Deoxynucleotide (dNTP) Solution Set N0446S from New England Biolabs

Product Specs
ItemDeoxynucleotide (dNTP) Solution Set
CompanyNew England Biolabs
Price $189.00 Inquire
Catalog NumberN0446S
Size25 µmol
Molecule NameDeoxynucleotide
ApplicationsDNA Analysis
Temperature-20 C

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New England Biolabs

240 County Road

Ipswich, Massachusetts 1938

United States

Phone: 800-632-5227
800-632-7799
978-921-1350

Company Profile

Website: http://www.neb.com

(1) sc-rDSeq: a robust and cost-effective full-length total RNA sequencing method for single cells reveals multilayered heterogeneity in drug-resistant lung cancer cellsNucleic Acids ResApril 13, 2026Xue Sun, Shir-Liya Dadon, Dena Ennis, Wenpeng Fan, Muhammad Awawdy, Eli Reuveni, Adi Alajem, Oren RamDithiothreitol (DTT), 9 μl 10% (v/v) IGEPAL CA-630 (Sigma–Aldrich, cat #I8896), 6 μl 25 mM dNTPs (NEB, cat #N0446S) and 55 μl nuclease-free water. (iv) Carrier oil inlet: The carrier oil was 3 ml of HFE-7500 with 2%

(2) Analysis and control of untemplated DNA polymerase activity for guided synthesis of kilobase-scale DNA sequencesNature CommunicationsFebruary 26, 2026Simeon. D. Castle, Thea C. T. Irvine, Adrian Woolfson, Gregory Linshiz, Blake T. Riley, Ifor D. W. Samuel, Loren Picco, Philipp Holliger, Lauren M. Oldfield, Andrew Hessel, et al.Biolabs, N0447S; or combinations of single types of dNTP supplied as a separate set, New England Biolabs, N0446S), 1 µL Taq polymerase (2 units final concentration), 2 µL ROX solution (ThermoFisher Scientific, R1371),

(3) Toehold-VISTA: a machine learning approach to decipher programmable RNA sensor-target interactionsNucleic Acids ResFebruary 16, 2026James M Robson, Alexander A GreenM0208L), and 7 ng/µl ET SSB (New England Biolabs M2401S) in Gibson buffer [1 mM dNTPs (New England Biolabs N0446S), 5 mM NAD+ (New England Biolabs B9007S), 500 mM Tris–HCl (VWR 101641–844), 50 mM MgCl2 (Sigma 63069),

(4) Integrative Single-Cell Epigenomic Atlas Annotates the Regulatory Genome of the Adult Mouse BrainbioRxivFebruary 7, 2026Zhaoning Wang, Songpeng Zu, Ethan J. Armand, Timothy H. Loe, Jonathan A. Rink, Wanying Wu, Yang Xie, Lei Chang, Chenxu Zhu, Nicholas D. Johnson, et al.each 25 μL purified sublibrary from the previous step, 3 μL 10x TdT Buffer and 1 μL 1 mM dCTP (NEB, N0446S) were added and the reaction was incubated at 95°C for 5 min, and then immediately chilled on ice for

(5) Neural stem cell quiescence is actively maintained by the epigenomeCell ReportsJanuary 27, 2026Anna Malkowska, Jan Ander, Andrea H. BrandMagnetic Carboxylate,Cytiva,65152105050250 100% ethanol,–,– Deoxynucleotide (dNTP) Solution Set,NEB,N0446S 16% Formaldehyde Solution (w/v),ThermoFisher Scientific,28908 Triton X-100,Sigma Aldrich,T9284 Vectashield

(6) Predictive prioritization of enhancers associated with pancreatic disease riskCell GenomJanuary 14, 2026Wang L, Baek S, Prasad G, Wildenthal J, Guo K, Sturgill D, Truongvo T, Char E, Pegoraro G, McKinnon K, et al.heat-inactivated at 62°C for 20 min. To label ends of the digested chromatin fragments, 14 μL of dNTP (NEB N0446S) (dATP-14-biotin [Axxora, NU-835-BIO14-S] and dTTP, dCTP, dGTP at a final concentration of 40 μM each)

(7) Pathways and products of base excision DNA repair in Xenopus laevis eggs: contrast with human cell pathwaysNucleic Acids ResDecember 10, 2025Zhouyiyuan Xue, Patrick James Sutton, John D Haley, Christopher W Brownlee, Bruce Demple20 min. For the blunting step, nine units of T4 DNA polymerase (NEB, M0203S), 5 nmol of dNTPs (NEB, N0446S), and 1 μl of 10× NEB buffer r2.1 (NEB, B6002S) were added to the reaction mixture, with H2O used to

(8) Renal clearable CRISPR nanosensor targeting mitochondrial DNA mutation for noninvasive monitoring of tumor progression and metastasisScience AdvancesNovember 21, 2025Yanan Li, Yonghua Wu, Ziyue Zheng, Yige Wu, Yongyuan Zhang, Jingjing Zhang, Fenglei Quan, Wen Zhao, Ru Xu, Yiwei Li, et al.Transcription Kit (catalog no. E131, Novoprotein, Shanghai, China) was used, and the deoxyribonucleotide set (N0446S, New England Biolabs) was applied for DNF preparation. The Mitochondrial Permeability Transformation

(9) Coalescing single-cell genomes and transcriptomes to decode breast cancer progressionCellOctober 30, 2025Kaile Wang, Rui Ye, Shanshan Bai, Zhenna Xiao, Lei Yang, Jianzhuo Li, Chenling Tang, Emi Sei, Jinyu Peng, Anna K. Casasent, et al.incubated 20 min at 55°C and then stopped by dispensing 35 nl neutralization mix containing 7.8mM dCTPs (N0446S, NEB), 3.9 mM dATPs (N0446S, NEB), 3.9 mM dGTPs (N0446S, NEB), 3.9 mM dTTPs (N0446S, NEB), 1.5 U/µl

(10) Engineering wetware and software for the predictive design of compressed genetic circuits for higher-state decision-makingNat CommunOctober 24, 2025Brian D. Huang, Yongjoon Yu, Junghwan Lee, Matthew W. Repasky, Yao Xie, Matthew J. Realff, Corey J. Wilsonmutation using the following formula: 5 μL Mg-free Taq buffer (NEB B9015S), 4 μL of 1 mM dATP (NEB N0446S), 4 μL of 1 mM dGTP (NEB N0446S), 4 μL of 5 mM dTTP (NEB N0446S), 4 μL of 5 mM dCTP (NEB N0446S), 3.6

(11) Replication competition drives the selective mtDNA inheritance in Drosophila ovaryCell ReportsSeptember 23, 2025Cheng Zhang, Zhe Chen, Hansong Ma, Hong XuMedical,Cat# RGF-3240 Salmon sperm DNA,Invitrogen,Cat# 15-632-011 dNTP Solution Set,New England Biolabs,Cat# N0446S Agarose,Lonza Bioscience,Cat# 50004 TAE Buffer,Quality Biological,Cat# 351-008-491 CF®405M Phalloidin,Biotium Inc

(12) An FGFR-p53 developmental signaling axis drives salivary cancer progressionOncogeneAugust 25, 2025Adele M. Musicant, Julia M. R. Billington, Jeffrey S. Damrauer, Jennifer L. Modliszewski, Luane J. B. Landau, Yi-Hsuan Tsai, Jay H. Mehta, John Powers, Renee Betancourt, Radhika Sekhri, et al.1 to 4 μg of RNA using SuperScript IV Reverse Transcriptase (Invitrogen #18090050) with dNTPs (NEB #N0446S), RNase inhibitor (Applied Biosystems #N808–0119), 25 μM oligo d(T)20 primer (Invitrogen #100023441),

(13) Toehold-VISTA: A machine learning approach to decipher programmable RNA sensor-target interactionsbioRxivAugust 13, 2025James M. Robson, Alexander A. GreenM0208L), and 7 ng/μL ET SSB (New England Biolabs M2401S) in Gibson buffer [1 mM dNTPs (New England Biolabs N0446S), 5 mM NAD+ (New England Biolabs B9007S), 500 mM Tris-HCl (VWR 101641–844), 50 mM MgCl₂ (Sigma 63069),

(14) Visualizing DNA polymerase ι catalyze Hoogsteen-directed DNA synthesisNat CommunJuly 1, 2025Zach Frevert, Devin T. Reusch, Melissa S. Gildenberg, Sarah M. Jordan, Benjamin J. Ryan, Bret D. Freudenthal, M. Todd Washingtonabove. Crystallization Pol ι (20 mg/ml), the DNA substrate (500 μM), and dTTP (1 mM, New England Biolabs, catalog number N0446S) were co-crystallized in the presence of 5 mM CaCl2. The best diffracting crystals were obtained using

(15) Development of circular AAV cargos for targeted seamless insertion with large serine integrasesMolecular Therapy Methods & Clinical DevelopmentJune 12, 2025Brett J.G. Estes, Nisha Gandhi, Jessica R. Von Stetina, Dev Paudel, Angela X. Nan, Parth Amin, Joshua Rose, Shuai Wu, Kangni Zheng, Yijun Zhang, et al.and 2 pmol of each purified atgRNA 2 days after plating. Media was supplemented with dTTP/dGTP (NEB N0446S) at a final concentration of 200 μM (equimolar) in the well. For I-PGI experiments with HDAd cargo,

(16) TAC-C uncovers open chromatin interaction in crops and SPL-mediated photosynthesis regulationSci AdvMay 30, 2025Jingmin Kang, Zhaoheng Zhang, Xuelei Lin, Fuyan Liu, Yali Song, Peng Zhao, Yujing Lin, Xumei Luo, Xiaoyi Li, Yanyan Yang, et al.μl of Biotin-14-dCTP (Invitrogen, 19518018), 1.5 μl of 10 mM 2′-deoxyadenosine 5′-triphosphate (NEB, N0446S), 1.5 μl 10 mM 3′-deoxythymidine 5′-triphosphate (NEB, N0446S), 1.5 μl of 10 mM 2′-deoxyguanosine 5′-triphosphate

(17) A single amino acid mutation alters multiple neutralization epitopes in the respiratory syncytial virus fusion glycoproteinNpj VirusesApril 22, 2025Ahmed K. Oraby, Aleksandra Stojic, Farah Elawar, Leanne M. Bilawchuk, Ryley D. McClelland, Kaci Erwin, Madison J. Granoski, Cameron D. Griffiths, Justin D. Frederick, Elena Arutyunova, et al.fragment was created separately using the Q5Ⓡ High-Fidelity DNA polymerase protocol. Briefly, dNTPs (NEB, N0446S), template plasmid RSV RW30 DNA, forward and reverse primers, Q5Ⓡ Reaction Buffer (NEB, B9027S) and

(18) Systemic genome-epigenome analysis captures a lineage-specific super-enhancer for MYB in gastrointestinal adenocarcinomaMolecular Systems BiologyApril 15, 2025Fuyuan Li, Shangzi Wang, Lian Chen, Ning Jiang, Xingdong Chen, Jin Li,New England Biolabs,M0493L Q5 Site-Directed Mutagenesis kit,New England Biolabs,E0554S 10 mM dNTPs,New England Biolabs,N0446S NEBuffer™ 2,New England Biolabs,B7002S EcoRI-HF®,New England Biolabs,R3101L AgeI-HF®,New England Biolabs

(19) Widespread variation in molecular interactions and regulatory properties among transcription factor isoformsMolecular CellApril 3, 2025Luke Lambourne, Kaia Mattioli, Clarissa Santoso, Gloria Sheynkman, Sachi Inukai, Babita Kaundal, Anna Berenson, Kerstin Spirohn-Fitzgerald, Anukana Bhattacharjee, Elisabeth Rothman, et al.Thermo Sequenase DNA Polymerase (with Thermoplasma acidophilum Inorganic Pyrophosphatase (TAP)),Cytiva Life Sciences,E790000Y "Deoxynucleotide (dNTP) Solution Set, 100mM",New England BioLabs,N0446S Amersham CyDye Fluorescent Nucleotides Cy3-dUTP,Cytiva Life Sciences,PA53022 Triton X-100,Sigma-Aldrich,T9284 Nonfat

(20) CCCTC-binding factor N-terminal domain regulates clustered protocadherin gene expression by enhancing cohesin processivityJournal of Biological ChemistryApril 1, 2025Yijun Zhang, Qiang WuDNA Polymerase I, Large (Klenow) Fragment (NEB M0210S), biotin-14-dATP (Thermo 19524016), as well as dCTP, dTTP, and dGTP (NEB N0446S). The reaction was then incubated at 37 °C for 1 h. After labeling the ends of restriction fragments,

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Deoxynucleotide (dNTP) Solution Set

Deoxynucleotide (dNTP) Solution Set from New England Biolabs

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New England Biolabs

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Deoxynucleotide (dNTP) Solution Set from New England Biolabs

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