Fig 1: Protein function is retained following intravitreal Nuc1 delivery of protein. (A) Representative TUNEL-stained retinal cryosections from mice injected intraperitoneally with MNU. Quantitation of TUNEL-stained retinal cells for each group of mice shows that Nuc1 coinjected with XIAP confers significant (65.7%, P < 0.0001) protection against MNU-induced apoptosis (TUNEL-positive cells) relative to untreated MNU-injected mice, while MNU-injected mice intravitreally injected with XIAP alone showed no significant reduction in apoptosis (TUNEL-positive cells; 2.2%, P = 0.9902) relative to untreated MNU-injected mice. (B) Representative TUNEL-stained retinal cryosections from mice injected in the subretinal space with sodium hyaluronate (Sodium H), a model of retinal detachment. Quantitation of TUNEL-stained retinal cells shows that Nuc1 coinjected with XIAP confers a significant (60%, P = 0.0061) reduction in apoptosis (TUNEL-positive cells) relative to mice injected with Sodium H alone. Representative images are randomly selected sections obtained from n = 3 to 4 animals per group. Data are presented as mean ± SD and was analyzed using an unpaired t-test and the data were considered significant at P < 0.05.