Fig 1: DC development in bone marrow cultures.(A) Representative FACS plots of WT and Pin1-null bone marrow-derived Mac1- cDC generated by culturing with FL. Cells were previously gated as B220-Mac1− (“Mac1−”) and cDC are identified as MHCIIhiCD11chi cells. The frequency of Mac1− cDC corresponding to the populations gated in the FACS plots on the left are graphed on the right as percentage of total cells (n = 16). (B) Representative FACS plots of WT and Pin1-null bone marrow-derived Mac1+ cDC generated by culturing with either FL (n = 16) or GM-CSF (n = 11). FL-generated cells were previously gated as B220-Mac1+, and GM-CSF-generated cells were previously gated as B220-Mac1+GR1-. In the plots shown, cDC are identified as MHCIIhiCD11chi cells. The frequencies of Mac1+ cDC gated on the left are depicted in the graph on the right as the percentage of total cells. (C) Representative overlaid histograms comparing CD11c expression in WT and Pin1-null B220-Mac1+ cells generated from FL and GM-CSF bone marrow cultures. (D) Representative FACS plots of WT and Pin1-null bone marrow-derived pDC and macrophage (mΦ). Cells were not previously gated. pDC are identified as PDCA1+CD11cint cells and macrophages are identified as F4/80+Mac1+ cells. The frequencies of WT and Pin1-null bone marrow-derived pDC (n = 16) and macrophages (n = 6) are shown in the graph on the right as percentage of total cells.