Thermostable Inorganic Pyrophosphatase M0296S from New England Biolabs

Product Specs
ItemThermostable Inorganic Pyrophosphatase
CompanyNew England Biolabs
Price $79.00 Inquire
Catalog NumberM0296S
Molecule NameThermostable Inorganic Pyrophosphatase
Concentration2,000 units/ml
Source/Expression SystemPurified from the extreme thermophile Thermococcus litoralis.
ApplicationsDNA Manipulation
Size250 units
TypeEnzymes
Temperature-20 C

Specifications/Features

Unit Definition:
One unit is the amount of enzyme that will generate 1 µmols of phosphate per minute from inorganic pyrophosphate under standard reaction conditions (a 10 minute reaction at 75°C in 50 mM Tricine [pH 8.5] , 1 mM MgCl₂, 0.32 mM PPi, reaction volume of 0.5 ml).

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New England Biolabs

240 County Road

Ipswich, Massachusetts 1938

United States

Phone: 800-632-5227
800-632-7799
978-921-1350

Company Profile

Website: http://www.neb.com

(1) The E3 ubiquitin ligase mechanism specifying targeted microRNA degradationNatureMarch 18, 2026Jakob Farnung, Elena Slobodyanyuk, Peter Y. Wang, Lianne W. Blodgett, Daniel H. Lin, Susanne von Gronau, Brenda A. Schulman, David P. BartelSUPERase•In (1 U µl−1; Invitrogen, AM2694) and Thermostable Inorganic Pyrophosphatase (0.0083 U µl−1; NEB, M0296). After incubation at 37 °C for 3–4 h, DNA templates were digested with RQ1 DNase (Promega, M6101) at

(2) Cas10 residues lining the target RNA binding channel regulate interference by distinguishing cognate target RNA from mismatched targetsRNA BiologyFebruary 18, 2026Sarah A. Khweis, Mason A. Blackburn, Calvin C. Perdigao, Megan O. Pierce, Colby R. Lewis, Jack A. Dunkleglycerol; 1 mM MgCl2; 50 to 1000 µM ATP; and 0.05 units Thermostable Inorganic Pyrophosphatase [TIPP, NEB M0296S]). Reactions were incubated at 37°C to initiate product formation. At various time points, aliquots

(3) Functional and structural characterization of Staphylococcus aureus N-acetylglucosamine 1-phosphate uridyltransferase (GlmU) reveals a redox-sensitive acetyltransferase activityProtein SciMarch 27, 2025Jordan L. Pederick, Akhil Kumar, Tara L. Pukala, John B. Bruningfrom Sigma (St. Louis, MO, USA). A 2 U/μL solution of thermostable inorganic pyrophosphatase (TIPP; M0296) was purchased from New England Biolabs. The reducing agents dithiothreitol (DTT; BIMB1015), tris(2‐carboxyethyl)phosphine

(4) The structural basis for RNA slicing by human Argonaute2Cell ReportsJanuary 28, 2025Abdallah A. Mohamed, Peter Y. Wang, David P. Bartel, Seychelle M. VosInvitrogen, AM2694), and thermostable Inorganic Pyrophosphatase (0.0083 U/μL, New England Biolabs, M0296). After incubation at 37°C for 3–4 h, then a further incubation with RQ1 DNase (Promega, M6101) at 37°C

(5) Optimal Chimeric Antigen Receptor (CAR)-mRNA for Transient CAR T Cell GenerationInternational journal of molecular sciencesJanuary 23, 2025Kitte R, Serfling R, Blache U, Seitz C, Schrader S, Köhl U, Fricke S, Bär C, Tretbar US.UTP/N1-methylpseudo-UTP, 5 mM GTP, 1 µg template DNA, 2 mU/µL Yeast Thermostable Inorganic Pyrophosphatase (#M0296S, New England Biolabs, Ipswich, MA, USA), 1 U/µL RNase Inhibitor (#N2515, Promega Corporation, Madison,

(6) Developing, Characterizing, and Modeling CRISPR-Based Point-of-Use Pathogen DiagnosticsACS Synthetic BiologyJanuary 17, 2025Jaeyoung K. Jung, Kathleen S. Dreyer, Kate E. Dray, Joseph J. Muldoon, Jithin George, Sasha Shirman, Maria D. Cabezas, Anne E. d’Aquino, Matthew S. Verosloff, Kosuke Seki, et al.NaCl, and 2 mM spermidine), 11.4 mM NTPs pH 7.5, 0.3 U thermostable inorganic pyrophosphatase (NEB #M0296S), 100 nM transcription template, 50 ng T7 RNAP, and Milli-Q ultrapure H2O to a total volume of 100 μL.

(7) Deconvolving organogenesis in space and time via spatial transcriptomics in thick tissuesbioRxivSeptember 27, 2024Soichiro Asami, Chenshuo Yin, Luis A. Garza, Reza KalhorPhi29 DNA Polymerase Reaction Buffer (New England Biolabs, #M0269L), Thermostable Inorganic Pyrophosphatase (New England Biolabs, ,#M0296L), RNase Inhibitor Murine (New England Biolabs, #RNase Inhibitor Murine), Acryloyl-X (Invitrogen, #A20770),

(8) The guide-RNA sequence dictates the slicing kinetics and conformational dynamics of the Argonaute silencing complexMolecular CellAugust 8, 2024Peter Y. Wang, David P. BartelU/μL, Invitrogen, AM2694), thermostable Inorganic Pyrophosphatase (0.0083 U/μL, New England Biolabs, M0296), and T7 RNA Polymerase (purified in-house). The reaction was incubated at 37°C for 3–4 h, before RQ1

(9) RNA sequences that direct selective ADAR editing from a SELEX library bearing 8-azanebularineBioorganic & Medicinal ChemistryApril 15, 2024Bailey L. Wong, Herra G. Mendoza, Casey S. Jacobsen, Peter A. Bealpolymerase buffer, 3.5 U/μL T7 RNA polymerase (Thermo EP0113), 25 U/mL inorganic pyrophosphatase (NEB M0296S), 0.2 mM each of ATP, UTP, CTP, GTP, and incubated at 37 °C overnight. After overnight incubation, 2

(10) Development of a loop-mediated isothermal amplification (LAMP)-based electrochemical test for rapid detection of SARS-CoV-2iScienceSeptember 15, 2023Khushboo Borah Slater, Muhammad Ahmad, Aurore Poirier, Ash Stott, Bianca Sica Siedler, Matthew Brownsword, Jai Mehat, Joanna Urbaniec, Nicolas Locker, Yunlong Zhao, et al.(NEB),",New England Biolabs,M0380S 2000U/mL thermostable inorganic pyrophosphatase,New England Biolabs,M0296S SYBR green nucleic acid stain,Fisher scientific UK,10710004 nuclease free water,New England Biolabs,B1500S sodium

(11) Dynamically regulated two-site interaction of viral RNA to capture host translation initiation factorNature CommunicationsAugust 28, 2023Shunsuke Imai, Hiroshi Suzuki, Yoshinori Fujiyoshi, Ichio Shimadamuscle (MK, Sigma-Aldrich M3003), and thermostable inorganic pyrophosphatase (TIPP, New England Biolabs M0296L). The [{1′, 2′, 3′, 4′, 5′, 5′′}-2H, 13C8] GTP was enzymatically synthesized by using D-[1, 2, 3, 4,

(12) Repurposing of artesunate, an antimalarial drug, as a potential inhibitor of hepatitis E virusArchives of virologyApril 28, 2023Bhise N, Agarwal M, Thakur N, Akshay PS, Cherian S, Lole K.to remove any contaminating RNase, and 5 U of thermostable inorganic pyrophosphatase (NEB, cat. no. M0296) and 0.5 µM of in vitro-synthesized 3’NCR HEV RNA template were added as described previously [59].

(13) Binding of 2′,3′-Cyclic Nucleotide Monophosphates to Bacterial Ribosomes Inhibits TranslationACS Central ScienceNovember 23, 2022Shikha S. Chauhan, Nick J. Marotta, Anna C. Karls, Emily E. Weinertkit (cat. #E6800), DNase I enzyme (cat. #M0303), Thermostable Inorganic Pyrophosphatase (TIPP; cat. #M0296), T7 RNA polymerase (cat. #M0251), RNase inhibitor (cat. #M0314), BSA (cat. #B9000), and nuclease-free

(14) Engineered helicase replaces thermocycler in DNA amplification while retaining desired PCR characteristicsNature CommunicationsOctober 23, 2022MomÄilo Gavrilov, Joshua Y. C. Yang, Roger S. Zou, Wen Ma, Chun-Ying Lee, Sonisilpa Mohapatra, Jimin Kang, Ting-Wei Liao, Sua Myong, Taekjip Hamg/mL), PcrA M6 or M5 helicase (0.2 mg/mL), Bst-LF DNAP (1.5 mg/mL), PPase (2000 units/mL, NEB catalog # M0296S). Other stock components are dNTP’s (10 mM each), ATP (100 mM), Evagreen dye (20X from Biotium # 31000),

(15) Multiplex Target-Redundant RT-LAMP for Robust Detection of SARS-CoV-2 Using Fluorescent Universal Displacement ProbesMicrobiology SpectrumAugust 31, 2022Enos C. Kline, Nuttada Panpradist, Ian T. Hull, Qin Wang, Amy K. Oreskovic, Peter D. Han, Lea M. Starita, Barry R. Lutz(NEB, M0380L), 0.7 μg TFpol polymerase, and 2 units of thermostable inorganic pyrophosphatase (NEB, M0296L). 5 μL of extracted RNA was added to 15 μL mLAMP reaction mixture and incubated at 63.3°C for 1 h on

(16) Long-term hepatitis B virus infection of rhesus macaques requires suppression of host immunityNature communicationsMay 30, 2022Biswas S, Rust LN, Wettengel JM, Yusova S, Fischer M, Carson JN, Johnson J, Wei L, Thode T, Kaadige MR, et al.New England Biolabs, Catalog# N0447S), 0.1 μL pyrophosphatase (2 U/μL, New England Biolabs, Catalog# M0296S), and 1 μL phi29 DNA Polymerase (10 U/μL, New England Biolabs, Catalog# M0296L) in 1.3 μL water and

(17) Programming cell-free biosensors with DNA strand displacement circuitsNature Chemical BiologyApril 1, 2022Jaeyoung K. Jung, Chloé M. Archuleta, Khalid K. Alam, Julius B. LucksNTPs pH 7.5, 0.3 units (U) of thermostable inorganic pyrophosphatase (New England Biolabs, catalog no. M0296S), 100 nM transcription template, 50 ng of T7 RNAP and MilliQ ultrapure H2O to a total volume of 500

(18) Synthetic logic circuits using RNA aptamer against T7 RNA polymeraseBiotechnology JournalMarch 1, 2022Jongmin Kim, Juan F. Quijano, Jeongwon Kim, Enoch Yeung, Richard M. Murraybuffer and thermostable inorganic pyrophosphatase (New England Biolabs, Ipswich, MA, USA; #B9012S, #M0296S), NTP (Epicentre, Madison, WI, USA; #RN02825) were purchased. Malachite green dye was purchased from

(19) Harmony COVID-19: A ready-to-use kit, low-cost detector, and smartphone app for point-of-care SARS-CoV-2 RNA detectionScience AdvancesDecember 17, 2021Nuttada Panpradist et al.each deoxynucleotide triphosphate (dNTP), RNase inhibitor (1 U/μl; N2615, Promega), TiPP (25 mU/μl; M0296L, New England Biolabs), IAC DNA (250 copies/μl), 6 mM magnesium sulfate, and 1× ThermoPol buffer (B9004S,

(20) SCOPE enables type III CRISPR-Cas diagnostics using flexible targeting and stringent CARF ribonuclease activationNature CommunicationsDecember 1, 2021Jurre A. Steens et al.followed by an incubation at 25 °C for 30 min (Fig. 2). Alternatively, thermostable pyrophosphatase (NEB #M0296) was added during the 1 h incubation at 65 °C (Fig. 3). cOA quantification was achieved by using the

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Thermostable Inorganic Pyrophosphatase

Thermostable Inorganic Pyrophosphatase from New England Biolabs

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Specifications/Features

New England Biolabs

Citations (30)

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Thermostable Inorganic Pyrophosphatase from New England Biolabs

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