Description
- Isolated from a recombinant source
- Supplied with 10X Reaction Buffer
Description:
Human apurinic/apyrimidinic (AP) endonuclease, APE 1, also known as HAP 1 or Ref-1, shares homology with
Escherichia coli exonuclease III protein. APE 1 cleaves the phosphodiester backbone immediately 5´ to an AP site, via hydrolytic mechanism, to generate a single-strand DNA break leaving a 3´-hydroxyl and 5´ -deoxyribose phosphate terminus. Besides AP endonuclease activity, APE 1 has also been reported to have weak DNA 3´ -diesterase, 3´ to 5´ exonuclease and RNase H activities .
In addition to DNA repair activity, APE 1 is also capable of regulating the DNA binding activity of many transcription factors
in vitro by a redox mechanism (Ref-1). As part of this process, APE 1 has been shown to stimulate the DNA binding activity of Fos-Jun heterodimers, Jun-Jun homodimers and Hela cell AP-1 proteins as well as that of several other transcription factors including NF-kB, Myb and members of the ATF/CREB family .
An
E. coli strain which carries the cloned human APE 1 gene
Applications:
- Single cell gel electrophoresis (Comet assay)
- Alkaline elution
- Alkaline unwinding
- Modified nick translation
Reagents Supplied:
NEBuffer 4 (10X)