Fig 1: Intracellular cytokine expression of Mart-1+ CD8+ T cells cocultured with autologous or artificial APCs.Intracellular cytokine expression of Mart-1 + CD8+ T cells cocultured with autologous mDCs (green box) co-cultured with short and long peptide, and Mart-1 complete protein, and with unstimulated mDCs. They were also co-cultured with HEK293 cells (blue box) transfected with Molecules-GFP-Minigene, GFP-Minigene, GFP only, and untransfected cells. Autologous PBMCs pulsed with MART-1 short peptide were used as positive control (red box), and unstimulated PBMCs as negative control.
Fig 2: Evaluation of Granzyme B expression in CD8+ Mart-1 + co-cultured with transfected HEK293 cells.The population of T CD8+ Mart-1 + cells co-cultured with HEK293 cells transfected with Molecules-GFP-Minigene, GFP-Minigene and GFP only expressing Granzyme B intracellularly assessed by flow cytometry. This was compared with CD8+ Mart-1 + co-cultured with untransfected HEK293 cells.
Fig 3: Expression of IFN-γ + TNF-α + by CD8-Mart-1 + in co-culture with APCs transfected with Minigene and co-stimulatory molecules.A) Tetramer-positive population of T CD8 + Mart-1 + clones compared to unstimulated PBMCs. B) Intracellular cytokine expression of T CD8 + Mart-1 + cells co-cultured with HEK293 transfected with Molecules-GFP-Minigene, with GFP-Minigene, GFP only and untransfected HEK293 cells. Autologous PBMCs pulsed with the short peptide of Melan-A were used as positive control, and unstimulated PBMCs as negative control.
Supplier Page from Abcam for Recombinant Human MelanA protein (GST tag N-Terminus)