PBS twice. The cell pastes were re-suspended with ice-cold PBS containing mammalian cell protease inhibitor cocktail and further lysed with freeze-thaw cycles. After clarifying with 20,000 g centrifugation at 4°C for 30 min, the lysate was aliquoted, lyophilized, and stored at -80°C immediately. Protein concentration was determined by BCA kit (Thermo Scientific, Inc.) using BSA as protein standard. The gene overexpression in lysate was confirmed by Western blotting using anti-His tag antibody and/or target-specific antibodies and the lysate derived from HEK293 cells transfected with the empty expression vector was used as a negative control