Chymotrypsin, Sequencing Grade V1062 from Promega

Product Specs
ItemChymotrypsin, Sequencing Grade
CompanyPromega
Price Supplier Page View Company Product Page
Catalog NumberV1062
Size100microg
FormatSequencing Grade
Conjugate/TagUnconjugated
TypeEnzyme
Molecule NameChymotrypsin

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Promega

2800 Woods Hollow Rd

Madison, WI 53711

United States

Phone: Tel: +1 (608) 274-4330
Fax: +1 (608) 277-2516

Email: custserv@promega.com
techserv@promega.com

Company Profile

Website: http://www.promega.com

(1) The phosphoproteomic landscape of the neurological manifestations in tuberous sclerosis complexActa NeuropatholMay 20, 2026Marie Girodengo, Simeon R. Mihaylov, Katarzyna Klonowska, Laura Mantoan Ritter, Helen R. Flynn, J. Mark Skehel, Elias Bou Farhat, Eleonora Aronica, Matthew White, David J. Kwiatkowski, et al.with 10 mM CaCl2. Each sample was then digested with 5.56 µg chymotrypsin (Sequencing grade, Promega, V1061) shaking at 25 °C overnight. Sample cleanup and TMT labelling After digestion, samples were quenched

(2) Enhanced Proteomics Analysis with a Novel Recombinant Chymotrypsin Analogue Engineered for High Cleavage SpecificityJournal of Proteome ResearchMay 1, 2026Kish R. Adoni, Jonathan E. Ditcham, Alba Katiria González Rivera, Georgina H. Charlton, Sergei V. Saveliev, Konstantinos Thalassinos, Riccardo Zenezini ChiozziPreparation For all experiments relating to the benchmarking of rChymoSelect against standard chymotrypsin (V1061, Promega), a standard human K562 intact extract (10 mg/mL of human cell K562 lysed in 6 M Urea, Promega

(3) Protocol for MR1-ligand cross-linking to discover metabolite antigens by proteomicsSTAR ProtocMarch 20, 2026Hannah Thomas, Garry Dolton, Lucia F. Cardo, Nicola Ternette, Andrew K. Sewell, Thierry Schmidlin11873580001 Acetic acid,Fisher Scientific,Cat# 219992500 "Chymotrypsin, Sequencing Grade",Promega,Cat# V1061 Triethylammonium bicaronate buffer (TEAB),Merck,Cat# T7408 Biotin,Merck,Cat# B4639 PBS,Thermo Scientific,Cat#

(4) Generalizable direct protein sequencing with InstaNexusMolecular & Cellular ProteomicsMarch 2, 2026Marco Reverenna, Maike Wennekers Nielsen, Darian Stephan Wolff, Jemma Daniel, Elpida Lytra, Suthimon Thumtecho, Pasquale D. Colaianni, Anne Ljungars, Andreas H. Laustsen, Erwin M. Schoof, et al.proteases: papain (Sigma-Aldrich, P5306-25MG), proteinase K (Promega, V3021), chymotrypsin (Promega, V1061), GluC (Promega, V1651), thermolysin (Promega, P1512), and elastase (Sigma-Aldrich, 324681-50UG). Thermolysin

(5) Protocol for identification of protein citrullination by immunoprecipitation followed by mass spectrometrySTAR ProtocolsMarch 1, 2026Alexandra Indeglia, Hsin-Yao Tang,Thermo Fisher Scientific,Cat # 21580 CaCl2,Sigma-Aldrich,Cat# 21115 "Chymotrypsin, Sequencing Grade",Promega,Cat# V1061 "cOmplete, Mini, EDTA-free Protease Inhibitor Cocktail",Sigma-Aldrich,Cat# 11836170001 DMSO (Dimethyl

(6) Cryo-EM structures of light chain fibrils from abdominal fat biopsies of multiple myeloma patientsNature CommunicationsFebruary 24, 2026Yuxuan Yao, Shun Yao, Yamei Xu, Qinyue Zhao, Xingyu Xiong, Kaien Liu, Yu Liu, Zhizhi Wang, Wenqing Xu, Cong Liu, et al.and digested in-gel using sequencing grade modified trypsin (Promega, V5111), chymotrypsin (Promega, V1061), Asp-N (Promega, V1621), proteinase K (Promega, V3021), a-lytic (New England Biolabs, P8113). Peptides

(7) Identification of Unknown Biological Toxin Proteins Using Mass Spectrometry: A Case Study on De Novo Sequencing of RicinToxins (Basel)November 18, 2025Yubo Song, Hao Wang, Junjie Wen, Jiale Xu, Siyu Zhu, Fuli Wang, Yongqian Zhangdigested using four proteases: Trypsin (Promega, V511A), Glu-C (Promega, V1651), Chymotrypsin (Promega, V1061), and Proteinase K (Promega, V3021). The sample was first loaded into a 10 kDa molecular weight cut-off

(8) Structure-based engineering of a nutrient acquisition protein enhances neutralizing antibodies and protection for the development of a gonococcal vaccinebioRxivNovember 11, 2025Natalie Y.T. Au, En Ze L. Zhong, Jamie E. Fegan, Epshita A. Islam, Stacey X. Xu, Dixon Ng, Megha Shah, Laura-lee Caruso, Christine C.L. Lai, Anthony B. Schryvers, et al.PBS to a concentration of 0.2 mg/mL for a total volume of 60 μL. Chymotrypsin (0.6 μg, Promega, cat. V1061) or trypsin (0.3 μg, Promega, cat. V5111) was added to protein and incubated at 37°C. 10 μL of each

(9) Molecular dynamics of the matrisome across sea anemone life historyElifeOctober 23, 2025Bruno Gideon Bergheim, Alison G Cole, Mandy Rettel, Frank Stein, Stefan Redl, Michael W Hess, Aissam Ikmi, Suat Özbek2007RRID:AB_3711173,Immunogold EM (1:150) "Chemical compound, drug","Chymotrypsin, sequencing grade",Promega,Cat# V1061,Mass spectrometry "Chemical compound, drug",Cysteine,Sigma-Aldrich,Cat# C7352,Egg dejellying "Chemical

(10) Molecular dynamics of the matrisome across sea anemone life historyeLifeOctober 23, 2025Bruno Gideon Bergheim, Alison G Cole, Mandy Rettel, Frank Stein, Stefan Redl, Michael W Hess, Aissam Ikmi, Suat Özbek2007RRID:AB_3711173,Immunogold EM (1:150) "Chemical compound, drug","Chymotrypsin, sequencing grade",Promega,Cat# V1061,Mass spectrometry "Chemical compound, drug",Cysteine,Sigma-Aldrich,Cat# C7352,Egg dejellying "Chemical

(11) MR1-ligand cross-linking identifies vitamin B6 metabolites as TCR-reactive antigensCell Reports MethodsAugust 18, 2025Thierry Schmidlin, Enas Behiry, Hannah Thomas, Garry Dolton, Fabio Marino, Samiul Hasan, Magdalena von Essen, Rose M. Gathungu, Barbara A. Steigenberger, Hayden Selvadurai, et al.Scientific,Cat# 219992500 Urea,Fisher Scientific,Cat# U15-3 "Chymotrypsin, Sequencing Grade",Promega,Cat# V1061 Triethylammoniumbicaronate buffer (TEAB),Merck,Cat# T7408 Biotin,Merck,Cat# B4639 PBS,Thermo Scientific,Cat#

(12) Production and cryo-electron microscopy structure of an internally tagged SARS-CoV-2 spike ecto-domain construct☆Journal of Structural Biology: XJune 1, 2025Suruchi Singh, Yi Liu, Meghan Burke, Vamseedhar Rayaprolu, Stephen E. Stein, S. Saif Hasancombinations of proteases, including Alpha lytic WT (Sigma, A6362. 1:100 wt/wt), Chymotrypsin (Promega, V1061. 1:10), Trypsin (Promega, V5111. 1:20)/Lys-C (Promega, VA1671. 1:20) and Trypsin (1:20)/Glu-C (Promega,

(13) Structural proteomics defines a sequential priming mechanism for the progesterone receptorNat CommunMay 12, 2025Matthew D. Mann, Min Wang, Josephine C. Ferreon, Phoebe S. Tsoi, Michael P. Suess, Antrix Jain, Anna Malovannaya, Roberto Vera Alvarez, Bruce D. Pascal, Raj Kumar, et al.(trypsin:protein) for 4 h at 37 °C, then digested overnight at room temperature using chymotrypsin (Promega, V1061) at a ratio of 1:150 (w/w) (chymotrypsin:protein). Peptides were acidified to a final concentration

(14) Molecular parameters governing antibody FcγR signaling and effector functions in the context of HIV envelopeCell reportsApril 22, 2025Bick MV, Puig E, Beauparlant D, Nedellec R, Burton I, Ardaghi K, Zalunardo TR, Bastidas R, Li X, Guenaga J, et al.(Cytiva 28932225) and separately digested overnight using trypsin (Promega VA900A), chymotrypsin (Promega V1062), or alpha lytic protease at a ratio of 1:15 (w/w). Peptides were dried and extracted using an Oasis

(15) Using Protein Painting Mass Spectrometry to Define Ligand Receptor Interaction Sites for Acetylcholine Binding ProteinBIO-PROTOCOLJanuary 20, 2025Alexandru Graur, Natalie Erickson, Nadine Kabbanitrypsin (Promega, catalog number: V5111) 12. Sequencing-grade chymotrypsin (Promega, catalog number: V1061) 13. DNase (Sigma-Aldrich, catalog number: DN25-1G) 14. Complete EDTA-free mini protease inhibitor (Thermo Fisher Scientific

(16) Using Protein Painting Mass Spectrometry to Define Ligand Receptor Interaction Sites for Acetylcholine Binding ProteinBio ProtocJanuary 20, 2025Alexandru Graur, Natalie Erickson, Nadine Kabbanitrypsin (Promega, catalog number: V5111) 12. Sequencing-grade chymotrypsin (Promega, catalog number: V1061) 13. DNase (Sigma-Aldrich, catalog number: DN25-1G) 14. Complete EDTA-free mini protease inhibitor (Thermo Fisher Scientific

(17) The viral serpin SPI-1 directly inhibits the host cell serine protease FAM111AJournal of Biological ChemistryJanuary 10, 2025Allison L. Welter, Sowmiya Palani, Yuka Machida, Matthew J. Schellenberg, Yuichi J. MachidaNaCl, 0.81 mM DTT). For assays, 1 μM FAM111A SPD, 1 μM GST-FAM111A-Strep, 1 nM chymotrypsin (Promega, #V1061), or 50 nM cathepsin G (Enzo Life Sciences, BML-SE283–0100) were used of each respective protease. Three

(18) Proteomic profiling reveals the significance of lipid metabolism in small cell lung cancer recurrence and metastasisJournal of Translational MedicineDecember 20, 2024Haohua Zhu, Huiyang Shi, Jingyu Lu, Kai Zhu, Lin Yang, Lei Guo, Le Tang, Yuankai Shi, Xingsheng Hu(Cytiva, P/N: 45152105050250, 65152105050250) for 15 min at room temperature, then trypsin (Promega, P/N: V1062) was added at 1:50 trypsin-to-protein mass ratio for 16 h at 37 ℃ for on beads digestion. After trypsin

(19) Efficient suppression of premature termination codons with alanine by engineered chimeric pyrrolysine tRNAsNucleic Acids ResearchDecember 11, 2024Aya Awawdeh, Alejandro Tapia, Sarah A Alshawi, Olabode Dawodu, Sarah A Gaier, Caitlin Specht, Jean-Denis Beaudoin, Jeffery M Tharp, Oscar Vargas-Rodriguezconcentration of 10 mM. Proteolysis was achieved using sequencing grade chymotrypsin (Promega Corporation P/N V1062) at an enzyme:protein ratio of 1:20 (w/w) and proceeded for 6 h at 37°C. The digestion was quenched

(20) Intra-channel bi-epitopic crosslinking unleashes ultrapotent antibodies targeting NaV1.7 for pain alleviationCell Reports MedicineNovember 19, 2024Yaning Zhang, Yanchao Ding, Ziyan Zeng, Rui Zhu, Peiyuan Zheng, Shilong Fan, Qingjuan Cao, Hang Chen, Weishuai Ren, Mengling Wu, et al.BR100826 ACDTB,"Focken, T. et al.34",N/A "Chymotrypsin, Sequencing Grade",Promega Corporation,Cat# V1061 PEI Max,Polysciences,Cat# 24765 A-803467,MedChemExpress,HY-11079 PF-05089771,Bide Pharmatech.,BD292811 ,, Critical

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Chymotrypsin, Sequencing Grade

Chymotrypsin, Sequencing Grade from Promega

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Promega

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Chymotrypsin, Sequencing Grade from Promega

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