Fig 1: HIV-1 Replication in Pre-treated MDMs (A,B), Post HIV-1-infected MDMs (C,D) and -MDMs infected with pre-incubated inoculum- (E,F) treated with SLPI and SERPINC1. (A,B) MDMs (1 × 106 cells/well) were pre-treated with (A) SLPI 1 µg/mL and 10 µg/mL or (B) SERPIN C1 0.05 µg/mL and 0.1 µg/mL concentration for 3 h then infected with HIV-1 BaL 5 ng/mL p24 units. After 2 h the virus was removed, and fresh culture media added with 1 µg/mL and 10 µg/mL SLPI or 0.05 µg/mL and 0.1 µg/mL SERPINC1 and cultured. (C,D) MDMs (1 × 106 cells/well) were infected with HIV-1 BaL 5 ng/mL p24 units. After 2 h, the virus was removed, and fresh culture media added with (C) SLPI 1 µg/mL and 10 µg/mL concentration or (D) SERPIN C1 0.05 µg/mL and 0.1 µg/mL and cultured the MDMs. (E,F) HIV-1 BaL (5 ng/mL p24 units) was incubated without or with (E) SLPI 1 µg/mL and 10 µg/mL or (F) SERPIN C1 0.05 µg/mL and 0.1 µg/mL at 37 °C for 2 h in PBS containing 0.1% BSA. After that, MDMs (5 × 105 cells/well) were infected with HIV-1 BaL and incubated at 37 °C for 2 h for virus adsorption washed with phosphate-buffered saline (PBS) and cultured in 2 mL fresh media at 37 °C. Culture supernatants were collected after 7 days post infection and HIV-1 replication quantitated by HIV-1 p24 ELISA. Culture supernatants were analyzed in triplicate. Results expressed as mean ± SEM. This data is representative of three independent donors. Asterisk (*) over the bars indicates significant difference with control, *** p < 0.0001; ** p < 0.001; * p ≤ 0.05 and ns p > 0.05. p-values were generated by one-way ANOVA with multiple comparisons.