Human IL1R2 / CD121b Protein (Fc Tag) 10111-H02H from Sino Biological, Inc.

Product Specs
ItemHuman IL1R2 / CD121b Protein (Fc Tag)
CompanySino Biological, Inc.
Price $298.00Supplier Page View Company Product Page
Catalog Number10111-H02H
Size100 µg
Conjugate/TagC-human IgG1-Fc
Purity≥ 90 % as determined by SDS-PAGE
TypeRecombinant protein
Molecular WeightThe recombinant human IL1R2/Fc is a disulfide-linked homodimer. The reduced monomer consists of 568 amino acids and has a predicted molecular mass of 64.5 kDa. As a result of glycosylation, the rh IL1R2/Fc monomer migrates as an approximately 70-75 kDa band in SDS-PAGE under reducing conditions
SpeciesHuman
Molecule NameIL1R2
Source/Expression SystemHEK293 Cells
NCBI Full Gene Nameinterleukin 1 receptor type 2
NCBI Gene AliasesCD121b, CDw121b, IL-1R-2, IL-1RT-2, IL-1RT2, IL1R2c, IL1RB
Activity1. Measured by its binding ability in a functional ELISA. Immobilized recombinant human IL1B at 2 µg/ml (100ul/well) can bind recombinant human IL1R2-Fc with a linear range of 6.25-400 ng/ml(QC tested). 2. Using the Octet RED System, the affinity constant (Kd) of IL1R2 Protein, Human, Recombinant (Fc Tag) (Cat. 10111-H02H) bound IL-1 beta Protein, Human, Recombinant (Cat. 10139-HNAE) was 6.0 nM(Routinely tested). 3. Measured by its ability to inhibit Interferon gamma secretion by human natural killer lymphoma NK-92 cells in the presence of 500 pg/mL IL1b. The EC50 for this effect is typically 0.1-0.8 µg/mL(Routinely tested). 4.Loaded Recombinant Human IL1R2 / CD121b Protein (Fc Tag) (Cat. No. 10111-H02H) on ProA Biosensor, can bind Recombinant Human IL-1 beta / IL1B Protein (Cat. No. 10139-HNAE) with an affinity constant of 6.0 nM as determined in BLI assay (Sartorius Octet RED384) (Routinely tested). 5.Recombinant Human IL1R2 / CD121b Protein (Fc Tag) (Cat: 10111-H02H) captured on CM5 chip via Anti-Human IgG Fc, can bind Recombinant Human IL-1 beta / IL1B Protein (Cat: 10139-HNAE) with an affinity constant of 4.720 nM as determined in an SPR assay (Biacore 8K) (Routinely tested)
FormatLyophilized from sterile PBS, pH 7.4