Histone H2B Human, Recombinant M2505S from New England Biolabs

Product Specs
ItemHistone H2B Human, Recombinant
CompanyNew England Biolabs
Price $95.00 Inquire
Catalog NumberM2505S
Source/Expression SystemRecombinant
Molecule NameHistone H2B
ApplicationsFunctional Genomics
TypeProteins
Temperature-20 C
Size100 µg

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New England Biolabs

240 County Road

Ipswich, Massachusetts 1938

United States

Phone: 800-632-5227
800-632-7799
978-921-1350

Company Profile

Website: http://www.neb.com

(1) MPND Loss Epigenetically Activates TGFβ/SMAD3 Signaling to Drive Tumor Progression and Metastasis in Non–Small Cell Lung CancerCancer ResMay 26, 2026Jianfeng Yu, Jieru Zhang, Jun Zhang, Zhenqi Bai, Yunqing Zhang, Zi’an Liu, Lifan Su, Ye Yang, Yixin Lian, Wei Gu, et al.Motif), 10 μg of recombinant histone H2A (M2502S, New England Biolabs), 10 μg of recombinant histone H2B (M2505S, New England Biolabs), or 10 μg of recombinant histone H4 (M2504S, New England Biolabs) in 500 μL of

(2) Simultaneous inhibition of TRIM24 and TRIM28 sensitises prostate cancer cells to antiandrogen therapy, decreasing VEGF signalling and angiogenesisMolecular OncologyMay 24, 2025Damien A. Leach, Nilesh Chatterjee, Kellie Spahr, Gilberto Serrano de Almeida, Anabel Varela‐Carver, Taimur T. Shah, Mathias Winkler, Hashim U. Ahmed, Charlotte L. Bevanto enhance protein pulldown, human control RNA (4307281, Thermo Fisher) and recombinant histone 2B (M2505S, New England Biolabs) were added to the immunoprecipitation steps [48, 49]. 2.9 Immunofluorescence (IF

(3) Protocol for an in vitro assay to study HIV-1 Tat methylationSTAR ProtocolsDecember 1, 2023Daniela Boehm, Katrin Kaehlcke, Martina Schnoelzer, Melanie Ott,New England Biolabs,Cat# M2502S; Lot# 0131508 "Histone H2B, human, recombinant",New England Biolabs,Cat# M2505S; Lot# 0031508 "Histone H3.1, human, recombinant",New England Biolabs,Cat# M2503S; Lot# 0041504 "Histone H3.2, human, recombinant

(4) Targeting the COMMD4–H2B protein complex in lung cancerBritish Journal of CancerNovember 1, 2023Ming Tang, Joshua T. Burgess, Mark Fisher, Didier Boucher, Emma Bolderson, Neha S. Gandhi, Kenneth J. O’Byrne, Derek J. Richard, Amila Suraweeraof recombinant GST-COMMD4 (Abnova, H00054939-P01) was incubated with 1 μg of recombinant H2B (NEB, M2505S) in NP40 buffer for 30 min at 4 °C. For competition experiments, 1 μg (or the amount specified) of the

(5) Identification of a Protein Arginine Methyltransferase 7 (PRMT7)/Protein Arginine Methyltransferase 9 (PRMT9) InhibitorJournal of medicinal chemistryOctober 12, 2023Feoli A, Iannelli G, Cipriano A, Milite C, Shen L, Wang Z, Hadjikyriacou A, Lowe TL, Safaeipour C, Viviano M, et al.described [human GST-SF3B2 (401–550);13C. elegans SFTB-2;48 human histone H2B (New England Biolabs, M2505S)]. Xenopus laevis histone H2B was expressed in E. coli and purified similarly to what was previously

(6) The exquisite specificity of human protein arginine methyltransferase 7 (PRMT7) toward Arg-X-Arg sitesPLOS ONEMay 22, 2023Timothy J. Bondoc, Troy L. Lowe, Steven G. Clarke[9]. Recombinant full-length human histone H2B was purchased from New England Biolabs (NEB catalog no. M2505S). Full-length Xenopus laevis histone H2B was received as a generous gift from Maria Vogelauer and Siavash

(7) The lysine methyltransferase SMYD5 amplifies HIV-1 transcription and is post-transcriptionally upregulated by Tat and USP11Cell reportsMarch 28, 2023Boehm D, Lam V, Schnolzer M, Ott M.,New England Biolabs,Cat# M2502S; Lot# 0131508 "Histone H2B, Human, Recombinant",New England Biolabs,Cat# M2505S; Lot# 0031508 "Histone H3.1, Human, Recombinant",New England Biolabs,Cat# M2503S; Lot# 0041504 "Histone H3.2, Human, Recombinant

(8) Characterization of a polyclonal antibody that is highly selective for the d-isoAsp-25 variant of mammalian histone H2BAmino AcidsJanuary 30, 2023Dana W. Aswad, Kevin S. O’Leary, Katherine Williamsmodification in several mammalian tissues is also presented. Materials and methods Human recombinant H2B (M2505S) was obtained from New England BioLabs (Ipswich, MA). Calf thymus histones (LS002544) were from Worthington Biochemical Corp

(9) Cytotoxic CD8(+) T cells target citrullinated antigens in rheumatoid arthritisNature communicationsJanuary 19, 2023Moon JS, Younis S, Ramadoss NS, Iyer R, Sheth K, Sharpe O, Rao NL, Becart S, Carman JA, James EA, et al.and histones H2B, H3.1 and H4 (Cayman Chemical; 25151, Thermo Fisher; RP43142, New England Biolabs; M2505S, M2503S, M2504S). To block the interaction of HLA class complex and CD8+ T cells, purified anti-human CD8

(10) Human Protein Arginine Methyltransferases (PRMTs) Can Be Optimally Active Under Non-Physiological ConditionsJournal of Biological ChemistrySeptember 1, 2022Troy L. Lowe, Steven G. Clarkeprotein was purified as described for GST-HsPRMT7 above. Human HsH2B was purchased from NEB, Cat. No. M2505S, as a recombinant protein expressed in E. coli, stored in 300 mM NaCl, 1 mM EDTA, and 20 mM sodium phosphate

(11) Induction of broadly reactive influenza antibodies increases susceptibility to autoimmunityCell reportsMarch 8, 2022Labombarde JG, Pillai MR, Wehenkel M, Lin CY, Keating R, Brown SA, Crawford JC, Brice DC, Castellaw AH, Mandarano AH, et al.Biological,Cat# G2006-10 H1 (histone),New England BioLabs,Cat# M2501S H2B (histone),New England BioLabs,Cat# M2505S H3 (histone),New England BioLabs,Cat# M2506S H4 (histone),New England BioLabs,Cat# M2504S Insulin

(12) The macromolecular complexes of histones affect protein arginine methyltransferase activitiesJournal of Biological ChemistryOctober 1, 2021Melody D. Fulton, Mengtong Cao, Meng-Chiao Ho, Xinyang Zhao, Y. George ZhengHMT-21-172, lot no. 1451). Human recombinant full-length histones H2A (catalog no. M2502S), H2B (catalog no. M2505S), H3.3 (catalog no. M2507S), and H4 (catalog no. M2504S) were purchased from New England Biolabs. The

(13) Local states of chromatin compaction at transcription start sites control transcription levelsNucleic Acids ResearchAugust 20, 2021Satoru Ishihara, Yohei Sasagawa, Takeru Kameda, Hayato Yamashita, Mana Umeda, Naoe Kotomura, Masayuki Abe, Yohei Shimono, Itoshi Nikaidosecondary electrophoresis. As a control, recombinant histones H1.0, H3.1, H2B, and H4 (#M2501S, #M2503S, #M2505S and #M2504S, respectively, New England Biolabs) were utilized. Recombinant H1.0 and H3.1, or H2B and

(14) Dual protease type XIII/pepsin digestion offers superior resolution and overlap for the analysis of histone tails by HX-MSMethodsDecember 1, 2020James Mullahoo, Terry Zhang, Karl Clauser, Steven A. Carr, Jacob D. Jaffe, Malvina Papanastasiou(m/z) ratio (MS2). Experimental Section Materials Human recombinant histones (H2A; M2502S, H2B.1; M2505S, H3.1; M2503S and H4; M2504S) were purchased from New England BioLabs Inc (Ipswich, MA). Mononucleosomes,

(15) Functional Interplay between Histone H2B ADP-Ribosylation and Phosphorylation Controls AdipogenesisMolecular cellSeptember 17, 2020Huang D, Camacho CV, Setlem R, Ryu KW, Parameswaran B, Gupta RK, Kraus WL.H2B peptides used for in vitro biochemical assays are listed below. Recombinant H2B proteins (NEB, M2505S) were included at 1 μg per reaction. Assembled recombinant nucleosomes were kindly provided by Dr. Glen

(16) The genomic landscape of metastatic castration-resistant prostate cancers reveals multiple distinct genotypes with potential clinical impactNature CommunicationsDecember 1, 2019Lisanne F. van Dessel et al.immunoprecipitation, human control RNA (4307281; Thermo Fisher Scientific) and recombinant Histone 2B (M2505S; New England Biolabs) were added as carriers, as described previously57. Immunoprecipitated DNA was

(17) Optimized ChIP-seq method facilitates transcription factor profiling in human tumorsLife Science AllianceFebruary 1, 2019Abhishek A Singh et al.immunoprecipitation, human control RNA (4307281; Thermo Fisher Scientific) and recombinant Histone 2B (M2505S; New England Biolabs) were added as carriers. For both cell line and tissue ChIPs, 5 μg of antibody

(18) Elevated H3K79 homocysteinylation causes abnormal gene expression during neural development and subsequent neural tube defectsNature CommunicationsDecember 1, 2018Qin Zhang et al.Supplementary Figure 2G. HTL treatment in vitro Purified histones, including H2a (M2502S, NEB), H2b (M2505S, NEB), H3 (M2503S, NEB) and H4 (M2504S, NEB) were selected. Reactions were carried out at 37 °C in mixtures

(19) Enhancer, transcriptional, and cell fate plasticity precedes intestinal determination during endoderm developmentGenes & DevelopmentNovember 1, 2018Kushal K. Banerjee et al.protease inhibitors and two carriers: 20 µL of recombinant human histone H2B solution (New England Biolabs, M2505S) and 1 µL of carrier RNA from the RNeasy microkit (Qiagen, 74004). Beads were washed five times with

(20) Ubiquitination-Deficient Mutations in Human Piwi Cause Male Infertility by Impairing Histone-to-Protamine Exchange during SpermiogenesisCellJune 1, 2017Gou LT, Kang JY, Dai P, Wang X, Li F, Zhao S, Zhang M, Hua MM, Lu Y, Zhu Y, et al.Histone H2A",New England BioLabs,Cat#M2502S "Human, Recombinant Histone H2B",New England BioLabs,Cat#M2505S Hematoxylin and Eosin,"Shanghai hongqiao lexiang medical reagent technology co., LTD",N/A Dynabeads

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Histone H2B Human, Recombinant

Histone H2B Human, Recombinant from New England Biolabs

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New England Biolabs

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Histone H2B Human, Recombinant from New England Biolabs

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