Recombinant Human SPARC (BM40) Protein, HEK293 Lysate 230-10064-100 from RayBiotech

Product Specs
ItemRecombinant Human SPARC (BM40) Protein, HEK293 Lysate
CompanyRayBiotech
Price $150.00Supplier Page View Company Product Page
Catalog Number230-10064-100
Size100 µg
Conjugate/TagN-terminal 8x histidine tag
FormatLyophilized powder
Molecular Weight35 kDa
SpeciesHuman
Source/Expression SystemHEK293 cells
ApplicationsWB, ELISA
Molecule NameSecreted protein, acidic, cysteine-rich (osteonectin)
PurityUnpurified cell lysate. HEK293 cells transfected with expression vectors harboring target gene were harvested and washed with PBS twice. The cell pastes were re-suspended with ice-cold PBS containing mammalian cell protease inhibitor cocktail and further lysed with freeze-thaw cycles. After clarifying with 20,000 g centrifugation at 4°C for 30 min, the lysate was aliquoted, lyophilized, and stored at -80°C immediately. Protein concentration was determined by BCA kit (Thermo Scientific, Inc.) using BSA as protein standard. The gene overexpression in lysate was confirmed by Western blotting using anti-His tag antibody and/or target-specific antibodies and the lysate derived from HEK293 cells transfected with the empty expression vector was used as a negative control
Research AreaAngiogenesis
NCBI Gene AliasesON, OI17, BM-40, ONT
NCBI Full Gene Namesecreted protein acidic and cysteine rich
ConcentrationDetermined by BCA protein assay kit (Thermo Scientific)