Klotho Protein 5334-KL-025 from R&D Systems, a Bio-Techne Brand

Product Specs
ItemKlotho Protein
CompanyR&D Systems, a Bio-Techne Brand
Price $408.00Supplier Page View Company Product Page
Catalog Number5334-KL-025
FormatSupplied as a 0.2 µm filtered solution in MES, Glycerol and EDTA
Size25 ug
Molecule NameKlotho
NCBI Full Gene Nameklotho
Purity>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining
Molecular WeightObserved: 120-130 kDa, reducing conditions
Theoretical: 109.8 kDa
ApplicationsBioactivity
Conjugate/TagUnconjugated
NCBI Gene AliasesHFTC3, KLA
Source/Expression SystemNS0-derived Recombinant Human Klotho (aa 34-981) Protein, CF
StorageUse a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after opening
SpeciesHuman

Specifications/Features

Available conjugates: Sizes Available: 25 ug

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R&D Systems, a Bio-Techne Brand

614 McKinley Place N.E.

Minneapolis, Minnesota 55413

United States

Phone: 1 (800) 343-7475
1 (612) 379-2956

Fax: 1 (612) 656-4400

Company Profile

Website: http://www.rndsystems.com

(1) Klotho protein alleviates heart ischemia/reperfusion injury and oxidative stress through regulation of the NOS/MMP pathwayScientific reportsSeptember 2, 2025Olejnik A, Krzywonos-Zawadzka A, Sambor I, Mysiak A, Bil-Lula I.in vitro chemical IRI with and without the administration of 1 µg/mL of Recombinant Human Klotho protein (5334-KL-025, R&D Systems, Minneapolis, USA), as described in the experimental protocol previously reported15. The

(2) IGF-1 Signaling Modulates Oxidative Metabolism and Stress Resistance in ARPE-19 Cells Through PKM2 FunctionInternational journal of molecular sciencesDecember 20, 2024Ravera S, Puddu A, Bertola N, Verzola D, Russo E, Maggi D, Panfoli I.

(3) A New Approach to the Quantification of Fibroblast Growth Factor 23-An Array Surface Plasmon Resonance Imaging BiosensorInternational journal of molecular sciencesOctober 18, 2023Tokarzewicz A, Ołdak Ł, Młynarczyk G, Klekotka U, Gorodkiewicz E.

(4) Klotho prevents transforming growth factor-β2-induced senescent-like morphological changes in the retinal pigment epitheliumCell death & diseaseMay 20, 2023Jang HY, Kim SJ, Park KS, Kim JH.TGF-β2 (# 7346-B2-005, R&D Systems) with or without 10 fmoles of α-klotho. Recombinant human α-klotho (# 5334-KL-025, R&D Systems, Minneapolis, MN, USA) was dissolved in PBS solution containing 50% glycerol, 0.1 mM EDTA,

(5) Klotho ameliorates angiotension-II-induced endothelial senescence via restoration of autophagy by inhibiting Wnt3a/GSK-3β/mTOR signaling: A potential mechanism involved in prognostic performance of Klotho in coronary atherosclerotic diseaseMechanisms of ageing and developmentApril 1, 2023Mao Q, Deng M, Zhao J, Zhou D, Tong W, Xu S, Zhao X.

(6) The Anti-Aging Hormone Klotho Promotes Retinal Pigment Epithelium Cell Viability and Metabolism by Activating the AMPK/PGC-1α PathwayAntioxidants (Basel)February 5, 2023Shuyan Zhou, Jacob Hum, Kaan Taskintuna, Stephanie Olaya, Jeremy Steinman, Junfeng Ma, Nady Golestanehh. Fresh HBSS containing 10 nM recombinant human Klotho protein (R&D Systems, Minneapolis, MN, USA, 5334-KL-025) was added. Cells were harvested at 0 min, 15 min, 30 min, and 1 h after Klotho treatment and processed

(7) Klotho Protein Decreases MMP-Mediated Degradation of Contractile Proteins during Ischaemia/Reperfusion Injury to the CardiomyocytesInternational journal of molecular sciencesDecember 7, 2022Olejnik A, Krzywonos-Zawadzka A, Banaszkiewicz M, Bil-Lula I.[7,16], in the absence or presence of 1 µg/mL [14,16] of recombinant human Klotho protein (R&D Systems, 5334-KL-025). 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid (HEPES) buffer (5.5 mmol/L HEPES, 63.7 mmol/L CaCl2,

(8) The Klotho protein supports redox balance and metabolic functions of cardiomyocytes during ischemia/reperfusion injuryCardiology JournalSeptember 30, 2022Agnieszka Olejnik, Marta Banaszkiewicz, Anna Krzywonos-Zawadzka, Iwona Bil-Lulain the absence or presence of 1 μg/mL [7, 12] of the Recombinant Human Klotho Protein (R&D Systems, 5334-KL-025). The aerobic stabilization and reperfusion were performed in 4-(2-hydroxyethyl)-1-piperazineethanesulfonic

(9) The Klotho protein supports redox balance and metabolic functions of cardiomyocytes during ischemia/reperfusion injuryCardiol JSeptember 30, 2022Agnieszka Olejnik, Marta Banaszkiewicz, Anna Krzywonos-Zawadzka, Iwona Bil-Lulain the absence or presence of 1 μg/mL [7, 12] of the Recombinant Human Klotho Protein (R&D Systems, 5334-KL-025). The aerobic stabilization and reperfusion were performed in 4-(2-hydroxyethyl)-1-piperazineethanesulfonic

(10) Renal Klotho and inorganic phosphate are extrinsic factors that antagonistically regulate hematopoietic stem cell maintenanceCell reportsFebruary 15, 2022Du C, Wang X, Wu Y, Liao W, Xiong J, Zhu Y, Liu C, Han W, Wang Y, Han S, et al.

(11) Klotho Ameliorates Vascular Calcification via Promoting AutophagyOxidative medicine and cellular longevityJanuary 1, 2022Li L, Liu W, Mao Q, Zhou D, Ai K, Zheng W, Zhang J, Huang L, Xu S, Zhao X.[16]. Furthermore, KO mice and their WT littermates received 0.02 mg/kg Klotho recombinant protein (KL; 5334-KL-025, R&D Systems, USA) or saline every 48 h for 2 weeks [17]. All treatments were administered through intraperitoneal injection

(12) Soluble αKlotho downregulates Orai1-mediated store-operated Ca²⁺ entry via PI3K-dependent signalingPflugers Archiv : European journal of physiologyApril 1, 2021Kim JH, Park EY, Hwang KH, Park KS, Choi SJ, Cha SK.Sigma-Aldrich (St Louis, MO, USA). Recombinant αKlotho (human) protein was provided from R&D Systems (cat no. 5334-KL-025, Minneapolis, MN, USA). Non-targeting control oligonucleotides (cat. n. SN-1003) and small interfering

(13) Protective effects of klotho on palmitate-induced podocyte injury in diabetic nephropathyPloS oneJanuary 1, 2021Suk Kang J, Son SS, Lee JH, Lee SW, Jeong AR, Lee ES, Cha SK, Chung CH, Lee EY.

(14) A novel antibody for the detection of alternatively spliced secreted KLOTHO isoform in human plasmaPloS oneJanuary 1, 2021Jadhav S, Tripathi S, Chandrekar A, Waikar SS, Hsiao LL.secreted KL (secKL), protein, aa 34–549 (ab84072, abcam) or recombinant soluble KL (sKL) protein, 34–981 (5334-KL-025, R&D systems) in 100 μl carbonate-bicarbonate buffer pH9.5 (C3041-50CAP, Sigma -Aldrich) and incubated

(15) Klotho retards renal fibrosis through targeting mitochondrial dysfunction and cellular senescence in renal tubular cellsPhysiological reportsJanuary 1, 2021Miao J, Huang J, Luo C, Ye H, Ling X, Wu Q, Shen W, Zhou L.

(16) Salt causes aging-associated hypertension via vascular Wnt5a under Klotho deficiencyThe Journal of clinical investigationAugust 3, 2020Kawarazaki W, Mizuno R, Nishimoto M, Ayuzawa N, Hirohama D, Ueda K, Kawakami-Mori F, Oba S, Marumo T, Fujita T.

(17) Soluble Klotho, a biomarker and therapeutic strategy to reduce bronchopulmonary dysplasia and pulmonary hypertension in preterm infantsScientific reportsJuly 23, 2020Batlahally S, Franklin A, Damianos A, Huang J, Chen P, Sharma M, Duara J, Keerthy D, Zambrano R, Shehadeh LA, et al.starved for 48 h. Serum-starved HPAECs were treated with varying doses of recombinant human Klotho (Cat # 5334-KL-025; R&D Systems; 0.01–0.5 µg/ml) and cultured in normoxic (21% O2, 5% CO2) or hyperoxic (95% O2, 5% CO2)

(18) Klotho protein contributes to cardioprotection during ischaemia/reperfusion injuryJournal of cellular and molecular medicineJune 1, 2020Olejnik A, Krzywonos-Zawadzka A, Banaszkiewicz M, Bil-Lula I.reperfusion, 16 in the presence and absence of 1 µg/mL 17 of Recombinant Human Klotho Protein (R&D Systems, 5334‐KL‐025). The aerobic stabilization and reperfusion was performed in 4‐(2‐hydroxyethyl)‐1‐piperazineethanesulfonic

(19) Mechanistic Roles of Matrilin-2 and Klotho in Modulating the Inflammatory Activity of Human Aortic Valve CellsCellsFebruary 7, 2020The E, Yao Q, Zhang P, Zhai Y, Ao L, Fullerton DA, Meng X.

(20) Urinary Extracellular Vesicles Carrying Klotho Improve the Recovery of Renal Function in an Acute Tubular Injury ModelMolecular therapy : the journal of the American Society of Gene TherapyFebruary 5, 2020Grange C, Papadimitriou E, Dimuccio V, Pastorino C, Molina J, O'Kelly R, Niedernhofer LJ, Robbins PD, Camussi G, Bussolati B.

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7/10/2019Recombinant Human Klotho Protein I Bound by anti-Klotho Ab Anonymous ReviewerReagent is suitable to screen for novel binders.

Klotho Protein from R&D Systems, a Bio-Techne Brand

Specifications/Features

R&D Systems, a Bio-Techne Brand

Citations (36)

Reviews

Klotho Protein from R&D Systems, a Bio-Techne Brand

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