Cynomolgus FCGRT & B2M Heterodimer Protein CT031-C08H from Sino Biological, Inc.

Description

A DNA sequence encoding the extracellular domain (Met 1-Ser 297) of cynomolgus FCGRT (Q8SPV9) was fused with a polyhistidine tag at the C-terminus, constructed the plasmid 1; A DNA sequence encoding the cynomolgus B2M (Q8SPW0) (Met 1-Met 119) constructed the plasmid 2. The two plasmids were co-expressed and the FCGRT/B2M heterodimer was purified

Product Specs
ItemCynomolgus FCGRT & B2M Heterodimer Protein
CompanySino Biological, Inc.
Price $348.00Supplier Page View Company Product Page
Catalog NumberCT031-C08H
Conjugate/TagC-His
Purity≥ 95 % as determined by SDS-PAGE. ≥ 90 % as determined by SEC-HPLC. ≥ 90 % as determined by SEC-MALS(Routinely tested)
TypeRecombinant protein
Molecular WeightThe recombinant heterodimer of cynomolgus FCGRT&B2M comprises 384 (285+99) amino acids and has a calculated molecular mass of 43.5 (31.9+ 11.6) KDa. The apparent molecular mass of cynomolgus FCGRT&B2M heterodimer is approximately 35 & 12 KDa respectively in SDS-PAGE The molecular weight of this protein is around 40-60 kDa verified by SEC-MALS (Routinely tested)
SpeciesCynomolgus
Molecule NameFCGRT & B2M
Source/Expression SystemHEK293 Cells
NCBI Gene AliasesFcrn
Activity1.Loaded Cynomolgus FcRn recombinant protein (Cat. CT031-C08H) on His1K Biosensor, can bind IgG1 Fc ( Cat. 10702-HNAC) with an affinity constant of 0.032 µM as determined in a BLI assay (Sartorius Octet Red384)(Routinely tested). 2.Captured Cynomolgus FcRn recombinant protein (Cat. CT031-C08H) on Anti-His Chip can bind Anti-VEGF (Research Grade Bevacizumab Biosimilar) (IgG1) with an affinity constant of 0.09 µM as determined in an SPR assay (Biacore T200)(Routinely tested). 3.Captured Cynomolgus FcRn recombinant protein (Cat. CT031-C08H) on Anti-His Chip can bind Anti-PD1(Research grade Nivolumab biosimilar) (IgG4) with an affinity constant of 0.18 µM as determined in an SPR assay (Biacore T200)(Routinely tested). 4.Recombinant Cynomolgus FCGRT & B2M Heterodimer Protein (Cat: CT031-C08H) captured on CM5 chip via Anti-His antibody, can bind anti-ERBB2(Research grade trastuz*mab biosimilar) with an affinity constant of 1.290 µM as determined in an SPR assay (Biacore 8K) (Routinely tested)
FormatLyophilized from sterile PBS, pH 7.4
Size100 µg