Thermostable Inorganic Pyrophosphatase M0296S from New England Biolabs

Product Specs
ItemThermostable Inorganic Pyrophosphatase
CompanyNew England Biolabs
Price Pricing Info Supplier Page View Company Product Page
Catalog NumberM0296S
Molecule NameThermostable Inorganic Pyrophosphatase
Concentration2,000 units/ml
Source/Expression SystemPurified from the extreme thermophile Thermococcus litoralis.
ApplicationsDNA Manipulation
Quantity250 units
Brand/TrademarkNew England Biolabs Inc (NEB)
TypeEnzymes
Temperature-20 C

Specifications/Features

Unit Definition:
One unit is the amount of enzyme that will generate 1 µmols of phosphate per minute from inorganic pyrophosphate under standard reaction conditions (a 10 minute reaction at 75°C in 50 mM Tricine [pH 8.5] , 1 mM MgCl₂, 0.32 mM PPi, reaction volume of 0.5 ml).

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New England Biolabs

240 County Road

Ipswich, Massachusetts 1938

United States

Phone: 800-632-5227
800-632-7799
978-921-1350

Company Profile

Website: http://www.neb.com

(1) Development of a loop-mediated isothermal amplification (LAMP)-based electrochemical test for rapid detection of SARS-CoV-2iScienceSeptember 15, 2023Khushboo Borah Slater, Muhammad Ahmad, Aurore Poirier, Ash Stott, Bianca Sica Siedler, Matthew Brownsword, Jai Mehat, Joanna Urbaniec, Nicolas Locker, Yunlong Zhao, et al.(NEB),",New England Biolabs,M0380S 2000U/mL thermostable inorganic pyrophosphatase,New England Biolabs,M0296S SYBR green nucleic acid stain,Fisher scientific UK,10710004 nuclease free water,New England Biolabs,B1500S sodium

(2) Dynamically regulated two-site interaction of viral RNA to capture host translation initiation factorNature CommunicationsAugust 28, 2023Shunsuke Imai, Hiroshi Suzuki, Yoshinori Fujiyoshi, Ichio Shimadamuscle (MK, Sigma-Aldrich M3003), and thermostable inorganic pyrophosphatase (TIPP, New England Biolabs M0296L). The [{1′, 2′, 3′, 4′, 5′, 5′′}-2H, 13C8] GTP was enzymatically synthesized by using D-[1, 2, 3, 4,

(3) Repurposing of artesunate, an antimalarial drug, as a potential inhibitor of hepatitis E virusArchives of VirologyMay 1, 2023Neha Bhise, Megha Agarwal, Nidhi Thakur, P. S. Akshay, Sarah Cherian, Kavita Loleto remove any contaminating RNase, and 5 U of thermostable inorganic pyrophosphatase (NEB, cat. no. M0296) and 0.5 µM of in vitro-synthesized 3’NCR HEV RNA template were added as described previously [59].

(4) Long-term hepatitis B virus infection of rhesus macaques requires suppression of host immunityNature CommunicationsDecember 1, 2022Sreya Biswas et al.New England Biolabs, Catalog# N0447S), 0.1 μL pyrophosphatase (2 U/μL, New England Biolabs, Catalog# M0296S), and 1 μL phi29 DNA Polymerase (10 U/μL, New England Biolabs, Catalog# M0296L) in 1.3 μL water and

(5) Binding of 2′,3′-Cyclic Nucleotide Monophosphates to Bacterial Ribosomes Inhibits TranslationACS Central ScienceNovember 23, 2022Shikha S. Chauhan, Nick J. Marotta, Anna C. Karls, Emily E. Weinertkit (cat. #E6800), DNase I enzyme (cat. #M0303), Thermostable Inorganic Pyrophosphatase (TIPP; cat. #M0296), T7 RNA polymerase (cat. #M0251), RNase inhibitor (cat. #M0314), BSA (cat. #B9000), and nuclease-free

(6) Engineered helicase replaces thermocycler in DNA amplification while retaining desired PCR characteristicsNature CommunicationsOctober 23, 2022MomÄilo Gavrilov, Joshua Y. C. Yang, Roger S. Zou, Wen Ma, Chun-Ying Lee, Sonisilpa Mohapatra, Jimin Kang, Ting-Wei Liao, Sua Myong, Taekjip Hamg/mL), PcrA M6 or M5 helicase (0.2 mg/mL), Bst-LF DNAP (1.5 mg/mL), PPase (2000 units/mL, NEB catalog # M0296S). Other stock components are dNTP’s (10 mM each), ATP (100 mM), Evagreen dye (20X from Biotium # 31000),

(7) Multiplex Target-Redundant RT-LAMP for Robust Detection of SARS-CoV-2 Using Fluorescent Universal Displacement ProbesMicrobiology SpectrumAugust 31, 2022Enos C. Kline, Nuttada Panpradist, Ian T. Hull, Qin Wang, Amy K. Oreskovic, Peter D. Han, Lea M. Starita, Barry R. Lutz(NEB, M0380L), 0.7 μg TFpol polymerase, and 2 units of thermostable inorganic pyrophosphatase (NEB, M0296L). 5 μL of extracted RNA was added to 15 μL mLAMP reaction mixture and incubated at 63.3°C for 1 h on

(8) Programming cell-free biosensors with DNA strand displacement circuitsNature Chemical BiologyApril 1, 2022Jaeyoung K. Jung, Chloé M. Archuleta, Khalid K. Alam, Julius B. LucksNTPs pH 7.5, 0.3 units (U) of thermostable inorganic pyrophosphatase (New England Biolabs, catalog no. M0296S), 100 nM transcription template, 50 ng of T7 RNAP and MilliQ ultrapure H2O to a total volume of 500

(9) Synthetic logic circuits using RNA aptamer against T7 RNA polymeraseBiotechnology JournalMarch 1, 2022Jongmin Kim, Juan F. Quijano, Jeongwon Kim, Enoch Yeung, Richard M. Murraybuffer and thermostable inorganic pyrophosphatase (New England Biolabs, Ipswich, MA, USA; #B9012S, #M0296S), NTP (Epicentre, Madison, WI, USA; #RN02825) were purchased. Malachite green dye was purchased from

(10) Harmony COVID-19: A ready-to-use kit, low-cost detector, and smartphone app for point-of-care SARS-CoV-2 RNA detectionScience AdvancesDecember 17, 2021Nuttada Panpradist et al.each deoxynucleotide triphosphate (dNTP), RNase inhibitor (1 U/μl; N2615, Promega), TiPP (25 mU/μl; M0296L, New England Biolabs), IAC DNA (250 copies/μl), 6 mM magnesium sulfate, and 1× ThermoPol buffer (B9004S,

(11) Point-of-care bulk testing for SARS-CoV-2 by combining hybridization capture with improved colorimetric LAMPNature CommunicationsDecember 1, 2021Lukas Bokelmann, Olaf Nickel, Tomislav Maricic, Svante Pääbo, Matthias Meyer, Stephan Borte, Stephan Riesenberg0.1 ng/µl Tte UvrD Helicase (NEB, #M1202S), 0.05 U/µl thermostable inorganic pyrophosphatase (NEB, #M0296L), 0.4 U/µl Protector RNase inhibitor (Sigma Aldrich, St. Louis, MI, USA, # 03335402001) (Supplementary

(12) SCOPE enables type III CRISPR-Cas diagnostics using flexible targeting and stringent CARF ribonuclease activationNature CommunicationsDecember 1, 2021Jurre A. Steens et al.followed by an incubation at 25 °C for 30 min (Fig. 2). Alternatively, thermostable pyrophosphatase (NEB #M0296) was added during the 1 h incubation at 65 °C (Fig. 3). cOA quantification was achieved by using the

(13) Single-copy detection of somatic variants from solid and liquid biopsyScientific ReportsDecember 1, 2021Ana-Luisa Silva, Paulina Klaudyna Powalowska, Magdalena Stolarek, Eleanor Ruth Gray, Rebecca Natalie Palmer, Bram Herman, Cameron Alexander Frayling, Barnaby William Balmforth1.13 µL Syto82 dye (S11363, Thermo Fisher Scientific), 0.08 µL Thermostable inorganic pyrophosphatase (M0296, New England Biolabs), 0.16 µL of primer mix (20 µM stock, 5′-T*C*GCAACATCCTATATCTGC-3′ and 5′-T*G*AGCTTTGACAATACTTGA-3′)

(14) CRISPR-Associated Primase-Polymerases are implicated in prokaryotic CRISPR-Cas adaptationNature CommunicationsDecember 1, 2021Katerina Zabrady, Matej Zabrady, Peter Kolesar, Arthur W. H. Li, Aidan J. Dohertypyrophosphate (PPi) upon phosphodiester bond formation and a thermostable inorganic pyrophosphatase, TiPP (M0296L, NEB) converts PPi to inorganic phosphate (Pi), which is then detected by the Malachite Green Phosphate

(15) Cell-free biosensors for rapid detection of water contaminantsNature biotechnologyDecember 1, 2020Jung JK, Alam KK, Verosloff MS, Capdevila DA, Desmau M, Clauer PR, Lee JW, Nguyen PQ, Pastén PA, Matiasek SJ, et al.2.25 mM DFHBI-1T, 11.4 mM Tris-buffered NTPs pH 7.5, 0.3U thermostable inorganic pyrophosphatase (#M0296S, New England Biolabs), DNA transcription template(s), and MilliQ H2O to a total volume of 20 µL. Regulated

(16) Nonredox thiolation in tRNA occurring via sulfur activation by a [4Fe-4S] clusterProceedings of the National Academy of SciencesJuly 11, 2017Simon Arragain, Ornella Bimai, Pierre Legrand, Sylvain Caillat, Jean-Luc Ravanat, Nadia Touati, Laurent Binet, Mohamed Atta, Marc Fontecave, Béatrice Golinelli-PimpaneauThermostable inorganic pyrophosphatase from Thermococcus litoralis was bought from New England Biolabs (M0296L). For the labeling experiment, 35S-sulfide was first formed by incubating 500 μM L-cysteine in the presence

(17) Reversibly constraining spliceosome-substrate complexes by engineering disulfide crosslinksMethodsJune 20, 2017Patrick McCarthy, Erin Garside, Yonatan Meschede-Krasa, Andrew MacMillan, Daniel Pomeranz Krummel

(18) Label-free selection of RNA aptamers for metabolic engineeringMethodsAugust 1, 2016Chuhern Hwang, James M. CarothersmM each of the other rNTPs were added along with 1 U/mL Thermostable Inorganic Pyrophosphatase (NEB M0296), 2.5 U/μL T7 RNA Polymerase (NEB M0251), and 1 U/μL RiboLock RNase inhibitor (ThermoFisher EO0381). Transcription

(19) Synthetic circuit for exact adaptation and fold-change detectionNucleic Acids ResearchMay 14, 2014Jongmin Kim, Ishan Khetarpal, Shaunak Sen, Richard M. Murraybuffer and thermostable inorganic pyrophosphatase (New England Biolabs, Ipswich, MA, USA; #B9012S, #M0296S), NTP and RNase R (Epicentre, Madison, WI, USA; #RN02825, #RNR07250) were purchased. Malachite Green

(20) Genome-Wide Analyses Reveal a Role for Peptide Hormones in Planarian Germline DevelopmentPLoS BiologyOctober 12, 2010James J. Collins, Xiaowen Hou, Elena V. Romanova, Bramwell G. Lambrus, Claire M. Miller, Amir Saberi, Jonathan V. Sweedler, Phillip A. Newmarkspermidine, 0.1 M DTT), 1 µL thermostable inorganic pyrophosphatase (New England Biolabs, Madison, WI, M0296S), 0.5 µL Optizyme recombinant ribonuclease inhibitor (Fisher Scientific, Pittsburg, PA, BP3222-5), and

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Thermostable Inorganic Pyrophosphatase

Thermostable Inorganic Pyrophosphatase from New England Biolabs

Description

Specifications/Features

New England Biolabs

Citations (21)

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Thermostable Inorganic Pyrophosphatase from New England Biolabs

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