SARS-CoV Spike/S1 Protein (S1 Subunit, His Tag) 40150-V08B1 from Sino Biological, Inc.

Product Specs
ItemSARS-CoV Spike/S1 Protein (S1 Subunit, His Tag)
CompanySino Biological, Inc.
Price $448.00Supplier Page View Company Product Page
Catalog Number40150-V08B1
FormatLyophilized from sterile 20 mM Tris, 300 mM NaCl, 10% glycerol, pH 7.5
ActivityMeasured by its binding ability in a functional ELISA. Immobilized human ACE2 protein (Fc tag)(10108-H05H) at 2µg/mL (100µL/well) can bind SARS-CoV Spike/S1 Protein (S1 Subunit, His Tag)(40150-V08B1),the EC50 of SARS-CoV Spike/S1 Protein (S1 Subunit, His Tag)(40150-V08B1) is 400-850 ng/mL
Size100 µg
TypeRecombinant protein
Source/Expression SystemBaculovirus-Insect Cells
Molecule NameSpike
SpeciesSARS
Molecular WeightThe recombinant S1 subunit of SARS-CoV (isolate:WH20) spike comprises 665 amino acids and has a predicted molecular mass of 74.4 kDa. The apparent molecular mass of the protein is approximately 85.8 kDa in SDS-PAGE under reducing conditions
Purity> 90 % as determined by SDS-PAGE
Conjugate/TagC-His

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Sino Biological, Inc.

37 Industrial Blvd

Suite B

Paoli, PA 19301

United States

Phone: 215-583-7898
Fax: 267-657-0217

Email: marketing@sinobiological.com

Company Profile

Website: sinobiological.com

(1) Evaporation‐Enhanced Redox Cycling for Rapid Detection of Attomolar SARS‐CoV‐2 Virions Using Nanolithography‐Free Electrochemical DevicesAdvanced Materials TechnologiesMay 22, 2025Pouya Soltan Soltan Khamsi, Vinay Kammarchedu, Aida Ebrahimi(Sino Biological, China, Catalog#: 40601-V08H), SARS-CoV S1 protein (Sino Biological, China, Catalog#: 40150-V08B1), or MERS-CoV S1 protein (Sino Biological, China, Catalog#: 40069-V08H) as non-target viruses). Next,

(2) Systematic Study of Various Functionalization Steps for Ultrasensitive Detection of SARS-CoV-2 with Direct Laser-Functionalized Au-LIG Electrochemical SensorsACS applied materials & interfacesSeptember 18, 2024Brustoloni CJ, Soltan Khamsi P, Kammarchedu V, Ebrahimi A.Human coronavirus (HCoV-NL63) Spike/S1 Protein (S1 Subunit, HIS Tag) (CAS: 40600-V08H), SARS-CoV Spike/S1 Protein (S1 Subunit, His Tag) (CAS: 40150-V08B1), MERS-CoV Spike/S1 Protein (S1 Subunit, aa 1-725, His Tag) (CAS: 40069-V0BH) were purchased from Sino Biological

(3) Virus-like structures for combination antigen protein mRNA vaccinationNature nanotechnologyAugust 1, 2024Zhang J, Li Y, Zeng F, Mu C, Liu C, Wang L, Peng X, He L, Su Y, Li H, et al.sequentially incubated with a primary mouse anti-SARS-CoV-2 S antibody (Sino Biological, catalogue number 40150-V08B1) and an Alexa Fluor 647-conjugated goat anti-mouse IgG secondary antibody (Invitrogen). DCs were detected

(4) S6P mutation in Delta and Omicron variant spike protein significantly enhances the efficacy of mRNA COVID-19 vaccinesFrontiers in immunologyJanuary 1, 2024Bong YS, Brown D, Chung E, Ananthaswamy N, Chen R, Lewoczko E, Sabbers W, Patterson-Orazem AC, Dorsey Z, Zou Y, et al.(Sino Biological, Cat #40591-V08H15), Gamma (Sino Biological, Cat #40589-V08H26), Delta (Sino Biological, Cat #40150-V08B1), BA.1 (Sino Biological, Cat #40591-V08H41), and BA.2 (Sino Biological, Cat #40589-V08H28). Additionally,

(5) Utilizing noncatalytic ACE2 protein mutant as a competitive inhibitor to treat SARS-CoV-2 infectionFrontiers in immunologyJanuary 1, 2024Liu S, Chen H, Chen X, Luo N, Peraramelli S, Gong X, Zhang MJ, Ou L.9.6) in a 96-well microplate. The S1 proteins were purchased from Sino Biological (SARS-CoV-1 S1 cat# 40150-V08B1, SARS-CoV-2 S1 cat# 40591-V08H, MERS-CoV S1 protein cat#: 40069-V08H, Beijing, China). The microplate

(6) Wireless, battery-free, multifunctional integrated bioelectronics for respiratory pathogens monitoring and severity evaluationNature CommunicationsNovember 20, 2023Hu Li, Huarui Gong, Tsz Hung Wong, Jingkun Zhou, Yuqiong Wang, Long Lin, Ying Dou, Huiling Jia, Xingcan Huang, Zhan Gao, et al.spike protein (Sino Biological, Cat:40069-V08H), and SARS-CoV spike protein (Sino Biological, Cat: 40150-V08B1); Spike antibody 1 (Sino Biological; Cat: 40150-R007; Clone ID: 007; Dilution, 1:4), spike antibody

(7) Effects of spike proteins on angiotensin converting enzyme 2 (ACE2)Archives of biochemistry and biophysicsOctober 15, 2023Bejoy J, Williams CI, Cole HJ, Manzoor S, Davoodi P, Battaile JI, Kaushik A, Nikolaienko SI, Brelidze TI, Gychka SG, et al.HEK293 cells were purchased from Sino Biological Inc. (Wayne, PA, USA). Recombinant SARS-CoV Spike S1 (Cat# 40150-V08B1) produced in Baculovirusinsect cells was also purchased from Sino Biological. Recombinant SARS-CoV-2

(8) Secondary Structures of MERS-CoV, SARS-CoV, and SARS-CoV-2 Spike Proteins Revealed by Infrared Vibrational SpectroscopyInternational journal of molecular sciencesMay 31, 2023D'Arco A, Di Fabrizio M, Mancini T, Mosetti R, Macis S, Tranfo G, Della Ventura G, Marcelli A, Petrarca M, Lupi S.polyhistidine tag at the C terminus of MERS-CoV (Cat. 40069-V08B1, aa 725, purity > 90%), SARS-CoV (Cat. 40150-V08B1, aa 665, purity > 90%), and SARS-CoV-2 (Cat. 40591-V08B1, aa 681, purity > 90%) were purchased from

(9) Broadly neutralizing aptamers to SARS-CoV-2: A diverse panel of modified DNA antiviral agentsMolecular Therapy - Nucleic AcidsMarch 14, 2023Amy D. Gelinas, Tiong Kit Tan, Sai Liu, Javier G. Jaramillo, James Chadwick, Adam C. Harding, Chi Zhang, Brian E. Ream, Chelsea N. Chase, Matthew R. Otis, et al.domain (ECD) (SinoBiological, cat #40589-V08B1); spike S1 subunit SARS-CoV-1 (SinoBiological, cat #40150-V08B1); spike S2 ECD (SinoBiological cat #40590-V08B); spike S1 RBD (Creative Biomart, cat #Spike-190V); and

(10) Cyanovirin-N binds to select SARS-CoV-2 spike oligosaccharides outside of the receptor binding domain and blocks infection by SARS-CoV-2Proceedings of the National Academy of Sciences of the United States of AmericaMarch 7, 2023Muñoz-Basagoiti J, Monteiro FLL, Krumpe LRH, Armario-Najera V, Shenoy SR, Perez-Zsolt D, Westgarth HJ, Villorbina G, Bomfim LM, Raïch-Regué D, et al.protein produced in baculovirus-infected insect cells was sourced from Sino Biological (catalog no. 40150-V08B1). His-tagged HEK293-produced trimeric Spike (S1+S2) was purchased from BPS Bioscience catalog #100728.

(11) Maturation of SARS-CoV-2 Spike-specific memory B cells drives resilience to viral escapeiScienceJanuary 20, 2023Marzi R, Bassi J, Silacci-Fregni C, Bartha I, Muoio F, Culap K, Sprugasci N, Lombardo G, Saliba C, Cameroni E, et al.

(12) Adenosine deaminase augments SARS-CoV-2 specific cellular and humoral responses in aged mouse models of immunization and challengeFrontiers in immunologyJanuary 1, 2023Gary EN, Tursi NJ, Warner BM, Cuismano G, Connors J, Parzych EM, Griffin BD, Bell MR, Ali AR, Frase D, et al.(Purified Wuhan recombinant Spike RBD, Sino Biological, BDA, Beijing, RBD-His 40592-VNAH, FC2), S1 (S1-His 40150-V08B1), and S2 (S2-mfc 40590-V05B). S2-His 40590-V05B expressed in insect cells was used in serum antibody

(13) SARS-CoV-2 infections elicit higher levels of original antigenic sin antibodies compared with SARS-CoV-2 mRNA vaccinationsCell reportsOctober 18, 2022Anderson EM, Li SH, Awofolaju M, Eilola T, Goodwin E, Bolton MJ, Gouma S, Manzoni TB, Hicks P, Goel RR, et al.FL-S were purchased (Sino Biological, Wayne PA; cat. 40606-V08B, 40021-V08H, 40021-V08B, 40634-V08B, 40150-V08B1, 40150-V08B3, and 40605-V08B, respectively). ELISAs Antibodies reactive to SARS-CoV-2, OC43, HKU1,

(14) Analysis of Serologic Cross-Reactivity Between Common Human Coronaviruses and SARS-CoV-2 Using Coronavirus Antigen MicroarraybioRxivSeptember 16, 2022Saahir Khan et al.,Sino Biological,N-(AA306-527)-His-C,40150-V08B2 Coronavirus,SARS,SARS,S1,AAX16192.1,Insect Cells,Sino Biological,N-(AA1-667)-His-C,40150-V08B1 Coronavirus,SARS,SARS,NP,NP_828858.1,Insect Cells,Sino Biological,N-(AA1-422)-His-C,40143-V08B Coronavirus,SARS,SARS,PLpro,AAX16193.1,E. coli

(15) A non-invasive ultrasensitive diagnostic approach for COVID-19 infection using salivary label-free SERS fingerprinting and artificial intelligenceJournal of photochemistry and photobiology. B, BiologySeptember 1, 2022Karunakaran V, Joseph MM, Yadev I, Sharma H, Shamna K, Saurav S, Sreejith RP, Anand V, Beegum R, Regi David S, et al.of SARS-CoV-2 (Sino Biological, Inc., Cat no: 40591-V08H), SARS-CoV(Sino Biological, Inc., Cat No: 40150-V08B1), and MERS-CoV (Sino Biological, Inc., Cat no: 40069-V08B1) was employed. For the studies with COVID

(16) Preclinical Immunogenicity and Efficacy of a Multiple Antigen-Presenting System (MAPS(TM)) SARS-CoV-2 VaccineVaccinesJuly 3, 2022Cieslewicz B, Makrinos D, Burke H, Bree D, Haridas R, Tonkiss I, Bartsch Y, Alter G, Malley R, Besin G.UWashington, 35962), RBD Soluble, his-tag (UWashington, 35961), and SARS-CoV-1 (S1 Subunit) S-RBD (40150-V08B1, Sino Biological, Chester Brook, PA, USA) and left overnight at RT. Similarly, AffiniPure F(ab’)2 Fragment-specific

(17) Antibody Light Chains: Key to Increased Monoclonal Antibody Yields in Expi293 Cells?Antibodies (Basel)May 18, 2022Siqi Gong, Seijal Gautam, Joshua D. Coneglio, Hanna B. Scinto, Ruth M. Ruprechtinstead of capture antibody we coated 100 µL of 1 µg/mL gp120 (SHIV-1157ipd3N4) or SARS spike protein (40150-V08B1, SinoBiological, Beijing, China). Antigen-specific mAb concentration was determined with the corresponding

(18) Rapid and Quantitative In Vitro Evaluation of SARS-CoV-2 Neutralizing Antibodies and NanobodiesAnalytical chemistryMarch 15, 2022Wu W, Tan X, Zupancic J, Schardt JS, Desai AA, Smith MD, Zhang J, Xie L, Oo MK, Tessier PM, et al.SARS-CoV-2 (2019-nCoV) Spike RBD-His recombinant protein (40592-V08H), and SARS-CoV spike/S1 protein (S1 subunit, His tag, 40150-V08B1) were provided by Sino Biological. Recombinant SARS-CoV-2 Spike His protein (10549-CV), recombinant

(19) Boosting of cross-reactive antibodies to endemic coronaviruses by SARS-CoV-2 infection but not vaccination with stabilized spikeeLifeMarch 15, 2022Andrew R Crowley et al.protein",SARS-CoV-1 S,Sino Biological,40634-V08B, "Peptide, recombinant protein",SARS-CoV-1 S1,Sino Biological,40150-V08B1, "Peptide, recombinant protein",MERS S,Sino Biological,40069-V08B, "Peptide, recombinant protein",MERS

(20) Boosting of cross-reactive antibodies to endemic coronaviruses by SARS-CoV-2 infection but not vaccination with stabilized spikeElifeMarch 15, 2022Andrew R Crowley, Harini Natarajan, Andrew P Hederman, Carly A Bobak, Joshua A Weiner, Wendy Wieland-Alter, Jiwon Lee, Evan M Bloch, Aaron AR Tobian, Andrew D Redd, et al.protein",SARS-CoV-1 S,Sino Biological,40634-V08B, "Peptide, recombinant protein",SARS-CoV-1 S1,Sino Biological,40150-V08B1, "Peptide, recombinant protein",MERS S,Sino Biological,40069-V08B, "Peptide, recombinant protein",MERS

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SARS-CoV Spike/S1 Protein (S1 Subunit, His Tag)

SARS-CoV Spike/S1 Protein (S1 Subunit, His Tag) from Sino Biological, Inc.

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Sino Biological, Inc.

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SARS-CoV Spike/S1 Protein (S1 Subunit, His Tag) from Sino Biological, Inc.

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