Histone H4 Human, Recombinant M2504S from New England Biolabs

Product Specs
ItemHistone H4 Human, Recombinant
CompanyNew England Biolabs
Price $95.00 Inquire
Catalog NumberM2504S
Molecule NameHistone H4
Source/Expression SystemRecombinant
ApplicationsFunctional Genomics
TypeProteins
Temperature-20 C
Size100 µg

Add to Compare List

New England Biolabs

240 County Road

Ipswich, Massachusetts 1938

United States

Phone: 800-632-5227
800-632-7799
978-921-1350

Company Profile

Website: http://www.neb.com

(1) Nano-scaled, fully automated hydrogen/deuterium exchange for analysis of macromolecular assemblies reaching the MDa scaleMolecular & Cellular ProteomicsMarch 1, 2026Shaunak Raval, Yuqi Shi, Julia W. Morriss, Alexander R. Ivanov, Stephen J. Coales, Inna Aphasizheva, Ruslan Aphasizhev, Brian B. Liau, Steven A. Carr, Malvina Papanastasioufrom Fisher and HPLC water from J.T.Baker (JT4218–3). Human recombinant histones (H3.1; M2503S and H4; M2504S) were purchased from New England BioLabs Inc. ΝanoVipers of various lengths were purchased from Thermo

(2) Extracellular Histones as Exosome Membrane Proteins Regulated by Cell StressJournal of extracellular vesiclesFebruary 1, 2025Singh B, Fredriksson Sundbom M, Muthukrishnan U, Natarajan B, Stransky S, Görgens A, Nordin JZ, Wiklander OPB, Sandblad L, Sidoli S, et al.recombinant histones from New England Biolabs: H1 (M2501S), H2A (M2502S), H2B (M2505S), H3.1 (M2503S) and H4 (M2504S), total histones extracted from calf thymus (H9250, Type IIA Sigma‐Aldrich), or recombinant His‐tagged

(3) Sensitive Detection of Histones and γ-H2AX by Immunoblotting: Problems and SolutionsChemical research in toxicologySeptember 16, 2024Krawic C, Luczak MW, Zhitkovich A.Procedures Materials Individual histones were purchased from New England Biolabs (H2A: M2502S, H2B: M2505S, H3.1: M2507S, H4: M2504S), Epicypher (H2AX: 15–0307) and Sigma (H2AZ: 14–1109). Bleomycin was obtained from LKT Laboratories

(4) Protocol for an in vitro assay to study HIV-1 Tat methylationSTAR ProtocolsDecember 1, 2023Daniela Boehm, Katrin Kaehlcke, Martina Schnoelzer, Melanie Ott,New England Biolabs,Cat# M2507S; Lot# 0021508 "Histone H4, human, recombinant",New England Biolabs,Cat# M2504S; Lot# 0061508 "HIV-1 Tat aa1-72, recombinant",German Cancer Research CenterPagans et al.2,N/A "NF-κB

(5) A crucial role for dynamic expression of components encoding the negative arm of the circadian clockNature CommunicationsJune 8, 2023Bin Wang, Xiaoying Zhou, Arminja N. Kettenbach, Hugh D. Mitchell, Lye Meng Markillie, Jennifer J. Loros, Jay C. DunlapRecombinant human histone H2A (New England BioLabs, Catalog # M2502S) or H4 (New England BioLabs, Catalog # M2504S) respectively was added to the mixture at a final concentration of 0.1 mg/mL. Acetylation reactions

(6) TRAF6 Promotes PRMT5 Activity in a Ubiquitination-Dependent MannerCancersApril 27, 2023Liu L, Yin S, Gan W.methylation assays were performed as previously described [18]. Briefly, 1 μg of recombinant Histone H4 (M2504S, NEB, Ipswich, MA, USA) was incubated with PRMT5 immunoprecipitated from cells in the methylation buffer

(7) CDK5-PRMT1-WDR24 signaling cascade promotes mTORC1 signaling and tumor growthCell reportsApril 25, 2023Yin S, Liu L, Ball LE, Wang Y, Bedford MT, Duncan SA, Wang H, Gan W.Media,Vector Laboratories,Cat# H-1400–10 Histone H4 recombination protein,New England BioLabs,Cat# M2504S GSK3368715,MedChemExpress,Cat# HY-128717A SHURMount media,General Data,Cat# 682188 "Adenosyl-L-Methionine,

(8) Histone 4 lysine 5/12 acetylation enables developmental plasticity of Pristionchus mouth formNature communicationsApril 13, 2023Werner MS, Loschko T, King T, Reich S, Theska T, Franz-Wachtel M, Macek B, Sommer RJ.purification were determined by using a calibration curve of recombinant H3 and H4 (NEB, cat. #M2503S, #M2504S, respectively). Absolute amounts of histone were determined by densitometry on coomassie-stained bands

(9) The lysine methyltransferase SMYD5 amplifies HIV-1 transcription and is post-transcriptionally upregulated by Tat and USP11Cell reportsMarch 28, 2023Boehm D, Lam V, Schnolzer M, Ott M.,New England Biolabs,Cat# M2507S; Lot# 0021508 "Histone H4, Human, Recombinant",New England Biolabs,Cat# M2504S; Lot# 0061508 "HIV-1 Tat aa1-72, Recombinant",German Cancer Research Center Pagans et al.61,N/A Micrococcal Nuclease

(10) Cytotoxic CD8(+) T cells target citrullinated antigens in rheumatoid arthritisNature communicationsJanuary 19, 2023Moon JS, Younis S, Ramadoss NS, Iyer R, Sheth K, Sharpe O, Rao NL, Becart S, Carman JA, James EA, et al.H2B, H3.1 and H4 (Cayman Chemical; 25151, Thermo Fisher; RP43142, New England Biolabs; M2505S, M2503S, M2504S). To block the interaction of HLA class complex and CD8+ T cells, purified anti-human CD8 and/or HLA-A, B, C antibody

(11) Poly ADP-ribosylation of SET8 leads to aberrant H4K20 methylation in mammalian nuclear genomeCommunications biologyNovember 25, 2022Estève PO, Sen S, Vishnu US, Ruse C, Chin HG, Pradhan S.AdoMet (Perkin Elmer Life Science # NET155V001MC). Recombinant human histone H4 (New England Biolabs # M2504S) was used as a substrate. Filter disc method was used to process the samples and the [3H]CH3 incorporated

(12) Induction of broadly reactive influenza antibodies increases susceptibility to autoimmunityCell reportsMarch 8, 2022Labombarde JG, Pillai MR, Wehenkel M, Lin CY, Keating R, Brown SA, Crawford JC, Brice DC, Castellaw AH, Mandarano AH, et al.,New England BioLabs,Cat# M2505S H3 (histone),New England BioLabs,Cat# M2506S H4 (histone),New England BioLabs,Cat# M2504S Insulin (human recombinant),Fitzgerald,Cat# 30-AI51 Insulin (mouse recombinant),R&D Systems,Cat# 7544-MR LPS

(13) The macromolecular complexes of histones affect protein arginine methyltransferase activitiesJournal of Biological ChemistryOctober 1, 2021Melody D. Fulton, Mengtong Cao, Meng-Chiao Ho, Xinyang Zhao, Y. George ZhengH2A (catalog no. M2502S), H2B (catalog no. M2505S), H3.3 (catalog no. M2507S), and H4 (catalog no. M2504S) were purchased from New England Biolabs. The following items were purchased from EpiCypher, including

(14) Local states of chromatin compaction at transcription start sites control transcription levelsNucleic Acids ResearchAugust 20, 2021Satoru Ishihara, Yohei Sasagawa, Takeru Kameda, Hayato Yamashita, Mana Umeda, Naoe Kotomura, Masayuki Abe, Yohei Shimono, Itoshi Nikaidoelectrophoresis. As a control, recombinant histones H1.0, H3.1, H2B, and H4 (#M2501S, #M2503S, #M2505S and #M2504S, respectively, New England Biolabs) were utilized. Recombinant H1.0 and H3.1, or H2B and H4, were mixed

(15) DNA length tunes the fluidity of DNA-based condensatesBiophysical journalApril 6, 2021Muzzopappa F, Hertzog M, Erdel F.Briefly, 0.5 μg of DNA was mixed with equimolar quantities of histones H2A/H2B (NEB, M2508S), H3.1 (NEB, M2504S), and H4 (NEB, M2508S) relative to the number of nucleosome binding sites (defined as 200 bp, including

(16) Identification of lysine isobutyrylation as a new histone modification markNucleic acids researchJanuary 11, 2021Zhu Z, Han Z, Halabelian L, Yang X, Ding J, Zhang N, Ngo L, Song J, Zeng H, He M, et al.in vitro histone H4 isobutyrylation 1 μg of human recombinant histone H4 (New England BioLabs, Cat# M2504S) was incubated with 50 μM n-butyryl- or isobutyryl-CoA and 0.2 μM of HAT1 at 30°C for 1 h. The reaction

(17) Dual protease type XIII/pepsin digestion offers superior resolution and overlap for the analysis of histone tails by HX-MSMethodsDecember 1, 2020James Mullahoo, Terry Zhang, Karl Clauser, Steven A. Carr, Jacob D. Jaffe, Malvina Papanastasiou(MS2). Experimental Section Materials Human recombinant histones (H2A; M2502S, H2B.1; M2505S, H3.1; M2503S and H4; M2504S) were purchased from New England BioLabs Inc (Ipswich, MA). Mononucleosomes, recombinant human (16–0009)

(18) The microtubule-associated histone methyltransferase SET8, facilitated by transcription factor LSF, methylates α-tubulinThe Journal of biological chemistryApril 3, 2020Chin HG, Esteve PO, Ruse C, Lee J, Schaus SE, Pradhan S, Hansen U.(pH 8.0) and 0.5 mm DTT at room temperature overnight. As indicated, histone H4 (New England Biolabs, M2504S), recombinant His-LSF protein, or FQI1 inhibitor was added to the reaction. Samples were separated by

(19) Non-enzymatic Lysine Lactoylation of Glycolytic EnzymesCell Chemical BiologyFebruary 1, 2020Dominique O. Gaffney et al.2013",N/A Methylglyoxal,MP Biomedicals,Cat# 02155558-CF; CAS: 78-98-8 Histone H4,New England Biolabs,Cat# M2504S "GSH-(glycine-l3C2,l5N, internal standard)",MilliporeSigma,Cat# 683620 (1-Carboxyethyl)-L-lysine-d4,Toronto

(20) Elevated H3K79 homocysteinylation causes abnormal gene expression during neural development and subsequent neural tube defectsNature CommunicationsDecember 1, 2018Qin Zhang et al.in vitro Purified histones, including H2a (M2502S, NEB), H2b (M2505S, NEB), H3 (M2503S, NEB) and H4 (M2504S, NEB) were selected. Reactions were carried out at 37 °C in mixtures containing 0.5 mM L-HTL hydrochloride,

Be the First to Write a Review!

Histone H4 Human, Recombinant

Histone H4 Human, Recombinant from New England Biolabs

Description

New England Biolabs

Citations (34)

Reviews

Histone H4 Human, Recombinant from New England Biolabs

About Biocompare

Advertise with Us

Specialized Search Tools

Site Map