Fig 1: Immunohistochemistry of Ang-1 (A–F) and Ang-2 (G–L): Ang-1 and Ang-2 immunolabelling in cerebral cortex (A, D, G, J), hippocampus (B, E, H, K) and microvessels (C, F, I, L) 1 day after SAH. Note decreased Ang-1 staining in SAH (D–F) rats as compared to sham (A–C) rats and increased Ang-2 staining in SAH (J-L) rats as compared to sham (G–I) rats (cortex and hippocampus, ×200; microvessels, ×400). The black arrows indicate endothelial cells. Results are described as mean±standard deviation. Ang-1, angiopoietin-1; Ang-2, angiopoietin-2; 1d pSAH, 1-day post subarachnoid hemorrhage. post SAH. *P<0.05. **P<0.01, versus the sham group.
Fig 2: Evans blue extravasation (A) and neurological scores (B): Evans blue extravasation was evaluated 1 day and neurological scores were recorded 3 days after SAH. Results are described as mean±standard deviation. Ang-1, angiopoietin-1; SAH, subarachnoid hemorrhage. *P<0.05, **P<0.01. NS, not significant, P>0.05 versus the SAH+vehicle group.
Fig 3: Western blot and quantitative analysis of Ang-1 and Ang-2 expression: cerebral cortex (A) and the hippocampus (B), 6-hour (6h), 1-day (1d), 2-day (2d), and 3-day (3d) post SAH. The ratio of Ang-1/Ang-2 after SAH is exhibited in the cortex (C) and hippocampus (D), respectively. Results are described as mean±standard deviation. Ang-1, angiopoietin-1; Ang-2, angiopoietin-2; SAH, subarachnoid hemorrhage; pSAH, post SAH. *P<0.05. **P<0.01, versus the sham group.
Supplier Page from Abcam for Angiopoietin 1 peptide