Fig 1: GC Treatment suppresses osteoclast formation but does not directly regulate ANG gene expression in osteoclasts.a Schematic diagram showing the procedure of the in vitro experiments. Bone marrow mononuclear cells/macrophages were isolated from 3-week-old C57BL/6J mice and cultured with M-CSF and RANKL for 5 days to induce the formation of mature osteoclasts. The cells were treated with vehicle or MPS with or without RU486 for the entire 5 days (i) or only the last day (ii). The cells were subjected to TRAP staining or lysed to measure the mRNA expression level of ANG. TRAP staining of the cells is shown in (b). Quantified number of TRAP+ spreading multinucleated cells/well is shown in (c). The levels of ANG expression were analyzed by qRT-PCR (d). Experiments were performed in triplicate and were repeated three times. Data are represented as mean ± s.e.m. **p < 0.01, ns, not significant as determined by one-way ANOVA with post hoc Tukey test.