Fig 2: LHPP expression in RCC tissue was significantly decreased. A, The Human Protein Atlas project online prediction system was used to evaluate the prognostic value of tissue LHPP in RCC. B,C, Compared with adjacent tissues, the level of LHPP protein in cancer tissues was significantly decreased. T: cancer tissue; N: adjacent tissue. The experiment was performed three times. D, LHPP protein levels in cancer and adjacent tissues of sixty RCC patients. Each point represents an independent sample. E, LHPP mRNA levels in cancer and adjacent tissues of sixty RCC patients. F, Compared with the low LHPP group, the high LHPP group has significantly higher disease-free survival and overall survival. *p < .05, compared with adjacent tissue; **p < .001, compared with adjacent tissue

Fig 3: LHPP is related to the malignant biological behavior in RCC. A, A relatively higher LHPP mRNA expression was detected in the RCC23 cell line. The test was repeated three times for each sample. B, RCC23 cells were transfected with pLV‐LHPP#1, pLV‐LHPP#2, or pLV‐LHPP#3. The test was repeated three times for each sample. C, CCK‐8 assay detected the change of proliferative capacity in the RCC23 cell line after the introduction of pLV‐LHPP. The experiment was performed three times. D, An invasion assay was performed to assess the effect of LHPP on the invasion of RCC23 cells. The experiment was performed three times. E, Western blot results showed that compared with the pLV‐LHPP group, the expression levels of N‐cadherin, β‐catenin, Vimentin, and Bcl‐2 were decreased, while the expression levels of E‐cadherin, Bax, and Caspase‐3 were increased. The experiment was performed three times. *p < .05; **p < .001, compared with HEK‐293T or pLV‐NC