Fig 1: Characterization and Specificity of the EL-NE Monoclonal Antibody. (A): Competitive ELISA showing inhibition of free peptide (GGPGFGPGVV), elongated peptide (GGPGFGPGVVG), nonsense peptide (VGAGVPGLGV) and nonsense screening peptide (Biotin-KK- VGAGVPGLGV). (B): EL-NE fragment levels after 48 hours in vitro incubation of intact human elastin separately in buffer (ELN), elastin incubated in the presence of NE, MMP-2, MMP-7, MMP-9 and MMP-12. NE incubated for 48 hours separately in buffer was added as control. Abbreviations: ELN, elastin; NE, Neutrophil elastase; MMP, matrix metalloproteinase.