Fig 1: Dynamics of leading process growth cone on the Sdc2-coated stripe in the presence of CSPG.(A) Experimental scheme. CSPG-rich Matrigel corresponds to 60% Matrigel/Ncan/L15 medium. (B–D) Representative super-resolution time-lapse images of ventricular–subventricular zone (V-SVZ)-derived cultured migrating neurons expressing Venus-CAAX (green) and DsRed (red), which label membranes and cytosol, respectively. While “minute-interval” imaging reveals the overall dynamics of the leading process growth cone (B), “second-interval” imaging visualizes the dynamics of fine cellular structures such as lamellipodia and filopodia. (C, D) Yellow, light-blue, and magenta arrows indicate formed, buried, and retracted filopodium, respectively. The structure and dynamics of lamellipodial and filopodial structures should be additionally analyzed by using actin probes such as GFP-actin or EGFP-UtrCH. Numbers indicate seconds from the first imaging frame (B–D). Scale bars, 5 μm (B–D). CSPG, chondroitin sulfate proteoglycan.