Rat IL-23 (IL23A & IL12B Heterodimer) Protein CT045-R08H from Sino Biological, Inc.

Description

A DNA sequence encoding the rat IL23A (NP_569094.1) (Met1-Ala196) was fused with a polyhistidine tag at the C-terminus, constructed the plasmid 1; A DNA sequence encoding the rat IL12B (EDM04146.1) (Met1-Ser335) was expressed with a polyhistidine tag at the C-terminus, constructed the plasmid 2. The two plasmids were co-expressed and the rat IL23A & the rat IL12B heterodimer was purified

Product Specs
ItemRat IL-23 (IL23A & IL12B Heterodimer) Protein
CompanySino Biological, Inc.
Price Supplier Page View Company Product Page
Catalog NumberCT045-R08H
Activity1.Measured by its ability to induce IL17 secretion by mouse splenocytes. The ED50 for this effect is 0.5-3ng/mL. 2.Loaded Recombinant Rat IL-23 Receptor Protein, hFc Tag (Cat. No. 80395-R02H) on proA Biosensor, can bind Recombinant Rat IL-23 Protein, His Tag (Cat. No. CT045-R08H) with an affinity constant of 32.700 nM as determined in BLI assay (Sartorius Octet RED384) (QC tested)
FormatLyophilized from sterile PBS, pH 7.4
Size20 µg
Source/Expression SystemHEK293 Cells
Molecule NameIL12B & IL23A
SpeciesRat
Molecular WeightThe recombinant rat IL23A & the rat IL12B comprises 510 (186+324) amino acids and has a calculated molecular mass of 58.2 (21+37.2) KDa. The apparent molecular mass of the rat IL23A & the rat IL12B heterodimer is approximately 47.08, 43.29, 27.67 and 24.75 KDa respectively in SDS-PAGE
TypeRecombinant protein
Purity> 90 % as determined by SDS-PAGE
Conjugate/TagC-His