Enterokinase, light chain P8070S from New England Biolabs

Product Specs
ItemEnterokinase, light chain
CompanyNew England Biolabs
Price $131.00 Inquire
Catalog NumberP8070S
Source/Expression SystemSource: This preparation is purified from k. lactis containing a clone of the light chain of the bovine enterokinase gene (1,2).
Molecule NameEnterokinase, light chain
Size480 units
ApplicationsProteomics & Glycomics
Temperature-20 C

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New England Biolabs

240 County Road

Ipswich, Massachusetts 1938

United States

Phone: 800-632-5227
800-632-7799
978-921-1350

Company Profile

Website: http://www.neb.com

(1) Cytoplasmic tail diversity determines the effector bias of the adhesion GPCR ADGRL2Cell Chem BiolMarch 19, 2026Krassimira A. Garbett, Chen Zheng, Julia Drube, Carsten Hoffmann, Vsevolod V. Gurevich, Richard C. SandoTechnologies,Cat#340 TransIT-2020,Mirus,Cat#MIR5400 PEI,Sigma,Cat#408727 Puromycin,Sigma,Cat#P8833 Versene,Gibco,Cat#15040066 Protease Inhibitor Cocktail,Roche,Cat#11873580001 "Enterokinase, light chain",NEB,Cat#P8070S "Blue Protein Standard, Broad Range",NEB,Cat#P7718 In-Fusion Assembly system,Takara,Cat#638948 BCA

(2) Mapping the Mutational Landscape for Streptokinase Binding to PlasminogenbioRxivFebruary 4, 2026Srishti Baid, Matthew L Holding, Taylor Laurin, David Seimeiniak, David Ginsburg, Laura M Haynesbead-bound complexes were then washed with 1×HBS buffer containing BSA. Enteropeptidase (EK) (Product #P8070L, New England Biolabs), which cleaves C-terminal to the FLAG tag, was then added in 50 uL of EK buffer

(3) Dosa: A method to covalently barcode proteins for high-throughput biochemistryProceedings of the National Academy of SciencesJanuary 6, 2026Yashwanth Ashok, Kerry L. Bubb, Curran Oy, Sayeh Gorjifard, Josh T. Cuperus, Christine Queitsch, Stanley Fieldscleavage buffer (20 mM Tris pH 7.5, 50 mM NaCl, 2 mM CaCl2). 5 μL enteropeptidase (Enterokinase [Bovine], #P8070S NEB) was added and incubated at 4 °C overnight with 2 μL murine RNase inhibitor (NEB). The beads were

(4) Structural insights into C3 convertase activity of the classical pathway of complementNature CommunicationsDecember 18, 2025Karla I. De la O Becerra, T. Harma C. Brondijk, Itziar Serna Martin, Piet Grosconcentration of 28 μM was activated by cleavage with 16 units enterokinase (New England Biolabs Inc, P8070L) at 37 °C for 1 h in a total volume of 21 μL in 20 mM HEPES pH 7.4, 150 mM NaCl buffer containing 2

(5) BTG3-dependent VCP/p97 nuclear translocation is required for efficient repair of UV-induced DNA lesionsNucleic Acids ResJuly 8, 2025Jack Dalit Acedera, Yu-Che Cheng, Yi-Ju Li, Hao-Yun Hsueh, Sheau-Yann ShiehHis-VCP-WT and His-VCP-S775A were expressed and purified from E. coli and treated with enterokinase (NEB, P8070S) to remove the His tag at room temperature overnight. Kinase reaction was performed with immunoprecipitated

(6) Cytoplasmic tail composition modulates the G protein and arrestin-3 signaling bias of the adhesion GPCR LPHN2bioRxivJuly 6, 2025Krassimira Garbett, Chen Zheng, Julia Drube, Carsten Hoffmann, Vsevolod V. Gurevich, Richard C. SandoTechnologies,340 TransIT-2020,Mirus,MIR5400 PEI,Sigma,408727 Puromycin,Sigma,P8833 Versene,Gibco,15040066 Protease Inhibitor Cocktail,Roche,11873580001 "Enterokinase, light chain",NEB,P8070S "Blue Protein Standard, Broad Range",NEB,P7718 In-Fusion Assembly system,Takara,638948 BCA Assay

(7) Human Transmembrane Serine Protease 2 (TMPRSS2) on Human Seminal Fluid Extracellular Vesicles Is Proteolytically ActiveJ Extracell VesiclesMarch 16, 2025Emile Verhulst, Michelle De Bruyn, Pascale Berckmans, Yani Sim, Koen Augustyns, Isabel Pintelon, Maya Berg, Pieter Van Wielendaele, Anne-Marie Lambeir, Yann G.‐J. Sterckx, et al.divided into 49 µL aliquots, to which 1 µL enterokinase (16,000 U/mL, New England Biolabs, Cat. No. P8070) was added for overnight TMPRSS2 zymogen activation in the dark at 16°C. To remove enterokinase, the

(8) Molecular basis of promiscuous chemokine binding and structural mimicry at the C-X-C chemokine receptor, CXCR2Molecular CellMarch 6, 2025Shirsha Saha, Fumiya K. Sano, Saloni Sharma, Manisankar Ganguly, Sudha Mishra, Annu Dalal, Hiroaki Akasaka, Takaaki A. Kobayashi, Nashrah Zaidi, Divyanshu Tiwari, et al.was set up to remove the 6X-His-tag by incubating with either homemade or store bought (NEB, Cat. no: P8070L) enterokinase in the presence of 10mM CaCl2 at 22°C for 16h. Cleaved protein was then loaded onto the

(9) The Adhesion GPCR ADGRL2 engages Gα13 to Enable Epidermal DifferentiationbioRxivMarch 1, 2025Xue Yang, Feng He, Douglas F. Porter, Krassimira Garbett, Robin M. Meyers, David L. Reynolds, Duy Lan Huong Huong Bui, Audrey Hong, Luca Ducoli, Zurab Siprashvili, et al.Scientific,15240–062 Stellar Competent Cells,Takara Bio,636763 Thrombin,Sigma,T4846 "Enterokinase, light chain",NEB,P8070S SPRIselect beads,Beckman Coulter,B23317 RIPA Lysis Buffer,Thermo Fisher Scientific,89900 "cOmplete™,

(10) Acute stress and multicellular development alter the solubility of the Dictyostelium Sup35 ortholog ERF3Microbiology SpectrumNovember 5, 2024Felicia N. Williams, Kanesha L. Travis, Holly N. Haver, Anna D. Umano, Yaneli Guerra-Hernandez, K. Matthew ScaglioneµM, and the specified recombinant protein was added to a concentration of 15 µM. Enterokinase (NEB #P8070L) was added at 1.6 units per 50 µL reaction to initiate aggregation. Each reaction was then added to

(11) Production of active human iduronate-2-sulfatase (IDS) enzyme in Nicotiana benthamianaScientific reportsOctober 4, 2024Sinha MH, Mehtab T, Entesari M, Nguyen HH, Yun A, Hwang I.50 mM NaCl, and 2 mM CaCl2) and then incubated with 10 µL (50 U) enterokinase light chain (Cat. No. P8070, New England Biolabs, USA) at 25oC for 16 h under gentle rotation at 150 rpm according to the manufacturer’s

(12) A CD25-biased interleukin-2 for autoimmune therapy engineered via a semi-synthetic organismCommun Med (Lond)March 26, 2024Ptacin JL, Ma L, Caffaro CE, Acuff NV, Germar K, Severy P, Qu Y, Vela JL, Cai X, San Jose KM, et al.collected. Samples were supplemented with 0.5 µl enterokinase (New England Biolabs, Ipswich, MA, Cat. No. P8070L) per 10 µg of protein and incubated overnight at room temperature. To initiate pegylation, 5 mM DBCO-mPEG

(13) Distinct evolution of type I glutamine synthetase in Plasmodium and its species-specific requirementNature CommunicationsJuly 14, 2023Sourav Ghosh, Rajib Kundu, Manjunatha Chandana, Rahul Das, Aditya Anand, Subhashree Beura, Ruchir Chandrakant Bobde, Vishal Jain, Sowmya Ramakant Prabhu, Prativa Kumari Behera, et al.recombinant proteins was also verified after cleaving the 6xHis tag with enterokinase (New England Biolabs, P8070S). For enzyme assays carried out with parasite lysates of Pf or Pb, parasite pellets isolated by saponin

(14) Antibody-guided proteases enable selective and catalytic degradation of challenging therapeutic targetsThe Journal of biological chemistryMay 1, 2023Romei MG, Leonard B, Kim I, Kim HS, Lazar GA.Hepes, 150 mM NaCl, and 10 mM CaCl2 at pH 7.5). MMP3-D4K-4 was activated with 16 units of EK (NEB, P8070L) for every 25 μg protein through incubation at room temperature for 16 h. To inactivate the EK, 0.1

(15) A bio-orthogonal linear ubiquitin probe identifies STAT3 as a direct substrate of OTULIN in glioblastomaNucleic acids researchFebruary 22, 2023Du X, Pang J, Gu B, Si T, Chang Y, Li T, Wu M, Wang Z, Wang Y, Feng J, et al.Invitrogen), Pierce™ High Capacity Streptavidin Agarose (20359, Thermo Fisher), enterokinase (NEB, P8070s), enzyme-linked chemiluminescence (ECL) plus (32132, Thermo Fisher), Opti-MEM™ (31985070, Gibco), Sosoo

(16) A novel broad spectrum venom metalloproteinase autoinhibitor in the rattlesnake Crotalus atrox evolved via a shift in paralog functionProceedings of the National Academy of SciencesDecember 20, 2022Fiona P. Ukken, Noah L. Dowell, Mamta Hajra, Sean B. CarrollmM Tris, pH 8.0, 50 mM NaCl, and 2 mM CaCl2) and digested with EK light chain (New England Biolabs, P8070L) at a concentration of 0.2 U/µg of recombinant protein. The reaction mix was incubated at 25 °C for

(17) Recombinant Expression in Pichia pastoris System of Three Potent Kv1.3 Channel Blockers: Vm24, Anuroctoxin, and Ts6Journal of FungiNovember 17, 2022Jesús Borrego, Muhammad Umair Naseem, Al Nasar Ahmed Sehgal, Lipsa Rani Panda, Kashmala Shakeel, Attila Gaspar, Cynthia Nagy, Zoltan Varga, Gyorgy Panyiand Ts6 was cleaved with enterokinase light chain (New England, Biolabs, Ipswich, MA, USA, Cat. No. P8070L). The reaction mixture was prepared as follows. Twenty-five micrograms of peptide was mixed with 1 μL

(18) Binding of the SARS-CoV-2 envelope E protein to human BRD4 is essential for infectionStructureSeptember 1, 2022Kendra R. Vann et al.peptides,Synpeptide,N/A PreScission protease,Home expressed,N/A Thrombin protease,MP Biomedicals,154163 Enterokinase protease,NEB,P8070S Hoechst 33258,Invitrogen,Cat # H3570 Cell Mask,Invitrogen,Cat # C10046 Recombinant p300 (catalytic

(19) Structural basis for binding diversity of acetyltransferase p300 to the nucleosomeiScienceJuly 15, 2022Suguru Hatazawa, Jiuyang Liu, Yoshimasa Takizawa, Mohamad Zandian, Lumi Negishi, Tatiana G. Kutateladze, Hitoshi Kurumizaka(K1456A/K1459A/K1461A/R1462A),This paper,N/A Thrombin,Cytiva,Cat#27084601 "Enterokinase, light chain",New England Biolabs,Cat#P8070L Pre-Scission protease,Zhang et al. (2018),N/A "Glutaraldehyde 25% solution, practical grade",Electron

(20) Mutation of Methionine to Asparagine but Not Leucine in Parathyroid Hormone Mimics the Loss of Biological Function upon OxidationBiochemistryJune 7, 2022Amit Gaur, Jessica Lipponen, Yanmei Yang, Roger S. Armen, Bin Wang12361010) for site-directed mutagenesis was purchased from Thermo Fisher. Enterokinase enzyme (Cat. No. P8070), Phusion polymerase (Cat. No. M0530S), DpnI (Cat. No. R0176S), KpnI (Cat. No. R0142S), XhoI (Cat. No.

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Enterokinase, light chain

Enterokinase, light chain from New England Biolabs

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New England Biolabs

Citations (33)

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Enterokinase, light chain from New England Biolabs

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