Fig 1: Impaired G-CSF-induced granulopoiesis in sepsis survivorsSham and CLP mice were administered PBS or G-CSF as in Figure 6.(A) Absolute number of neutrophils (CD11b+Ly6CloLy6G+) in the BM. (B) Frequencies of immature neutrophils (CD101−) and (C) mature neutrophils (CD101+) in the BM. Data represent the mean ± SD from two independent experiments, n = 3–9 mice per group analyzed via two-way ANOVA; ∗p < 0.05, ∗∗∗p < 0.001. (D) Frequencies of immature and mature neutrophils (CD101−/+) in the blood. (E) Representative flow cytometry plots showing the frequency of G-CSFR-positive blood neutrophils. (F) The percentage of G-CSFR+ neutrophils in peripheral blood. Data are pooled from two experiments with n = 3–6 mice/group; two-way ANOVA with Tukey’s multiple comparison test, and two-tailed unpaired Student’s t test for total neutrophils ##p < 0.01. Data represent the mean ± SD with n = 3–6 analyzed via one-way ANOVA, ∗p < 0.05. (G) Concentrations of SOCS3 in BM as determined by ELISA and normalized to total protein. Data represent the mean ± SD from two pooled experiment with n = 5–9 mice per group; analyzed via one-way ANOVA, ∗p < 0.05. (H) Model illustrating the impact of a mobilizing course of G-CSF injections on expansion and mobilization of HSPCs and emergency granulopoiesis sepsis survivors.