Recombinant Human IFN-γ (carrier-free) 570204 from BioLegend

Product Specs
ItemRecombinant Human IFN-γ (carrier-free)
CompanyBioLegend
Price $147.00Supplier Page View Company Product Page
Catalog Number570204
Format0.22 µm filtered protein solution is in 10mM Sodium succinate, 4% Mannitol, pH 5.0
Size25 µg
ApplicationsBioassay
Gene Nameinterferon, gamma
NCBI Gene AliasesIFG, IFI, IMD69
NCBI Full Gene Nameinterferon gamma
Molecule NameIFN-gamma
Concentration10 and 25 µg sizes are bottled at 100 µg/mL. 100 µg size and larger sizes are lot-specific and bottled at the concentration indicated on the vial. To obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool
SpeciesHuman

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BioLegend

8999 BioLegend Way

San Diego, California 92121

United States

Phone: 1-877-246-5343 Toll-Free, USA & Canada
1-858-768-5800 International
Fax: 1-877-455-9587

Company Profile

Website: biolegend.com

(1) B cell CD19 is transferred between immune cells in mice and humansNat CommunJuly 29, 2026Jasmin Ochs, Pia Schweineberg, Jacqueline Thode, Alica Blenkle, Leila Husseini, Matthias Klein, Tobias Bopp, Patrick Schindler, Friedemann Paul, Martin S. Weberhuman serum, 50 U/ml penicillin, 50 µg/ml streptomycin), containing 10 ng/ml human IFN-γ (BioLegend; #570206). Human iPSC-derived microglia generation Human induced pluripotent stem cells (hiPSC) UMGi130-A clone

(2) Active learning-guided mechanistic modeling reveals context-specific regulators of CXCL9 expression in pancreatic cancer cellsMolecular Systems BiologyJune 4, 2026Bi-rong Wang, Maaruthy Yelleswarapu, Lucie Descamps, Federica EduatiActivity Assay Kit,Thermo Fisher Scientific,E13184 Recombinant Human IFNγ (carrier-free),BioLegend,570204 Recombinant Human TNFα (carrier-free),BioLegend,592702 Recombinant Human IFNα2 (carrier-free),BioLegend,570102 Momelotinib

(3) Selective degradation of TBK1 uncovers mechanistic insights into blocking ccRCC progressionCell Chemical BiologyApril 16, 2026Chengheng Liao, Siying Lyu, Rebecca L. Johnson, Tiantian Shang, Xiaoyu Wang, Nina Gildor, Shina Li, Noelle S. Williams, Lindsey I. James, Lianxin Hu, et al.EL,MedChem Express,HY-Y1890 Human TNF-α protein,MedChem Express,HY-P7058 Recombinant Human IFN-γ,BioLegend,570206 Critical commercial assays,Critical commercial assays,Critical commercial assays MTS Assay Kit (Cell

(4) Inflammatory arthritis irAE may represent a unique autoimmune disease primarily driven by T cells but likely not autoantibodiesSci AdvApril 3, 2026Xingxing Zhu, Yue Yu, Shiju Chen, Hannah E. Langenfeld, Yanfeng Li, Panwen Wang, Ying Li, Chantal McCabe, Andrew C. Hanson, Brenna E. Sharp, et al.anti–human Ig (M + G + A) (2.5 μg/ml), rhIL-21 (10 ng/ml), rhIFN-γ (20 ng/ml; BioLegend, catalog no. 570204). Pembrolizumab (100 μg/ml; Keytruda) or isotype control IgG4 (Bio X Cell, catalog no. CP147) was added

(5) IFN-γ-induced trained immunity enhances killing of priority pathogens in healthy and genetically vulnerable individualsJCI insightMarch 23, 2026Murphy DM, Batten I, O'Farrell A, Carlile SR, O'Rourke SA, Court C, Morris B, Leisching G, Jameson G, Connolly SA, et al.serum (Sigma-Aldrich, H4522). Monocytes were left untrained or trained with IFN-γ (10 ng/mL; BioLegend, 570206) for 24 hours. Cells were washed with PBS, to remove IFN-γ, and incubated for 5 days to allow monocytes

(6) ChemR23 prevents phenotypic switching of vascular smooth muscle cells into macrophage-like foam cells in atherosclerosisCardiovascular ResearchMarch 16, 2026Bryce R Evans, Julia Schulz, Vasiliki Triantafyllidou, Anais Yerly, Manovriti Thakur, Nico Angliker, Mark Siegrist, Yvonne Jansen, Yi Yan, Sanne L Maas, et al.Cranbury, NJ, USA, #300-01A-100UG) or 100 ng/mL interferon (IFN)-γ (BioLegend, San Diego, CA, USA #570206) or were treated with the vehicles dimethyl sulfoxide (DMSO) or Milli-Q H2O to serve as controls, for

(7) Impact of Cellular Senescence on LCN2 Expression in Salivary Gland Epithelial Cells and Oral KeratinocytesBioFactorsFebruary 18, 2026Yosuke Shikama, Kayo Yoshida, Yuka Shikamapolarized into M1 macrophages by a 24‐h incubation with 20 ng/mL of recombinant human IFN‐γ protein (#570204, BioLegend) and 10 pg/mL of Escherichia coli lipopolysaccharide (LPS) (#tlrl‐eblps, InvivoGen), the

(8) Method for generation and ex vivo expansion of genetically edited mouse TregsJournal of Leukocyte BiologyDecember 1, 2025Yosef Gilad, Adam M Dean, Yan Xia, Sang Jun Han, David M Lonard, Bert W O'Malleyproliferation experiments, 103 cells were plated on a 12-well plate with or without IFNγ (100 ng/mL; BioLegend; 570206). Media changes with freshly supplemented IFNγ were performed every 3 to 4 d. Upon reaching confluence,

(9) Single-Cell Immune Profiling Reveals Neutrophils Promote Myasthenia Gravis Exacerbation Through BAFF SecretionAdvanced science (Weinheim, Baden-Wurttemberg, Germany)December 1, 2025Zhang Z, Dong J, Qiu M, Bai J, Cheng X, Song X.serum. Experimental conditions included treatment with recombinant human IFN‐γ (Biolegend, USA; Cat. 570206) stimulation at graded concentrations. Following incubation, BAFF levels in culture supernatants were

(10) Type I IFNs Decrease SARS-CoV-2 Replication in Human Cardiomyocytes and Increase Cytokine Production in MacrophagesJ Clin ImmunolOctober 21, 2025Verónica Durán, Eirini Nikolouli, Shambhabi Chatterjee, Bibiana Costa, Andreas Pavlou, Annett Ziegler, Jennifer Becker, Kira Baumann, Matthias Bruhn, Kathrin Haake, et al.96-well flat bottom plate. Where indicated, cells were treated with the specified concentrations of IFNγ (570206, Biolegend) or IFNα (592706, Biolegend). CMs were infected with SARS-CoV-2 at a multiplicity of infection

(11) Alphavirus replicons encoding IFN-γ enhance cancer virotherapy by overcoming macrophage-mediated suppressioniScienceOctober 17, 2025Laura Horvathova, Priscilla Kinderman, Thijs Janzen, Baukje Nynke Hoogeboom, Franz J. Weissing, Nadine van Montfoort, Toos Daemen, Darshak K. Bhatt(M-CSF),Biolegend,572903 Interleukin-4 (IL-4),Biolegend,574004 Interferon-gamma (IFN-γ),Biolegend,570204 Lipopolysaccharides (LPS) from E. coli O128:B12,Sigma Aldrich,L2755 LIberase TL,Sigma Aldrich,05401020001 Mouse

(12) Macrophages expressing chimeric cytokine receptors have an inflammatory phenotype and antitumoral activity upon IL-10 or TGFβ stimulationJ Immunother CancerOctober 15, 2025Sabrina Traxel, Florian Schmidt, Corina Beerli, Dinh-Van Vuong, Roberto F Speck, Simon BredlIn some experiments, macrophages were stimulated for an additional 48 hours with IFN-γ (BioLegend, 570204), IL-10 (BioLegend, 571004) or TGFβ1 (PeproTech, 100–21) at indicated concentrations. Plasmid construction To

(13) Loss of the tumor suppressor PTEN activates cell-intrinsic interferon signaling to drive immune resistancebioRxivSeptember 25, 2025Yubao Wang, Cherubin Manokaran, Jiajia Chen, Hao Gu, David Liu, Philippe Bousso, Jean J Zhao, Thomas M RobertsRecombinant Human IFN-α2 (carrier-free) and recombinant Human IFN-γ (carrier-free) were purchased from Biolegend (#592702 for IFN-α, #570206 for IFN-γ). Recombinant Human IFN-β was purchased from PeproTech (#300-02bc-5UG). Antibodies Antibodies

(14) Cell Line-Dependent Internalization, Persistence, and Immunomodulatory Effects of Staphylococcus aureus in Triple-Negative Breast CancerCancers (Basel)September 9, 2025Sima Kianpour Rad, Runhao Li, Kenny K. L. Yeo, Clare Cooksley, Gohar Shaghayegh, Sarah Vreugde, Fangmeinuo Wu, Yoko Tomita, Timothy J. Price, Wendy V. Ingman, et al.Methods 2.1. Reagents Interferon gamma (IFN-γ) was purchased from BioLegend (San Diego, CA, USA, Cat# 570204). Purified S. aureus lipoteichoic acid (LTA) (Cat# tlrl-pslta) and the synthetic TLR2 agonist Pam3CSK4

(15) Ectopic HLA-II Expression in ESCC: Exploration of Its Relationship with Neoantigen Burden and Patient SurvivalCellsSeptember 8, 2025Yupei Ji, Zhizhong Wang, Zhenguo Cheng, Shuangshuang Lu, Nick R. Lemoine, Renato Baleeiro, Louisa S. Chard Dunmall, Yaohe Wangmycoplasma-free. 2.4. IFN-γ Stimulation All ESCC cell lines were stimulated with 50 ng/mL human recombinant IFN-γ (#570206; BioLegend, San Diego, CA, USA) in T75 culture flasks for 48 h [21]. After incubation, the cells were

(16) SARS-CoV-2 Nsp1 Is a Major Suppressor of HLA Class I and Class II ExpressionVirusesAugust 5, 2025Ivo Schirmeister, Nicolas Eckert, Sebastian Weigang, Jonas Fuchs, Lisa Kern, Georg Kochs, Anne Haleniusplates for 2 h at 37 °C with 1 mL OptiMEM containing the virus with or without IFNγ (ca 700 U/mL, Cat#570208, Biolegend, San Diego, CA, USA). Then, cells were washed with PBS, and 2 mL DMEM containing 2% FCS,

(17) EZH2 loss during metabolic stress drives restoration of MHC Class I machinery in melanomaiScienceJune 20, 2025Jacob L. Edmondson, Megan R. Reed, Daniel Fil, Billie Heflin, Alyson McKinnon, Michael A. Bauer, Lauren C. Morehead, Nathan L. Avaritt, Mary Phillips, Sean D. Taverna, et al.IL-2,PeproTech,Cat# 212-12 Recombinant human IL-2,PeproTech,Cat# 200-D2 Mouse IFN-g,BioLegend,Cat# 570204 Human IFN-g,BioLegend,Cat# 575304 Cyclohexamide,Millipore,Cat# C7698-1G ,, Critical commercial assays,Critical

(18) Targeted apoptotic immune modulator for the treatment of metastatic EGFR-positive solid tumorsProceedings of the National Academy of SciencesJune 3, 2025Derrick Broka, Shoshana Klein, Alexei Shir, Babette Schade, Meera Saxena, Athanasia Dasargyri, Anita Jarzebinska, Caroline De Feyter, Davor Bajic, David Colecchia, et al.TAR001 for 48 h. As a positive control, M1 polarization medium containing IFNγ (BioLegend, Cat no. 570204) and LPS (Sigma-Aldrich/Merck, Cat no. L2880) was used. The change in the expression of M1 (CD80, CD86)

(19) Syndecan-3 positively regulates the pro-inflammatory function of macrophagesCell Mol Life SciApril 7, 2025So Young Lee, Endika Prieto-Fernández, Leire Egia-Mendikute, Asier Antoñana-Vildosola, Paloma Velasco-Beltrán, Alexandre Bosch, Borja Jimenez-Lasheras, Ander de Blas, Jone Etxaniz-Diaz de Durana, Eunate Valdaliso-Díez, et al.differentiated THP-1 macrophages were polarized adding recombinant human IFNγ (100 ng/mL, Biolegend Cat. #: 570206), IL-4 (20 ng/ml, Miltenyi Biotec S.L Cat. #: 130-112-411), or IL-13 (20 ng/ml, Miltenyi Biotec S.L

(20) Systems immunology analysis of human immune organoids identifies host-specific correlates of protection to different influenza vaccinesCell Stem CellApril 3, 2025Zachary W. Wagoner, Timothy B. Yates, Jenny E. Hernandez-Davies, Suhas Sureshchandra, Erika M. Joloya, Aarti Jain, Rafael de Assis, Jenna M. Kastenschmidt, Andrew M. Sorn, Mahina Tabassum Mitul, et al.Recombinant Human IL-12 (p70) (carrier-free),Biolegend,Cat # 573004 Recombinant Human IFN-γ (carrier-free),Biolegend,Cat # 570204 Influenza vaccine (LAIV FluMist® Quadrivalent) 2019-2020,medImmune,Cat # NDC 66017-306-01 Influenza

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Recombinant Human IFN-γ (carrier-free) from BioLegend

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Recombinant Human IFN-γ (carrier-free) from BioLegend

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