Fig 1: Cell migration profile of HaCaT cells. Media containing complete sera induced migration of HaCaT cells in a 24-well chamber plate assay. Cell migration activity was determined quantitatively using Image-J after GIEMSA'S AZUR EOSIN Methylene Blue-staining of transwell filter membrane. A: The following were added to the bottom wells of a 24-well Boyden chamber: No addition (None); Sema3A (10 nM); kCer (25 μM); His (1 μM); CCL16 (10 nM, His agonist for H4R); and CCL17 (10 nM, non-His agonist). B: Cell migration was quantitated as % of control (no addition). Data are shown as the means ± SD (n = 4). *P < 0.05, **P < 0.001 vs. vehicle-treated control.
Fig 2: Combined effect of Sema3A or kCer with His or CCL chemokines (CCL16 or CCL17) on cell migration. A: Migration of HaCaT cells stimulated by no addition (None), Sema3A (100 nM), or kCer (25 μM) plus His (1 μM), CCL16 (10 nM), or CCL17 (10 nM). B: Effect of 100 nM Sema3A on cell migration by no other addition (None), 1 μM His, 10 nM CCL16 or 10 nM CCL17. C: Effect of 100 μM kCer on cell migration by no other addition (None), 1 μM His, 10 nM CCL16 or 10 nM CCL17. Cell migration was quantitated as % of control (no addition).Data are shown as the means ± SD (n = 4). *P < 0.05.
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