PerCP/Cyanine5.5 Streptavidin 405214 from BioLegend

Product Specs
ItemPerCP/Cyanine5.5 Streptavidin
CompanyBioLegend
Price $293.00Supplier Page View Company Product Page
Catalog Number405214
ApplicationsFC - Quality tested, ICFC - Verified
Size100 µg
Concentration0.2mg/ml (concentration relates to the Streptavidin only component of the conjugate)
FormatPhosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Conjugate/TagPerCP/Cyanine5.5
SpeciesHuman, Mouse, Rat, All Species
TargetBiotin
TypeStreptavidin

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BioLegend

8999 BioLegend Way

San Diego, California 92121

United States

Phone: 1-877-246-5343 Toll-Free, USA & Canada
1-858-768-5800 International
Fax: 1-877-455-9587

Company Profile

Website: biolegend.com

(1) Notch interaction with RUNX factors regulates initiation of the T-lineage programJ Exp MedFebruary 2, 2026Yuichi Kama, Ken-Ichi Hirano, Kaori Masuhara, Yusuke Endo, Yuka Suzuki, Masanori Fujimoto, Tatsuma Matsuda, Takashi Yahata, Masahiko Kato, Katsuto Hozumi, et al.RRID:AB_313427], and TCRγδ [118103; BioLegend, RRID:AB_313827]) was used, followed by streptavidin-PerCPCy5.5 (405214; BioLegend, RRID:AB_2716577). Prior to cell surface staining, cells were treated with an Fc receptor

(2) IgM and IgG1 B cell receptors differentially affect B cell fates and dictate the pathogenesis of mature B cell lymphomasSci AdvAugust 29, 2025Rebecca M. Southern, Rebecca L. Schmitz, Daniela Samaniego-Castruita, Daniel Balsells, Isabella Han, Prathyaya Ramesh, Mi Thao Tran, Carlos Montes, Taek-Chin Cheong, Roberto Chiarle, et al.APC/Cy7,BioLegend,110716 Anti-mouse CD19 FITC,BioLegend,115506 Streptavidin PerCP/Cy5.5,BioLegend,405214 Anti-mouse IgG1 FITC (A85-1),BD Biosciences,553443 Anti-mouse IgM PE/Cy7 (II/41),eBioscience,25-5790-82 Tomm20

(3) The immune response against cancer is modulated by stromal cell fibronectinNeoplasiaJune 30, 2025Alexander Lubosch, Lauren Pitt, Caren Zoeller, Franziska Wirth, Tarik Exner, Barbara Steigenberger, Guido Wabnitz, Jutta Schroeder-Braunstein, Inaam A. NakchbandiBiolegend,1:100,AB_571964 Ki67,PE,#652404 Biolegend,1:100,AB_2561525 Leptin receptorStreptavidin,BiotinPerCP-Cy5.5,#BAF497 R&D Systems#405214 Biolegend,1:501:400,AB_2296953AB_2716577 Ly6C,Alexa Fluor 647BV711PerCP-Cy5.5,#128010 Biolegend#128037

(4) Mitochondrial reactive oxygen species promote cancer metastasis and tumor microenvironment immunosuppression through gasdermin DCell death discoveryMay 6, 2025Miao N, Kang Z, Wang Z, Yu W, Liu T, Kong LZ, Zheng Y, Ding C, Zhang Z, Zhong C, et al.Biotin-CD45R/B220 (Cat#103204, Biolegend), Biotin-IL-7Rα (Cat#135006, Biolegend) and Percp-Cy5.5-Streptavidin (Cat#405214, Biolegend) antibodies) were used for lineage staining. PB-Sca-1 (Cat#108120, Biolegend), APC-CD117

(5) A genome-wide screen identifies genes required for erythroid differentiationNat CommunApril 12, 2025Greggory Myers, Ann Friedman, Lei Yu, Narges Pourmandi, Claire Kerpet, Masaki A. Ito, Rilie Saba, Vi Tang, Ayse Bilge Ozel, Ingrid L. Bergin, et al.103404), CD34 (eBioscience 11-0341-85), Thy1.2 (Biolegend 105304), CD71(Biolegend 113803) (SA Biolegend 405214). The lineage cocktail included antibodies against CD4, CD8, CD11c, GR1, Ter119, B220, CD19, Nk1.1,

(6) In-depth functional analysis of BRD9 in fetal hematopoiesis reveals context-dependent rolesiScienceMarch 21, 2025Yifan Zhang, Masaki Nomura, Koutarou Nishimura, Weijia Zang, Yui Koike, Muran Xiao, Hiromi Ito, Miki Fukumoto, Atsushi Tanaka, Yumi Aoyama, et al.PE/Cyanine7)",BioLegend,Cat#108114; RRID: AB_493596 Anti-Streptavidin (conjugated to PerCP/Cyanine5.5),BioLegend,Cat#405214 "Anti-CD11b (Clone M1/70, conjugated to PerCP/Cyanine5.5)",BioLegend,Cat#101228; RRID: AB_893232 "Anti-CD71

(7) Adult Withdrawal of Long-term Benzophenone-3 Treatment Induces Regression of Mammary Ductal Branching in a Diet-Dependent Manner**Food and Chemical ToxicologyMarch 1, 2025Calista Busch, Anastasia Kariagina, Elena Morozova, Mitchell A. Borin, Richard C. Schwartzanti-mouse CD119-biotin (0.4 μg; # 550482, BD Pharmingen) combined with Streptavidin-PerCP-Cy5.5 (0.1 μg; # 405214, Biolegend, San Diego, CA), and rat anti-mouse CD124-PE (0.16 μg; # 552509, BD Pharmingen). The staining

(8) An epitranscriptomic program maintains skeletal stem cell quiescence via a METTL3-FEM1B-GLI1 axisThe EMBO JournalFebruary 27, 2025Jing Wang, Weidong Liu, Tiantian Zhang, Manman Cui, Kexin Gao, Pengbo Lu, Shuxin Yao, Ziyan Cao, Yanbing Zheng, Wen Tian, et al.washed with PBS; then stained with fluorochrome-conjugated antibodies of PerCP/Cyanine5.5 Streptavidin (405214, BioLegend), CD51-PE (104106, BioLegend), Thy1.1-APC/Cyanine7 (202520, BioLegend), Thy1.2-APC/Cyanine7

(9) Influenza A Virus H7 nanobody recognizes a conserved immunodominant epitope on hemagglutinin head and confers heterosubtypic protectionNat CommunJanuary 9, 2025Zhao-Shan Chen, Hsiang-Chi Huang, Xiangkun Wang, Karin Schön, Yane Jia, Michael Lebens, Danica F. Besavilla, Janarthan R. Murti, Yanhong Ji, Aishe A. Sarshad, et al.Brilliant Violet 650 Streptavidin (BD, catalog: 563855), Percp5.5 Streptavidin (BioLegend, catalog: 405214) before the experiment: proteins were diluted at 0.02 mg/ml and labeled by stepwise addition (at 10

(10) Epi-microRNA mediated metabolic reprogramming counteracts hypoxia to preserve affinity maturationNature communicationsDecember 3, 2024Nakagawa R, Llorian M, Varsani-Brown S, Chakravarty P, Camarillo JM, Barry D, George R, Blackledge NP, Duddy G, Kelleher NL, et al.mouse IgM F(ab’)2 fragment, Jackson ImmunoResearch, 115-006-020; Streptavidin, PerCP-Cy5.5, BioLegend, 405214; Streptavidin, PE, ThermoFisher Sciences, 12-4317-87; Streptavidin, BV650, BioLegend, 405231; Streptavidin,

(11) HES1 is required for mouse fetal hematopoiesisStem cell research & therapyJuly 29, 2024Zhu AZ, Ma Z, Wolff EV, Lin Z, Gao ZJ, Li X, Du W.San Diego, CA; Cat: # 559971) followed by streptavidin PerCP-Cy5.5 (BioLegend, San Diego, CA; Cat: # 405214) antibody staining. In addition to the lineage markers for the analysis of LSK (Lin−Sca1+c-kit+) and

(12) BRD9 determines the cell fate of hematopoietic stem cells by regulating chromatin stateNature communicationsDecember 15, 2023Xiao M, Kondo S, Nomura M, Kato S, Nishimura K, Zang W, Zhang Y, Akashi T, Viny A, Shigehiro T, et al.BioLegend; 108114; 1:400); Sca1-FITC (D7; BioLegend; 108106; 1:200); Streptavidin-PerCPCy5.5 (BioLegend; 405214; 1:400); Streptavidin-BV605 (BioLegend; 405229; 1:400);CD45.1-FITC (A20; BD Pharmingen; 553775; 1:300);

(13) Wnt/β-Catenin-Signaling Modulates Megakaryopoiesis at the Megakaryocyte-Erythrocyte Progenitor Stage in the Hematopoietic SystemCellsDecember 4, 2023Yalcin BH, Macas J, Wiercinska E, Harter PN, Fawaz M, Schmachtel T, Ghiro I, Bieniek E, Kosanovic D, Thom S, et al.(AmsterdamThe Netherlands),122520,1:75, Streptavidin-PerCP/Cy5.5,,BioLegend (AmsterdamThe Netherlands),405214,1:300, Antibodies.

(14) Senescent immune cells accumulation promotes brown adipose tissue dysfunction during agingNature communicationsJune 2, 2023Feng X, Wang L, Zhou R, Zhou R, Chen L, Peng H, Huang Y, Guo Q, Luo X, Zhou H.Biotin anti-human CD3 Antibody (BioLegend, 317319, 1:20), Percp/Cyanine5.5 Streptavidin (BioLegend, 405214, 1:400), PE-anti-mouse-CD62L (BioLegend, 104407, 1:200), PE-anti-mouse-CXCR2 (BioLegend, 149303, 1:200),

(15) Polycomb repressive complex 1.1 coordinates homeostatic and emergency myelopoiesiseLifeJune 2, 2023Nakajima-Takagi Y, Oshima M, Takano J, Koide S, Itokawa N, Uemura S, Yamashita M, Andoh S, Aoyama K, Isshiki Y, et al.13-0411-85; RRID:AB_76348,FACS (0.1 μL/1 × 106 cell) Antibody,PerCP/Cyanine5.5 Streptavidin,BioLegend,Cat# 405214; RRID:AB_2716577,FACS (5 × 10–2 μL/1 × 106 cell) Antibody,APC anti-human/mouse CD45R (B220) (RA3-6B2)

(16) Dermal macrophages set pain sensitivity by modulating the amount of tissue NGF through an SNX25-Nrf2 pathwayNature immunologyMarch 1, 2023Tanaka T, Okuda H, Isonishi A, Terada Y, Kitabatake M, Shinjo T, Nishimura K, Takemura S, Furue H, Ito T, et al.(1:100, 116203, BioLegend), biotin anti-Ly6G (1:100, 127603, BioLegend), PerCP-Cy5.5–streptavidin (1:200, 405214, BioLegend) and APC–streptavidin (1:200, 405207, BioLegend). To exclude dead cells from analysis, cells

(17) Persistent DNA damage and oncogenic stress-induced Trem1 promotes leukemia in miceHaematologicaNovember 1, 2022Li X, Chatla S, Wilson AF, Wu L, Atale N, Du W.

(18) Transcription factor Zbtb1 interacts with bridging factor Lmo2 and maintains the T-lineage differentiation capacity of lymphoid progenitor cellsThe Journal of biological chemistryNovember 1, 2022Koizumi M, Kama Y, Hirano KI, Endo Y, Tanaka T, Hozumi K, Hosokawa H.CD19, TCRβ (BioLegend; 109204), and TCRγδ (BioLegend; 118103)) with streptavidin PerCPCy5.5 (BioLegend; 405214). Prior to cell surface staining, cells were treated with Fc blocker (Miltenyi). Two-step affinity purification

(19) The N(6)-methyladenosine methyltransferase METTL16 enables erythropoiesis through safeguarding genome integrityNature communicationsOctober 28, 2022Yoshinaga M, Han K, Morgens DW, Horii T, Kobayashi R, Tsuruyama T, Hia F, Yasukura S, Kajiya A, Cai T, et al.FITC Rat anti-mouse CD34 (Clone: RAM34, BD Pharmingen, #560238, 1:100), PerCP/Cy5.5 Streptavidin (Biolegend, #405214, 1:500), PE Rat anti-mouse Ter119 (Clone: TER-119, Biolegend, #116207, 1:200), PE Rat anti-mouse c-kit

(20) PD-1 expression on mouse intratumoral NK cells and its effects on NK cell phenotypeiScienceOctober 21, 2022Wagner AK, Kadri N, Tibbitt C, van de Ven K, Bagawath-Singh S, Oliinyk D, LeGresley E, Campbell N, Trittel S, Riese P, et al.124209RRID AB_1236464 Streptavidin PerCp Cy5.5,Biolegend,Cat# 405231 Streptavidin BV650,Biolegend,Cat# 405214 Ly49C (4LO3311),Gift,Susanne Lemieux Anti-CD16/CD32,Mabtech,N/A ,, "Chemicals, peptides, and recombinant

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7/24/2017Flexible Fluorophore for Stains Frank WuWe study T-cell mediated immunity in the lab and use a variety of antibodies to look at the hosts immune response to pathogens. Thus, flexibility in creating panels is extremely important, and this ... Read Review

PerCP/Cyanine5.5 Streptavidin from BioLegend

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PerCP/Cyanine5.5 Streptavidin from BioLegend

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