Cultrex Rat Collagen I 3440-100-01 from R&D Systems, a Bio-Techne Brand

Product Specs
ItemCultrex Rat Collagen I
CompanyR&D Systems, a Bio-Techne Brand
Price $225.00Supplier Page View Company Product Page
Catalog Number3440-100-01
SpeciesRat
Molecule NameCollagen I
Size100 mg
ApplicationsBioactivity
FormatType I Collagen provided at 5 mg/ml (Sircol Assay). Sterility Testing: No bacterial or fungal growth detected after incubation at 37°C for 14 days following USP XXIV Chapter 71 sterility test. No mycoplasma contamination detected by PCR
StorageStorage Buffer: 20 mM Acetic Acid Storage/Stability: Product is stable for a minimum of 3 months if stored at 4 °C. Do Not Freeze
NCBI Full Gene Namecollagen type I alpha 1 chain
NCBI Gene AliasesCAFYD, EDSARTH1, EDSC, OI1, OI2, OI3, OI4

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R&D Systems, a Bio-Techne Brand

614 McKinley Place N.E.

Minneapolis, Minnesota 55413

United States

Phone: 1 (800) 343-7475
1 (612) 379-2956

Fax: 1 (612) 656-4400

Company Profile

Website: http://www.rndsystems.com

(1) Doxorubicin exposure leads to cardiac fibroblast dysregulation and worsens fibrotic remodeling in the pathological heart☆J Mol Cell Cardiol PlusApril 13, 2026Sukriti Bagchi, Amy Cai, Alina S. Bilal, Javier Gamez-Luque, Manuel Rosa-Garrido, Bethany Patton, Mrinalini Kala, Christopher C. Glembotski, Erik A. BlackwoodμL of CFB were aliquoted into a 1.5 mL Eppendorf tube to which 240 μL of Collagen (R&D Systems, cat# 3440–100-01) was added and mixed thoroughly, carefully avoiding bubbles. NaOH (12 μL) was rapidly added and mixed,

(2) Binding of human low-density lipoproteins to type-I collagen is synergistic with the pro-atherogenic lipoprotein remodelingBiochimica et Biophysica Acta (BBA) - Molecular Basis of DiseaseMarch 1, 2026Shobini Jayaraman, Kyeongseo Choi, Antonio Pérez, Inka Miñambres, Jose Luis Sánchez-Quesada, Olga Gurskyelastin (Elastin Products Company #AE17, from bovine neck ligament), collagen type-I (Biotechne R&D #3440–100-01, from rat tail tendon), collagen type-IV (Sigma #CC076, from human placenta), and collagen type-VI (Biotechne

(3) Targeting FGFR4 abrogates HNF1A-driven metastasis in pancreatic ductal adenocarcinomaMolecular cancerJuly 29, 2025Crawford KJ, Humphrey KS, Cortes E, Wang J, Kumarasamy V, Wan Y, Long MD, Feigin ME, Witkiewicz AK, Mann KM, et al.

(4) Cytochalasins Suppress 3D Migration of ECM-Embedded Tumoroids at Non-Toxic ConcentrationsInternational journal of molecular sciencesJuly 21, 2025Beslmüller K, van Megen LJA, Struik T, Batenburg D, Neubert E, Evers TMJ, Mashaghi A, Danen EHJ.

(5) Augment proteasome inhibitor efficacy activates CD8+ T cell-mediated antitumor immunity in breast cancerCell Rep MedJuly 15, 2025Dongyang Tang, Shiqi Lin, Jingbo Zhou, Josh Haipeng Lei, Fangyuan Shao, Heng Sun, Xiangpeng Chu, Ling Li, Lin He, Yunfeng Qiao, et al.HY-12488 Ammonium tetrathiomolybdate,Sigma Aldrich,Cat #: 323446 Rat Collagen I,R&D Systems,Cat #: 3440-100-01 Human EGF Recombinant Protein,Thermo Fisher Scientific,Cat #: PHG0311 Mouse IL-2 Protein,Medchemexpress,Cat

(6) Reduced nephrin tyrosine phosphorylation impairs podocyte force transmission and accelerates detachment in diseaseiScienceJune 20, 2025Casey R. Williamson, Claire E. Martin, J. Dinesh Kumar, Peihua Lu, Laura A. New, Alice Y. Wang, Nils M. Kronenberg, Malte C. Gather, Paul A. Reynolds, Nina Jonesproteins" ,, Recombinant mouse interferon gamma (IFN-γ),PeproTech,Cat #: 315-05 Cultrex rat collagen I,R&D Systems,Cat #: 3440-100-01 Cultrex mouse collagen IV,R&D Systems,Cat #: 3410-010-01 Teklad standard chow diet (14% fat),Inotiv,Cat

(7) The fibrin-derived peptide FX06 protects human pulmonary endothelial cells against the COVID-19-triggered cytokine stormFrontiers in ImmunologyJune 19, 2025Zhiran Wang, Dmitrii Lebedev, Simeng Li, Sudharshan Rao, Kevin Wu, Lorcan Doyle, Kieran Wynne, Alfonso Blanco, Margaritha M. Mysior, Jeremy C. Simpson, et al.Germany). On day 1, wells of the 96-well plate was first coated with a solution of 5 mg/mL Collagen I (cat#3440-100-01, R&D Systems, USA) in phosphate-buffered saline (PBS), and incubated for 2 h at 37°C. One-hundred µL

(8) Tight Spaces, Tighter Signals: Spatial Constraints as Drivers of Peripheral MyelinationCellsJune 18, 2025Luca Bartesaghi, Basilio Giangreco, Vanessa Chiappini, Maria Fernanda Veloz Castillo, Martina Monaco, Jean-Jaques Médard, Giovanna Gambarotta, Marco Agus, Corrado Calì(VWR International LLC, Radnor, PA, USA) pre-coated with 50 µg/mL ammoniated rat collagen (Cultrex 3440-100-01; R&D Systems, Minneapolis, MN, USA). For cultures in microfluidic chambers, 2 DRG per chamber were plated

(9) Protocol for differentiating primary human small airway epithelial cells at the air-liquid interfaceAmerican Journal of Physiology-Lung Cellular and Molecular PhysiologyJune 1, 2025Yu Par Aung Myo, Sarah V. Camus, Margaret A. T. Freeberg, Tytus Bernas, Divya Bande, Rebecca L. Heise, Thomas H. Thatcher, Patricia J. Simeany collagen coating and seeded onto ALI inserts coated with rat tail collagen 1 (R&D Systems, Cat# 3440–100-01) at passage 4 as previously described(7, 9). Our lab incubators were routinely set at 7% CO2 to accommodate

(10) Fibulin-4 and latent-transforming growth factor beta-binding protein-4 interactions with syndecan-2 and syndecan-3 are required for elastogenesisFASEB JApril 15, 2025Hana Hakami, Neha E. H. Dinesh, Valentin Nelea, Nathalie Lamarche-Vane, Sylvie Ricard-Blum, Dieter P. Reinhardttype I solution was used to generate disk‐shaped gels for contraction assays (Cultrex, R&D Systems; #3440‐100‐01). Confluent cells were trypsinized, washed with serum‐free DMEM medium, and resuspended in DMEM containing

(11) Exosome-loading miR-205: a two-pronged approach to ocular neovascularization therapyJ NanobiotechnologyJanuary 22, 2025Hui-Ying Zhang, Qiu-Yang Zhang, Qing Liu, Si-Guo Feng, Yan Ma, Feng-Sheng Wang, Yue Zhu, Jin Yao, Biao Yan20% FBS and 80% methocel. The collagen mix was prepared on ice including Collagen type I (1 mg/mL, 3440-100-01, R&D Systems, USA) and PBS in a 2:3 ratio. The collagen solution was combined with spheroid solution

(12) Epidermal growth factor receptor specific immunotherapy for SHH medulloblastoma tested in an in vitro blood-brain barrier-modelCancer treatment and research communicationsJanuary 1, 2025Krüger C, Paplomatas P, Kreuter N, Hellwig M, Eckhardt A, Conze C, Oliveira-Ferrer L, Tischendorf J, Thaden V, Schüller U, et al.

(13) Multicompartmentalized Microvascularized Tumor-on-a-Chip to Study Tumor-Stroma Interactions and Drug Resistance in Ovarian CancerCellular and Molecular BioengineeringOctober 1, 2024Simona Plesselova, Kristin Calar, Hailey Axemaker, Emma Sahly, Amrita Bhagia, Jessica L. Faragher, Darci M. Fink, Pilar de la Puentecells/ml of NF, NOF or CAF were seeded in a 3D human plasma matrix with 2 mg/ml Cultrex collagen I (3440-100-01, R&D Systems) in 1:1 ratio. Alternatively, the monocultures of each stromal cell type were explored

(14) Human organoids with an autologous tissue-resident immune compartmentNatureSeptember 1, 2024Recaldin T, Steinacher L, Gjeta B, Harter MF, Adam L, Kromer K, Mendes MP, Bellavista M, Nikolaev M, Lazzaroni G, et al.

(15) Generation of human iPSC-derived 3D bile duct within liver organoid by incorporating human iPSC-derived blood vesselNature communicationsAugust 28, 2024Carolina E, Kuse Y, Okumura A, Aoshima K, Tadokoro T, Matsumoto S, Kanai E, Okumura T, Kasai T, Yamabe S, et al.

(16) Dystrophin deficiency impairs cell junction formation during embryonic myogenesis from pluripotent stem cellsiScienceJuly 19, 2024Mozin E, Massouridès E, Mournetas V, Lièvre C, Bourdon A, Jackson DL, Packer JS, Seong J, Trapnell C, Le Guiner C, et al.Medium,amsbio,Cat# SKM02 Skeletal Muscle. Myotube Medium,Amsbio,Cat# SKM03 Cultrex Rat Collagen I,R&D Systems,Cat# 3440-100-01 Paraformaldehyde,Thermo Fisher,Cat# #28906 Triton 100X,Eurobio,Cat# GAUTTR001 BSA (immunofluorescence),Sigma-Aldrich,Cat#

(17) GRT-X Stimulates Dorsal Root Ganglia Axonal Growth in Culture via TSPO and Kv7.2/3 Potassium Channel ActivationInternational journal of molecular sciencesJuly 3, 2024El Chemali L, Boutary S, Liu S, Liu GJ, Middleton RJ, Banati RB, Bahrenberg G, Rupprecht R, Schumacher M, Massaad-Massade L.poly-L-lysine (PLL) (Sigma Aldrich Chimie Saint-Quentin-Fallavier, France) and collagen (R&D systems 3440-100-01; Minneapolis, MN, USA) (refer to Supplementary Material S1A for coating details). The coverslips were

(18) Functional diversity among cardiolipin binding sites on the mitochondrial ADP/ATP carrierThe EMBO JournalJune 5, 2024Nanami Senoo, Dinesh K Chinthapalli, Matthew G Baile, Vinaya K Golla, Bodhisattwa Saha, Abraham O Oluwole, Oluwaseun B Ogunbona, James A Saba, Teona Munteanu, Yllka Valdez, et al.cells (Invitrogen R71007) were grown on a plate coated by Cultrex Rat Collagen I (50 µg/ml, R&D systems 3440-100-01) at 37 °C in 5% CO2 in high glucose-containing DMEM (Corning 10-013-CM) supplemented with 10% FBS (CPS

(19) Multicompartmentalized microvascularized tumor-on-a-chip to study tumor-stroma interactions and drug resistance in ovarian cancerbioRxiv : the preprint server for biologyJune 2, 2024Plesselova S, Calar K, Axemaker H, Sahly E, de la Puente P.

(20) Targeting the HSP47-collagen axis inhibits brain metastasis by reversing M2 microglial polarization and restoring anti-tumor immunityCell reports. MedicineMay 21, 2024Wang L, Li C, Zhan H, Li S, Zeng K, Xu C, Zou Y, Xie Y, Zhan Z, Yin S, et al.,Bio X Cell,Cat# BE0361; RRID: AB_2927503 PLX3397,MolDiets,Cat# M18072603 Cultrex rCollagen I,R&D Systems,Cat# 3440-100-01 Collagenase 1,Worthington,Cat# LS004197 ACK Lysis Buffer,LEAGENE,Cat# CS0001 Dnase 1,Solarbio,Cat#

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Cultrex Rat Collagen I from R&D Systems, a Bio-Techne Brand

R&D Systems, a Bio-Techne Brand

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Cultrex Rat Collagen I from R&D Systems, a Bio-Techne Brand

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