Fig 1: Donor Trem1/3 deletion reduces the inflammatory signature in recipient neutrophils after mouse heart transplantation(A) UMAP of recipient neutrophils colored by cell state.(B) Heatmap of marker genes for recipient neutrophil cell states.(C) Dot plot of key marker genes by neutrophil cell state.(D) Composition plot for donor neutrophil cell states grouped by genotype.(E) Heatmap of differentially expressed genes between WT and Trem1/3-deficient mice.(F) Gene Ontology analysis in donor Macs grouped by genotype (hypergeometric test for adjusted p value).(G) Progeny NF-κB pathway enrichment score in neutrophils.(H and I) Progeny NF-κB pathway enrichment score violin plot in recipient (H) neutrophils and (I) monocytes/Macs grouped by genotype.(J) Ccr1 and Ccl3 expression violin plots by cell type.(K) Cell chat showing the Ccr1-Ccl3 interaction axis. Two hearts per group were pooled for single-cell analysis.For (B) and (E), significantly differentially expressed genes were identified with adjusted p < 0.05 and log2FC > 0.25 by Wilcoxon rank-sum test. CXCL, C-X-C motif chemokine ligand; IL-1b, interleukin 1 beta; Ccrl2, C-C chemokine receptor-like 2; Nfkbi, nuclear factor kB inhibitor; plac8, placenta-associated 8.