Rapid Digestion Kit---Trypsin VA1060 from Promega

Product Specs
ItemRapid Digestion Kit---Trypsin
CompanyPromega
Price Supplier Page View Company Product Page
Catalog NumberVA1060
Size100microg

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Promega

2800 Woods Hollow Rd

Madison, WI 53711

United States

Phone: Tel: +1 (608) 274-4330
Fax: +1 (608) 277-2516

Email: custserv@promega.com
techserv@promega.com

Company Profile

Website: http://www.promega.com

(1) Opposing regulation of the STING pathway in hepatic stellate cells by NBR1 and p62 determines the progression of hepatocellular carcinomaMolecular CellDecember 5, 2024Sadaaki Nishimura, Juan F. Linares, Antoine L’Hermitte, Angeles Duran, Tania Cid-Diaz, Anxo Martinez-Ordoñez, Marc Ruiz-Martinez, Yotaro Kudo, Antonio Marzio, Mathias Heikenwalder, et al.Kit,Agilent,Cat# 200523 Quick-RNA Miniprep Kit,Zymo Research,Cat# R1054 Rapid Digestion,Promega,Cat# VA1060 "RPMI 1640 Medium, GlutaMAX supplement",Gibco,Cat# 61870036 RNAlater Stabilization Solution,Invitrogen,Cat#

(2) The Addition of Transcriptomics to the Bead-Enabled Accelerated Monophasic Multi-Omics Method: A Step toward Universal Sample PreparationAnalytical ChemistryNovember 19, 2024Joshua D. Breidenbach, Emilio S. Rivera, Tara Harvey, Abigale S. Mikolitis, Chi Yen Tseng, Claire K. Sanders, Emilia Solomon, Cheryl D. Gleasner, M. Grace Thornhill, Kes A. Luchini, et al.digestion buffer containing a 1:3 ratio of 20 mM IAA, 5 mM TCEP, and Rapid Digest Buffer (Promega, Cat. VA1060; Madison, WI), and vortexed for 10 s. To facilitate rapid protein digestion, rapid Trypsin Gold (Promega,

(3) Optimal conditions for carrying out trypsin digestions on complex proteomes: From bulk samples to single cellsJournal of ProteomicsApril 1, 2024M. Shahid Mansuri, Shveta Bathla, TuKiet T. Lam, Angus C. Nairn, Kenneth R. Williamsenabled them to carry out a one hour digest at 70°C [53]. Promega’s “Rapid Digestion Trypsin” (product VA1060) is used with a proprietary “Resuspension Buffer” that enables trypsin digestions to be carried out

(4) A fully automated FAIMS-DIA mass spectrometry-based proteomic pipelineCell Reports MethodsOctober 23, 2023Luke Reilly, Erika Lara, Daniel Ramos, Ziyi Li, Caroline B. Pantazis, Julia Stadler, Marianita Santiana, Jessica Roberts, Faraz Faghri, Ying Hao, et al.Triton X-100,MilliporeSigma,Cat# X100 "Trypsin/Lys-C Mix, Mass Spec Grade",Promega,Cat# V5073 Rapid trypsin,Promega,Cat# VA1060 SMART digest trypsin,Thermo Scientific,Cat# 60113-101 TWEEN 20,MilliporeSigma,Cat# P9416 "0.5M EDTA, pH 8.0

(5) Quality Control—A Stepchild in Quantitative Proteomics: A Case Study for the Human CSF ProteomeBiomoleculesMarch 7, 2023Svitlana Rozanova, Julian Uszkoreit, Karin Schork, Bettina Serschnitzki, Martin Eisenacher, Lars Tönges, Katalin Barkovits-Boeddinghaus, Katrin Marcusin-solution digestion of CSF samples, a Rapid Digestion Trypsin kit (Promega, Madison, WI, USA, Cat#VA1060) was used according to the manufacturer’s protocol. Briefly, 25 μg of CSF protein was mixed with 3 sample

(6) Preclinical evaluation of proteolytic targeting of LCK as a therapeutic approach in T cell acute lymphoblastic leukemiaScience Translational MedicineAugust 24, 2022Jianzhong Hu, Jamie Jarusiewicz, Guoqing Du, Gisele Nishiguchi, Satoshi Yoshimura, John C. Panetta, Zhenhua Li, Jaeki Min, Lei Yang, Divyabharathi Chepyala, et al.1:100 w/w) at 21°C for 3 h, then diluted 4-fold to reduce urea to 2 M for trypsin digestion (Promega, #VA1060, 1:50 w/w) at 21°C overnight. Reduction and alkylation were carried out by adding DTT and IAA. Trifluoroacetic

(7) Comparison of anti-peptide and anti-protein antibody-based purification techniques for detection of SARS-CoV-2 by targeted LC-MS/MSAdvances in Sample PreparationMay 16, 2022Anthony Maus et al.

(8) Targeted Detection of SARS-CoV-2 Nucleocapsid Sequence Variants by Mass Spectrometric Analysis of Tryptic PeptidesJournal of proteome researchJanuary 7, 2022Maus A, Renuse S, Kemp J, Madugundu AK, Vanderboom PM, Blommel J, Jerde C, Dasari S, Kipp BR, Singh RJ, et al.0.002% Z3–16 in water. The purified sample was immediately trypsin digested (rapid digest kit, Catalog#VA1060, Promega, Madison, WI). The sample eluent was mixed with 300 μL of digest buffer followed by the addition

(9) Modifications of IL-6 by Hypochlorous Acids: Effects on Receptor BindingACS OmegaDecember 28, 2021Lori I. Robins, Erika K. Keim, Deborah B. Robins, John S. Edgar, John S. Meschke, Philip R. Gafken, Jeffrey F. WilliamsMass Spectrometry IL-6 and treated IL-6 samples were prepared following the rapid digestion-trypsin protocol (Promega, VA1060). Three HOCl-treated samples were analyzed, single analyses were completed on HOBr- and NCT-treated

(10) A mass spectrometry-based targeted assay for detection of SARS-CoV-2 antigen from clinical specimensEBioMedicineJuly 1, 2021Renuse S, Vanderboom PM, Maus AD, Kemp JV, Gurtner KM, Madugundu AK, Chavan S, Peterson JA, Madden BJ, Mangalaparthi KK, et al.(Catalog# 11205D, Carlsbad, CA). Trypsin/Lys-C Mix (Catalog# V5071) and Rapid Digestion kit (Catalog# VA1060) was purchased from Promega (Madison, WI). Recombinant SARS-CoV-2 nucleocapsid protein was purchased

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Rapid Digestion Kit---Trypsin

Rapid Digestion Kit---Trypsin from Promega

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Rapid Digestion Kit---Trypsin from Promega

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