Recombinant Human GP73 Protein, HEK293 Lysate 230-10092-100 from RayBiotech

Product Specs
ItemRecombinant Human GP73 Protein, HEK293 Lysate
CompanyRayBiotech
Price $150.00Supplier Page View Company Product Page
Catalog Number230-10092-100
Size100 µg
Conjugate/TagN-terminal 6x histidine tag
FormatLyophilized powder
Molecular WeightThe recombinant human GP73 has a calculated molecular mass of 42 kDa. Due to the abundant glycosylation, it migrates as an approximately major 60 kDa protein bands in Western blotting under DTT, beta-mercaptoethanol reducing conditions
SpeciesHuman
Source/Expression SystemHEK293 cells
ConcentrationDetermined by BCA protein assay kit (Thermo Scientific)
NCBI Full Gene Namegolgi membrane protein 1
NCBI Gene AliasesGOLPH2, bA379P1.3, C9orf155, PSEC0257, HEL46, GP73
ApplicationsWB, ELISA
Molecule NameGolgi Membrane Protein 1, Chromosome 9 Open Reading Frame 155, GOLPH2, HEL46, Golgi Phosphoprotein 2, PSEC0257, C9orf155, bA379P1.3, Golgi Membrane Protein GP73, Epididymis Luminal Protein 46, GP73, Golgi Protein 73-KD
PurityUnpurified cell lysate. HEK293 cells transfected with expression vectors harboring target gene were harvested and washed with PBS twice. The cell pastes were re-suspended with ice-cold PBS containing mammalian cell protease inhibitor cocktail and further lysed with freeze-thaw cycles. After clarifying with 20,000 g centrifugation at 4°C for 30 min, the lysate was aliquoted, lyophilized, and stored at -80°C immediately. Protein concentration was determined by BCA kit (Thermo Scientific, Inc.) using BSA as protein standard. The gene overexpression in lysate was confirmed by Western blotting using anti-His tag antibody and/or target-specific antibodies and the lysate derived from HEK293 cells transfected with the empty expression vector was used as a negative control