Fig 1: Innate responses to SNV in Huh7 and A549 are specifically due to Vero E6-derived IFNλ.Total cellular RNA was isolated from Huh7 or A549 cells and used for ISG quantitation by qRT-PCR. (A) Huh7 were treated with SNV (50 µL of virus) or the indicated amount of recombinant human IL29 or IL28A for 3 h prior to MxA mRNA quantitation by qRT-PCR. (B) Huh7 were infected with SNV at an MOI = 1 (50 µL) with or without prior incubation with anti-IL29 or anti-IL28A antibodies at the indicated final concentrations for 1 h. Three hours post infection, ISG56 and MxA mRNA expression was measured by qRT-PCR. (C) SNV or IFNβ was preincubated with the indicated amount of anti-IFNβ antibody for 1 h. Virus or IFNβ was then added to Huh7 cells for 3 h and ISG56 and MxA was measured 3 h post exposure by qRT-PCR. (D) A549 were infected with SNV at an MOI = 1, with or without prior incubation with anti-IL29 blocking antibodies (10 µg/mL). Three hours post infection ISG56 and MxA levels were measured by qRT-PCR. Real-time qRT-PCR results for all experiments (parts A–D) are reported as the mean ± SEM from triplicate biological experiments.