Human IL2Ra / CD25 Protein (His Tag) 10165-H08H from Sino Biological, Inc.

Product Specs
ItemHuman IL2Ra / CD25 Protein (His Tag)
CompanySino Biological, Inc.
Price $248.00Supplier Page View Company Product Page
Catalog Number10165-H08H
Size100 µg
Conjugate/TagC-His
Purity> 97 % as determined by SDS-PAGE. > 90 % as determined by SEC-HPLC
TypeRecombinant protein
Molecular WeightThe recombinant human IL2Rα consists of 203 amino acids and has a predicted molecular mass of 23.3 kDa. In SDS-PAGE under reducing conditions, the apparent molecular mass of rhIL2Rα is approximately 44 kDa due to glycosylation
SpeciesHuman
Molecule NameIL2RA
Source/Expression SystemHEK293 Cells
NCBI Full Gene Nameinterleukin 2 receptor subunit alpha
NCBI Gene AliasesCD25, IDDM10, IL2R, IMD41, TCGFR, p55
Activity1.Measured by its ability to inhibit IL2-induced proliferation of M07e cells. The ED50 for this effect is 0.2-1.2 µg/mL in the presence of 5 ng/mL of recombinant human IL2(Routinely tested). 2.Loaded Recombinant Human CD25/IL-2R alpha Protein, His Tag (Cat. No. 10165-H08H) on His1K Biosensor, can bind Recombinant Human IL-2 Protein, no Tag (Cat. No. 11848-HNAE2) with an affinity constant of 44.8 nM as determined in BLI assay (Sartorius Octet RED384) (QC tested). 3.Recombinant Human IL2Ra / CD25 Protein (His Tag) (Cat: 10165-H08H) captured on CM5 chip via Anti-His antibody, can bind Recombinant Human IL2 Protein, Low Endotoxin (Cat: 11848-HNAH1-E) with an affinity constant of 22.270 nM as determined in an SPR assay (Biacore T200) (Routinely tested)
FormatLyophilized from sterile PBS, pH 7.4

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Sino Biological, Inc.

37 Industrial Blvd

Suite B

Paoli, PA 19301

United States

Phone: 215-583-7898
Fax: 267-657-0217

Email: marketing@sinobiological.com

Company Profile

Website: sinobiological.com

(1) Targeting of specific neuronal types in the non-human primate brain by using a murine CD25-specific recombinant immunotoxinScientific ReportsFebruary 11, 2026Tomoko Kobayashi, Shigeki Kato, Shingo Kimura, Masateru Sugawara, Kanichiro Ihara, Tatsuhiko Ozawa, Ken-ichi Inoue, Masahiko Takada, Masanori Onda, Kazuto Kobayashito trap secreted IgG. The chip was incubated with 10 µg/mL hCD25/His-tag (Sino Biological Inc., Cat#10165-H08H) for 30 min as a blocking step, and then with 10 µg/mL biotinylated mCD25/His-tag for 30 min, followed

(2) SHR-1916: A Novel PEGylated Interleukin-2 Analogue with Altered Cellular Selectivity and Improved Pharmacokinetic Profiles for Cancer ImmunotherapyDrug Des Devel TherFebruary 24, 2025Xianglin Kong, Yuan Lin, Chao Ouyang, Hao Chen, Xiangdong Gaoamine coupling procedure (Biacore® protocol). The human CD25/IL-2Rα with a His-tag (Sino Biological, 10165-H08H) or CD122/IL-2Rβ with a His-tag (Sino Biological, 10696-H08B) was then immobilized. Analytes were prepared

(3) An antibody against human interleukin-2 showing minimal binding to neonatal Fc receptor: Potent anti-tumor activity and reduced systemic toxicity in miceEuropean journal of pharmacologyJanuary 15, 2025Lv Y, Chen J, Wang C, Jin Z, Lou J.

(4) SHR-1916: A Novel PEGylated Interleukin-2 Analogue with Altered Cellular Selectivity and Improved Pharmacokinetic Profiles for Cancer ImmunotherapyDrug design, development and therapyJanuary 1, 2025Kong X, Lin Y, Ouyang C, Chen H, Gao X.

(5) A single-agent fusion of human IL-2 and anti-IL-2 antibody that selectively expands regulatory T cellsCommun BiolMarch 9, 2024Yuan Lin, Xue Wang, Yuhao Qin, Chengpan Wang, Tang Zhou, Long Zhang, Lu Su, Wenming Ren, Cheng Liao(GE Healthcare) were used to make anti-His tag chip. The his-tagged human IL-2Rα (Sino Biological, 10165-H08H) or IL-2Rβγ (ACROBiosystems, ILG-H5283) was injected over the chip surface and captured as ligand. The

(6) Antitumor immunity induced by antibody-based natural killer cell engager therapeutics armed with not-alpha IL-2 variantCell reports. MedicineOctober 18, 2022Demaria O, Gauthier L, Vetizou M, Blanchard Alvarez A, Vagne C, Habif G, Batista L, Baron W, Belaïd N, Girard-Madoux M, et al.Binding studies were performed by capturing His-tagged antigens (CD25, CD122 or CD132) (Sino Biological, 10165-H08H, 10696-H08B and 10555-H08B, respectively) on the chip surface at 15 μg/mL. ANKET were diluted to 162.5

(7) Two novel human anti-CD25 antibodies with antitumor activity inversely related to their affinity and in vitro activityScientific reportsNovember 25, 2021Song D, Liu X, Dong C, Wang Q, Sha C, Liu C, Ning Z, Han J, Liu H, Zong M, et al.Positive clones were obtained and sequenced. ELISA-based binding assay for scFvs Human CD25 protein (10165-H08H, Sino Biological) was coated on high binding ELISA plates with 0.2 μg/mL at 4 °C overnight, and then

(8) Selection of DNA aptamers against Mycobacterium tuberculosis Ag85A, and its application in a graphene oxide-based fluorometric assayMikrochimica actaDecember 5, 2017Ansari N, Ghazvini K, Ramezani M, Shahdordizadeh M, Yazdian-Robati R, Abnous K, Taghdisi SM.

(9) Design, isolation and evaluation of the binding efficiency of a DNA aptamer against interleukin 2 receptor alpha, in vitroInternational immunopharmacologyDecember 1, 2017Shahdordizadeh M, Taghdisi SM, Sankian M, Ramezani M, Abnous K.

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Human IL2Ra / CD25 Protein (His Tag)

Human IL2Ra / CD25 Protein (His Tag) from Sino Biological, Inc.

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Sino Biological, Inc.

Citations (9)

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Human IL2Ra / CD25 Protein (His Tag) from Sino Biological, Inc.

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