Western blot analysis of Cell lysate before purification using DYKDDDDK (FLAG) peptide|Western blot analysis of DYKDDDDK tagged recombinant protein flow through wash using DYKDDDDK (FLAG) peptide|Western blot analysis of crude lysate containing over-expressed DYKDDDK-tagged recombinant protein using DYKDDDDK (FLAG) peptide
Supplier Page from Biorbyt for DYKDDDDK (FLAG) peptide
Application Notes: The FLAG peptide was tested by SDS-PAGE. A working concentration of 500 µg/mL for triple FLAG peptide is typically used for competitive elution of FLAG containing proteins from anti-DYKDDDDK affinity gels (p/n 200-350-383). For use as a control in antibody-peptide competition assays (PCA) use the peptide at 1.0 µg per 1.0 µl of antibody/antiserum in per assay. See anti-DYKDDDDK FLAG affinity gel PROTOCOL for specific instructions for use.
Application Dilutions: IP: User Optimized - see PROTOCOL, WB: User Optimized as blocking peptide in PCA