Recombinant Mouse IFN-γ (carrier-free) 575308 from BioLegend

Product Specs
ItemRecombinant Mouse IFN-γ (carrier-free)
CompanyBioLegend
Price $703.00Supplier Page View Company Product Page
Catalog Number575308
Format0.22 µm filtered protein solution is in 20 mM HEPES, 150 mM NaCl, 5 mM TCEP, pH 7.2
Size500 µg
ApplicationsBioassay
Gene Nameinterferon gamma
NCBI Gene AliasesIFN-g, If2f, Ifg
NCBI Full Gene Nameinterferon gamma
Molecule NameIFN-gamma
SpeciesMouse
Concentration10 and 25 µg sizes are bottled at 100 µg/mL. 100 µg size and larger sizes are lot-specific and bottled at the concentration indicated on the vial. To obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool

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BioLegend

8999 BioLegend Way

San Diego, California 92121

United States

Phone: 1-877-246-5343 Toll-Free, USA & Canada
1-858-768-5800 International
Fax: 1-877-455-9587

Company Profile

Website: biolegend.com

(1) In vitro generated macrophages reflect the immunosuppressive phenotype of in vivo glioblastoma-associated macrophagesOncoImmunologyDecember 31, 2026Matthew Nazzaro, Elaine R. Mardis, Mykyta Artomov, Ella Juenger, Justin Lyberger, Mark Damante, Gregory Behbehani, Susobhan Sarkar, Prajwal Rajappamedia supplemented with 0.5 µg/mL GM-CSF (Biolegend 576304). On day 6, 20 ng/mL of IFNγ (Biolegend 575304) and 50 ng/mL LPS (Invitrogen 00-4976-93) were added to the media for 24 h. For M2 control macrophages,

(2) Synthesis of 4-Aminoindole-Containing Cephalosporin Prodrugs and Their Biological Activity against Mycobacterium tuberculosisACS Infectious DiseasesJuly 10, 2026Sangmi Oh, Helena I. M. Boshoff, Jose Santinni O. Roma, Aidan Boving, Danielle M. Weiner, April Walker, Laura E. Via, Clifton E. Barrycells in half of the 24w plates were activated for 8 h at 37 °C/5% CO2 with 20 ng/L IFN-γ (Biolegend #575302) in MCM, followed by washing (twice) with DMEM and overnight stimulation at 37 °C/5% CO2 with 100 ng/mL

(3) Coordinated Interferon-γ/Tumor Necrosis Factor axis Drives Selective Loss of Activated Enteric Glia in Inflammatory Bowel DiseasesCellular and Molecular Gastroenterology and HepatologyJune 12, 2026Marvin Bubeck, Klara A. Penkert, Heidi Limberger, Miguel González Acera, Christina Plattner, Svenja Ziegler, Anoohya Muppirala, Patrycja Forster, Manuel Jakob, Reyes Gamez-Belmonte, et al.Cytokine,Concentration,Catalogue #,Company Ms TNF recombinant,50 ng/mL,12343016,ImmunoTools GmbH Ms IFN-γ recombinant,50 ng/mL,575306,"BioLegend, Inc." Ms IL1β recombinant,50 ng/mL,12340013,ImmunoTools GmbH Ms IL17A recombinant,50 ng/mL,12340174,ImmunoTools

(4) IFN-γ PET imaging stratifies and predicts response to CD137 agonism in a syngeneic colorectal tumor modelJ Immunother CancerJune 3, 2026Alexander John Deck, Allen-Dexter Saliganan, Savannah Lane, Hyejeong Jang, Kang Chen, Seongho Kim, Nerissa T. Violabb-ifng) according to manufacturer’s instructions. Briefly, anti-IFN-γ was incubated with IFN-γ (BioLegend 575306) at an antibody:protein ratio of 10:1 overnight at 37°C in 96 well plates containing 105 B16-Blue cells

(5) Activated T cell extracellular vesicle DNA transfer enhances antigen presentation and anti-tumor immunityCancer CellMay 11, 2026Mengying Hu, Di-Ao Liu, Inbal Wortzel, Paul Collier, Theodore M. Nelson, Jonathan Foox, Guojie Zhong, Gabriel Tobias, Tetsuhiko Asao, Linda Bojmar, et al.replenished with 1 mL of fresh medium with the addition of PBS, 5 ng mL−1 of recombinant IFN-γ (Biolegend, 575302), 2μM of SAR-20347 (MedChemExpress, HY-100895), mouse ATEVs or NTEVs (containing 5 μg mL−1 of EVDNA),

(6) Whole-genome doubling drives immune evasion by silencing antigen presentationCancer CellMay 7, 2026Pierre Foidart, Zheqi Li, Xinran Cai, Marco Seehawer, Daniel D. Brown, Amatullah Tawawalla, Pilar Baldominos, Salma Parvin, Jun Nishida, Ernesto Rojas-Jimenez, et al.human interferon gamma,Sigma-Aldrich,Cat#I17001 Recombinant Mouse IFN-γ (carrier-free),Biolegend,Cat# 575306 doxorubicin,Sigma-Aldrich,Cta#D1515-10MG Cytochalasin D,Sigma,Cat#C2618-200UL Hoechst 33342,Life Technologies

(7) The long noncoding RNA lnc13 restrains inflammatory responses to maintain oral tolerance to glutenNature ImmunologyApril 30, 2026Ruxiao Yang-Fischer, Alyssa Shearer, Felipe Batista Leão, Izei Pascual-Gonzalez, Arnold Han, Ainara Castellanos-Rubio, Govind Bhagat, Sankar Ghoshheat-inactivated FBS and treated with or without 100 ng ml−1 of TNF (PeproTech 315-01 A), IFN-γ (BioLegend 575302), IL-6 (PeproTech 216-16) or soluble IL-15. Flow cytometry The following conjugated antibodies were

(8) SPEN loss drives extra-follicular diffuse large B cell lymphoma with female-specific lethality and therapeutic vulnerabilitiesCancer DiscovApril 21, 2026Benedikt Pelzer, Cem Meydan, Isaac M Spiegel, Ioannis Karagiannidis, Min Xia, Matt Teater, Emma M Welter, Zowie E Searcy, Laura K Hilton, Darko Barisic, et al.Jackson, 115–006-020; human: 10μg/mL; Jackson, 109–006-129) and IFNg (mouse: 0.001μg/mL; BioLegend, 575302; human: 0.01μg/mL; PeproTec, 300–02). After 48 and 72 hrs (mouse) or 72 hrs (human) cells were stained

(9) Small Subset, Big Impact: Regulatory Function of γδ T Cells in ArteriogenesisCellsApril 17, 2026Kira-Sofie Wimmer, Carolin Baur, Matthias Kübler, Christoph Arnholdt, Konda Kumaraswami, Franziska Heim, Katharina Elbs, Michael Reha Rohrmoser, Daphne Merkus, Elisabeth DeindlTreatment Recombinant interferon γ (IFNγ) substitution was performed by intravenous injection of 1 µg IFNγ (575308, BioLegend; equivalent to 40 µg/kg body weight, diluted in 100 µL sterile saline) via the tail vein.

(10) A compartmentalized inflammatory landscape and macrophage plasticity regulate Tet2 +/− -mediated clonal hematopoiesisBloodApril 16, 2026Kevin Lee, Cih-Li Hong, Wimeth Dissanayake, Gulzada Kulzhanova, Alexander Noel Pfeffer, Haiyin Li, Senthil Sivakumar, Zi Yin, Emily R. Quarato, Lauren Benoodt, et al.R&D Systems). Animal treatments Interferon gamma (IFN-γ, 100 μg/mouse, intraperitoneally [IP], catalog no. 575306; BioLegend) was administered 18 hours before intravital imaging of major histocompatibility complex

(11) Elevated IgG levels induce an M2‑to‑M1 phenotypic shift in mucosal macrophages and restrict the growth of invasive sphenoid sinus pituitary adenomasMol Med RepApril 14, 2026Xingbo Li, Zhuowei Lei, Sihan Li, Linpeng Xu, Quanji Wang, Qian Jiang, Zihan Wang, Huaqiu Zhang, Yimin Huang, Ting Leiinduced by lipopolysaccharide (LPS; 100 ng/ml; L2880; Sigma-Aldrich; Merck KGaA) and IFN-γ (20 ng/ml; 575302; BioLegend, Inc.), and M2 polarization was induced by 20 ng/ml IL-4 (PHC0045; Thermo Fisher Scientific,

(12) Sustained interferon exposure creates a hyper-metastatic subset of melanoma cellsbioRxivApril 5, 2026Mark H. Mannino, Tao Wei, Daniel Cassidy, Benjamin Knight, Devon L. Moose, Adrienne Shannon, Elena Piskounova, Zhiyu Zhao, Sean J. Morrisonculture, M-CSF was removed and the macrophages were stimulated for 24 hours with 20 ng/ml IFN-γ (Biolegend 575306) and 40 ng/ml lipopolysaccharide (Sigma L2880). Approximately 24 hours later, flow cytometrically-isolated

(13) Intraperitoneal CF33-hNIS combined with PD-L1 blockade eradicates gastric cancer peritoneal metastases and prevents recurrence via durable T cell memoryJ Immunother CancerApril 1, 2026Annie Yang, Zhifang Zhang, Anthony K Park, Shyambabu Chaurasiya, Sang-In Kim, Jianming Lu, Yoya Vashi, Jennifer Cillis, Hannah Valencia, Courtney Chen, et al.treated with PBS, IFNβ1 (20 ng/mL, Biolegend, Catalog#581306), or IFNγ (20 ng/mL, Biolegend, Catalog#575306) for 18 hours. Cells were harvested, blocked with anti-mouse CD16/32 antibody (Biolegend, Catalog#156604,

(14) Microglial HDAC3 inhibits the migration of CD8-positive T cell in the development of EAE by restraining the expression of CCL5Cell & BioscienceMarch 26, 2026Na Che, Yang Zhang, Bo Wang, Hongxing Wu, Zhiqiong Mao, Zengqiang Yuan, Xiangxi Kong, Yajin Liao(#S0C3035-1 × 96T) was purchased from STARTER. Mouse TNF-α ELISA kit (#430904) and Recombinant mouse IFN-γ (#575304) were purchased from BioLegend. RGFP966 (#S7229), Anti-mouse CD8α-InVivo (#A2102, the anti-CD8a neutralizing

(15) Non-cognate CD8 Binding to MHC I Promotes Positive Selection of an MHC-E Restricted CD8 T Cell PopulationEur J ImmunolMarch 12, 2026Xiaokun Yang, Melissa A. Colden, Rachel Coombs, Kathya Arana, Dan Tran, Michael Manoharan Valerio, Ellen A. Robey, Laurent Coscoy37°C, 5% CO2. DC 2.4 cells were then treated with 50 ng/mL Recombinant Mouse IFN‐γ (Biolegend, Cat. no: 575302) for 24 h, then Recombinant Mouse IFN‐γ was washed off. Single‐cell suspensions of QFLTg β2mKO thymi

(16) MERTK inhibition cooperates with immunomodulatory cyclophosphamide to induce CXCL9⁺ monocyte-macrophage programming and durable anti-tumor immunity in triple negative breast cancerbioRxivFebruary 6, 2026Alex J. Smith, Zachary Schrank, Nan Guan, Diego A. Pedroza, Sebastian Calderon, Xueying Yuan, Na Zhao, Zoe Gabriel, Yang Gao, Charlotte Rivas, et al.treatment groups included: DMSO untreated, 1μg/mL LPS (Millipore Sigma, L2880) + 50ng/mL IFNγ (BioLegend, 575306), 10ng/mL IL-4 (BioLegend 574304), 10ng/mL IL-13 (Fisher Scientific, 413ML005), 2μM MRX-2843, or MRX-2843

(17) In vivo detection of immune responses via cytokine activity labelingCellFebruary 5, 2026Guangqing Lu, Shanshan Zhang, Mengyang Feng, Eunha Kim, Daniel Cho, Jae Hyun Kim, Hannah Caris, Lev Silberstein, Gloria B. Choi, Jun R. HuhRecombinant Mouse IFN-α (carrier-free),BioLegend,Cat# 752802 Recombinant Mouse IFN-γ (carrier-free),BioLegend,Cat# 575308 Recombinant Mouse IL 1α (carrier free),BioLegend,Cat# 575002 Recombinant Mouse IL-1β (carrier-free),BioLegend,Cat#

(18) Aromatic microbial metabolite hippuric acid enhances inflammatory responses in macrophages via TLR-MyD88 signaling and lipid remodelingCell ReportsJanuary 27, 2026Gauri Mirji, Sajad Ahmad Bhat, Mohamed El El Sayed, Sarah Kim Reiser, Siva Pushpa Gavara, Ying Ye, Taito Miyamoto, Wujuan Zhang, Peter Vogel, Joel Cassel, et al.M1-like phenotype by LPS (100 ng/mL; Sigma, L2880) or LPS (100 ng/mL) plus IFNγ (100 ng/mL; BioLegend, 575306), or to M2-like phenotype by IL-4 (10 ng/mL; BioLegend, 574304) stimulation for 8hr. Macrophages were

(19) In vitro generated macrophages reflect the immunosuppressive phenotype of in vivo glioblastoma-associated macrophagesOncoimmunologyJanuary 25, 2026Matthew Nazzaro, Elaine R. Mardis, Mykyta Artomov, Ella Juenger, Justin Lyberger, Mark Damante, Gregory Behbehani, Susobhan Sarkar, Prajwal Rajappamedia supplemented with 0.5 µg/mL GM-CSF (Biolegend 576304). On day 6, 20 ng/mL of IFNγ (Biolegend 575304) and 50 ng/mL LPS (Invitrogen 00-4976-93) were added to the media for 24 h. For M2 control macrophages,

(20) Salmonella sensitizes macrophages for context-dependent anti-inflammatory responsesbioRxivJanuary 17, 2026Gloria Alvarado, Keara Laneadded at this time, either 100 ng/mL LPS (Fisher Scientific #NC1198692) and 5 ng/mL IFN-γ (BioLegend #575302) for M1 ligands, or 25 ng/mL IL-4 (BioLegend #574302) and 25 ng/mL IL-10 (BioLegend #575802) for M2

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Recombinant Mouse IFN-γ (carrier-free) from BioLegend

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Recombinant Mouse IFN-γ (carrier-free) from BioLegend

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