STREPTAVIDIN S000-01 from Rockland Immunochemicals, Inc.

Product Specs
ItemSTREPTAVIDIN
CompanyRockland Immunochemicals, Inc.
Price $300.00Supplier Page View Company Product Page
Catalog NumberS000-01
Size5 mg
Activity15.5 U/mg
Conjugate/TagUnconjugated
PurityStreptavidin is chromatographically pure and shows predominantly a single 53,600 dalton band by SDS-PAGE
ApplicationsELISA,Chip,Gel Shift,Western Blot
Concentration0.9 mg/mg
FormatLyophilized
StorageStore vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. Streptavidin is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use

Specifications/Features

Application Note: Streptavidin has been tested by SDS-PAGE and is suitable for use as antigen, as a control or standard in assays, and most other immunological methods as well as enzyme conjugates and complexes; Southern blots and other methodologies related to DNA and RNA analysis; Western blots; and purification of proteins or other antigens with biotinylated antibodies or lectins by use of immobilized streptavidin.

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Rockland Immunochemicals, Inc.

P.O. Box 5199

Limerick, Pennsylvania 19468

United States

Phone: (484) 791-3823
Fax: (484) 369-8654

Company Profile

Website: https://www.rockland.com

(1) Generation of nanobodies with conformational specificity for tau oligomers that recognize tau aggregates from human Alzheimer's disease samplesBiomaterials scienceNovember 19, 2024McArthur N, Squire JD, Onyeachonam OJ, Bhatt NN, Jerez C, Holberton AL, Tessier PM, Wood LB, Kayed R, Kane RS.

(2) Mechanical force regulates ligand binding and function of PD-1Nature CommunicationsSeptember 27, 2024Kaitao Li, Paul Cardenas-Lizana, Jintian Lyu, Anna V. Kellner, Menglan Li, Peiwen Cong, Valencia E. Watson, Zhou Yuan, Eunseon Ahn, Larissa Doudy, et al.single coverslips. Coverslips were washed in 1X PBS and 200 μL of 40 μg/mL streptavidin (Rockland: S000-01) solution was added to each. Streptavidin was incubated on the coverslips for 1 h. Following incubation,

(3) Effects of Salt Concentration on a Magnetic Nanoparticle-Based Aggregation Assay with a Tunable Dynamic RangeSensors (Basel, Switzerland)September 26, 2024Moss G, Knopke C, Diamond SG.

(4) Development of a pan-tau multivalent nanobody that binds tau aggregation motifs and recognizes pathological tau aggregatesBiotechnology progressSeptember 1, 2024McArthur N, Kang B, Rivera Moctezuma FG, Shaikh AT, Loeffler K, Bhatt NN, Kidd M, Zupancic JM, Desai AA, Djeddar N, et al.

(5) Polarized focal adhesion kinase activity within a focal adhesion during cell migrationNature chemical biologyDecember 1, 2023Li X, Combs JD 3rd, Salaita K, Shu X.30 min, washed with 3 ml PBS and then covered with a 50 μg/ml solution of streptavidin(Rockland-Inc. S000-01) in PBS. After incubating the coverslips for 1 hr at room temperature, they were rinsed with 3 ml of

(6) Detection of cellular traction forces via the force-triggered Cas12a-mediated catalytic cleavage of a fluorogenic reporter strandNature biomedical engineeringNovember 1, 2023Duan Y, Szlam F, Hu Y, Chen W, Li R, Ke Y, Sniecinski R, Salaita K.Cyclo[Arg-Gly-Asp-d-Phe-Lys(PEG-PEG)] (PCI-3696-PI) (cRGD) was acquired from Peptides International (Louisville, KY). Streptavidin (S000-01) was purchased from Rockland-Inc (Pottstown, PA). μ-Slide VI0.4 6-channel slides (80606) and 25 mm x

(7) Mechanical force regulates ligand binding and function of PD-1bioRxivAugust 19, 2023Kaitao Li, Paul Cardenas-Lizana, Anna V. Kellner, Zhou Yuan, Eunseon Ahn, Jintian Lyu, Zhenhai Li, Khalid Salaita, Rafi Ahmed, Cheng Zhusingle coverslips. Coverslips were washed in 1X PBS and 200 uL of 40 μg/mL streptavidin (Rockland: S000-01) solution was added to each. Streptavidin was incubated on the coverslips for 1 hour. Following incubation,

(8) DNA Origami Tension Sensors (DOTS) to study T cell receptor mechanics at membrane junctionsbioRxiv : the preprint server for biologyJuly 12, 2023Hu Y, Duan Y, Velusamy A, Narum S, Rogers J, Salaita K.Kingdom). Blebbistatin (Cat# 72402) was purchased from STEMCELL (Vancouver, Canada). Streptavidin (Cat# S000-01) was purchased from Rockland Immunochemicals Inc. (Rockland, NY). Biotinylated pMHC ovalbumin (SIINFEKL)

(9) Progressive enhancement of kinetic proofreading in T cell antigen discrimination from receptor activation to DAG generationeLifeSeptember 20, 2022Britain DM, Town JP, Weiner OD.After incubation, we washed the well with 500 µl TBS before adding 100 µl Streptavidin (Rockland, #S000-01) diluted in TBS-BB (2 µg/ml final) to the well and incubated at room temperature for 5 min. After washing

(10) Author Correction: Turn-key mapping of cell receptor force orientation and magnitude using a commercial structured illumination microscopeNature communicationsSeptember 16, 2021Blanchard A, Combs JD, Brockman JM, Kellner AV, Glazier R, Su H, Bender RL, Bazrafshan AS, Chen W, Quach ME, et al.

(11) Turn-key mapping of cell receptor force orientation and magnitude using a commercial structured illumination microscopeNature communicationsAugust 3, 2021Blanchard A, Combs JD, Brockman JM, Kellner AV, Glazier R, Su H, Bender RL, Bazrafshan AS, Chen W, Quach ME, et al.overnight at 4 °C. The next day, slides were washed in 1× PBS and 64 μg mL−1 of streptavidin (Rockland, S000-01) in PBS was added to each slide for 1 h. Slides were washed again with 1× PBS and 40 μg mL−1 of biotinylated

(12) Sensitivity of Rodent Microglia to Kynurenines in Models of Epilepsy and Inflammation In Vivo and In Vitro: Microglia Activation is Inhibited by Kynurenic Acid and the Synthetic Analogue SZR104International journal of molecular sciencesDecember 7, 2020Lajkó N, Kata D, Szabó M, Mátyás A, Dulka K, Földesi I, Fülöp F, Gulya K, Vécsei L, Mihály A.

(13) Live-cell super-resolved PAINT imaging of piconewton cellular traction forcesNature methodsOctober 1, 2020Brockman JM, Su H, Blanchard AT, Duan Y, Meyer T, Quach ME, Glazier R, Bazrafshan A, Bender RL, Kellner AV, et al.elsewhere abbreviated as cRGD, was acquired from Peptides International (Louisville, KY). Streptavidin (S000–01) was obtained from Rockland-Inc (Pottstown, PA). μ-Slide VI0.4 6-channel slides (80606) and 25 mm ×

(14) Optogenetic Tuning of Protein-protein Binding in Bilayers Using LOVTRAPBIO-PROTOCOLSeptember 5, 2020Doug Tischer, Orion D Weinernumber: H325-500) 39. HCl (Acros, catalog number: 42379-5000) 40. Streptavidin (Rockland, catalog number: S000-01) 41. ProLong Live Antifade Reagent (Thermo Fisher Scientific, catalog number: P36975) 42. Roche cOmplete

(15) Fc Characteristics Mediate Selective Placental Transfer of IgG in HIV-Infected WomenCellJune 13, 2019David R. Martinez, Youyi Fong, Shuk Hang Li, Fang Yang, Madeleine F. Jennewein, Joshua A. Weiner, Erin A. Harrell, Jesse F. Mangold, Ria Goswami, George R. Seage, et al.S1420S SureBlue TMB Microwell Peroxidase Substrate Kit,VWR,Cat# 95059 Streptavidin,Rockland Immunochemicals,Cat# S000–01 "TMB Stop Solution, KPL Inc",VWR,Cat# 95059 TRIzol,ThermoFisher Scientific,Cat# 15596026 Bio-Plex 200

(16) Light-based tuning of ligand half-life supports kinetic proofreading model of T cell signalingeLifeApril 5, 2019Tischer DK, Weiner OD.a well, excess lipids were flushed out with 500 ul 0.22 um filtered dPBS. Streptavidin (Rockland, #S000-01) diluted in dPBS-BB (2 ug/ml final) was flushed into the well and incubated at room temperature for

(17) Mapping the 3D orientation of piconewton integrin traction forcesNature methodsFebruary 1, 2018Brockman JM, Blanchard AT, Pui-Yan V Ma, Derricotte WD, Zhang Y, Fay ME, Lam WA, Evangelista FA, Mattheyses AL, Salaita K.(Product code: 7225–01) were purchased from Electron Microscopy Sciences (Hatfield, PA). Streptavidin (S000–01) was obtained from Rockland-Inc(Pottstown, PA). µ-Slide VI0.4 6 channel slides (80606) and 25 mm × 75

(18) Multiplexed Recombinase Polymerase Amplification Assay To Detect Intestinal ProtozoaAnalytical chemistryFebruary 2, 2016Crannell Z, Castellanos-Gonzalez A, Nair G, Mejia R, White AC, Richards-Kortum R.

(19) An Impedance Aptasensor with Microfluidic Chips for Specific Detection of H5N1 Avian Influenza VirusSensors (Basel, Switzerland)July 29, 2015Lum J, Wang R, Hargis B, Tung S, Bottje W, Lu H, Li Y.

(20) Rheumatoid arthritis-associated autoantibodies in non-rheumatoid arthritis patients with mucosal inflammation: a case-control studyArthritis research & therapyJuly 9, 2015Janssen KM, de Smit MJ, Brouwer E, de Kok FA, Kraan J, Altenburg J, Verheul MK, Trouw LA, van Winkelhoff AJ, Vissink A, et al.

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STREPTAVIDIN

Streptavidin

STREPTAVIDIN from Rockland Immunochemicals, Inc.

Description

Specifications/Features

Rockland Immunochemicals, Inc.

Citations (27)

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STREPTAVIDIN from Rockland Immunochemicals, Inc.

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