Deoxynucleotide (dNTP) Solution Set N0446S from New England Biolabs

Product Specs
ItemDeoxynucleotide (dNTP) Solution Set
CompanyNew England Biolabs
Price Pricing Info Supplier Page View Company Product Page
Catalog NumberN0446S
Quantity25 µmol
Molecule NameDeoxynucleotide
ApplicationsDNA Analysis
Brand/TrademarkNew England Biolabs Inc (NEB)
Temperature-20 C

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New England Biolabs

240 County Road

Ipswich, Massachusetts 1938

United States

Phone: 800-632-5227
800-632-7799
978-921-1350

Company Profile

Website: http://www.neb.com

(1) Simultaneous and rapid colorimetric detection of distinct miRNAs using Split-LAMPFrontiers in Bioengineering and BiotechnologyNovember 2, 2023Yi Jing Chua, Steven Poh Chuen Sim, Medha Shridharan, Yiqi SeowComponents,Concentrations "dNTPs (catalog number N0446S, New England Biolabs)",1.4 mM "EvaGreen (catalog number #31000, Biotium)",0.5X "pH responsive buffer

(2) Enzymatic synthesis and nanopore sequencing of 12-letter supernumerary DNANature CommunicationsOctober 26, 2023Hinako Kawabe, Christopher A. Thomas, Shuichi Hoshika, Myong-Jung Kim, Myong-Sang Kim, Logan Miessner, Nicholas Kaplan, Jonathan M. Craig, Jens H. Gundlach, Andrew H. Laszlo, et al.NEBNext® Quick Ligation Module (E6056S), and 2′-Deoxynucleoside triphosphates (dNTPs; N = A, T, G, C; N0446S) were purchased from New England Biolabs (Ipswich, MA). Gene ruler 1 kb plus DNA ladder (SM1331) was

(3) Ribonucleotide synthesis by NME6 fuels mitochondrial gene expressionThe EMBO journalSeptember 18, 2023Grotehans N, McGarry L, Nolte H, Xavier V, Kroker M, Narbona-Pérez ÁJ, Deshwal S, Giavalisco P, Langer T, MacVicar T.FBS,Gibco,26400‐044 Galactose,Serva,22020.02 Glucose,Sigma,G7021 Glutamine,Gibco,25030‐081 Sodium Pyruvate,Gibco,11360 rNTPs,New England BioLabs,N0466S dNTPs,New England BioLabs,N0446S EmbryoMax 100× Nucleosides,Millipore,ES‐008‐D Adenosine,Sigma,A4036 Guanosine,Sigma,G6264 Cytidine,Sigma,C4654 Uridine,Sigma,U3003 Lipofectamine

(4) Protocol for the use of signal amplification by exchange reaction-fluorescence in situ hybridization on adult formalin-fixed paraffin-embedded mouse lung tissueSTAR ProtocolsJune 9, 2023Rachel Warren, Aftab Shaik, Lauren Teubner, Handeng Lyu, Stijn De Langhe(UltraPure DNAse, RNAse free distilled water)",Invitrogen,Cat# 10977-015 dNTPs,New England Biolabs,Cat# N0446S 10% Tween-20 (Surfact-Amps),Thermo Scientific,Cat# 28320 10x PBS,Bio-Rad,Cat# 1610780 1x PBS,MilliporeSigma,Cat#

(5) Targeted Demethylation of the TGFβ1 mRNA Promotes Myoblast Proliferation via Activating the SMAD2 Signaling PathwayCellsMarch 24, 2023Kaiping Deng, Zhipeng Liu, Xiaodan Li, Zhen Zhang, Yixuan Fan, Qunhao Huang, Yanli Zhang, Feng WangBriefly, 1.5 µg of total RNA was mixed with 40 nM up primer, 40 nM down primer, and 5 µM dNTP (NEB, #N0446S) in 17 µL 1× CutSmart buffer (NEB, #B7204S), and then was incubated at 90 °C, 80 °C, 70 °C, 60 °C, and

(6) Optimized protocol for live imaging of overnutrition-elicited interactions between immune cells and β cells in zebrafishSTAR ProtocolsMarch 1, 2023Bingyuan Yang, Yue Zhang, Liu Yang, Wenbiao Chenhydrochloride solution,Millipore Sigma,Cat# T2819 Deoxynucleotide (dNTP) Solution Set,New England Biolabs,N0446S GoTaq® DNA Polymerase,Promega,Cat# M3001 ,, Oligonucleotides,Oligonucleotides,Oligonucleotides ,, tnfa

(7) Pseudomonas-specific 16S rRNA insect gut-microbiome profiling using next-generation sequencingSTAR ProtocolsMarch 1, 2023Ayushi Gupta, Suresh Nairand recombinant proteins" ,, Deoxynucleotide (dNTPs) Solution Mix,"New England BioLabs, USA",Cat#N0446S Taq DNA polymerase,"Bangalore GeNei, India",Cat#0601600051730 Imidacloprid (Confidor 17.80% SL),"Bayer

(8) T7Max transcription systemJournal of Biological EngineeringJanuary 23, 2023Christopher Deich, Brock Cash, Wakana Sato, Judee Sharon, Lauren Aufdembrink, Nathaniel J. Gaut, Joseph Heili, Kaitlin Stokes, Aaron E. Engelhart, Katarzyna P. AdamalaBioReady Taq DNA Polymerase (BSA12L010) was used according to manufacturer’s instructions with NEB dNTPs (N0446S). 9μL of PCR master mix reagents and anti-sense primer were combined with 1 μL of the promoter primer

(9) In vivo, genome-wide profiling of endogenously tagged chromatin-binding proteins with spatial and temporal resolution using NanoDam in DrosophilaSTAR ProtocDecember 16, 2022Jocelyn L.Y. Tang, Robert Krautz, Oriol LlorÃ -Batlle, Anna E. Hakes, Paul M. Fox, Andrea H. Brandbeads, 1 μM, 15 mL)",Fisher Scientific,Cat#09-981-123 Deoxynucleotide (dNTP) Solution Set,NEB,Cat#N0446S DpnI (+ CutSmart Buffer),NEB,Cat#R0176 DpnII (+DpnII Buffer),NEB,Cat#R0543S EDTA,Sigma-Aldrich,Cat#E6758 Klenow

(10) Identification of spermatogenesis-associated changes in DNA methylation induced by maternal exposure to chemicals in male germ cellsSTAR ProtocolsDecember 16, 2022Yukiko Tando, Yasuhisa MatsuiGene,Cat#312-01791 10× NEBuffer 2,NEB,Cat#B7002S MspI,NEB,Cat#R0106S Deoxynucleotide solution set,NEB,Cat#N0446S Klenow Fragment (3′-5′ exo-),NEB,Cat#M0212S High Concentration T4 DNA Ligase,NEB,Cat#M0202M 10× T4 DNA Ligase Reaction Buffer

(11) Development of an optimized colorimetric RT-LAMP for SARS-CoV-2 assay with enhanced procedure controls for remote diagnosticsScientific ReportsDecember 11, 2022Bruna Winkert Raddatz, Edson Yu Sin Kim, Louise Matiê Imamura, Gisleine Jarenko Steil, Erika Bergamo Santiago, Santiago Pedro Timm Soares, Victor Henrique Alves Ribeiro, Bernardo Montesanti Machado de Almeida, Sergio Renato Rogal, Marcus Vinícius Mazega FigueredoAntarctic Thermolabile UDG (M0372), WarmStart® RTx Reverse Transcriptase (M0380), as well as dNTPs (N0446S and N0459S) were acquired from New England Biolabs (NEB). Phenol Red (114529), Mineral Oil (M5904),

(12) Temporal analysis suggests a reciprocal relationship between 3D chromatin structure and transcriptionCell ReportsNovember 1, 2022Kathleen S.M. Reed, Eric S. Davis, Marielle L. Bond, Alan Cabrera, Eliza Thulson, Ivana Yoseli Yoseli Quiroga, Shannon Cassel, Kamisha T. Woolery, Isaac Hilton, Hyejung Won, et al.A63881 Dynabeads MyOne Streptavidin T1 beads,ThermoFisher,Cat# 65601 100 mM dNTP Solution Set,NEB,Cat# N0446S NEB T4 Polynucleotide kinase (PNK),NEB,Cat# M0201S NEB T4 DNA polymerase I,NEB,Cat# M0203S NEB Klenow

(13) Light-Seq: light-directed in situ barcoding of biomolecules in fixed cells and tissues for spatially indexed sequencingNature methodsNovember 1, 2022Kishi JY, Liu N, West ER, Sheng K, Jordanides JJ, Serrata M, Cepko CL, Saka SK, Yin P.with an A-tailing master mix consisting of 1× ThermoPol Buffer (NEB cat. no. B9004S), 1 mM dATP (NEB N0446S), 25 µM ddATP (Sigma GE27-2051-01) and 1,000 U ml−1 terminal transferase enzyme (NEB M0315L) for a 45-min

(14) Safe, Effective, and Inexpensive Clearance of Mycoplasma Contamination from Cultures of Apicomplexan Parasites with SparfloxacinMicrobiology SpectrumOctober 26, 2022Darlene R. Malave-Ramos, Kit Kennedy, Melanie N. Key, Zhicheng Dou, BjÃ¶rn F. C. Kafsackreaction buffer (NEB; M0273S), a nucleotide mix containing 10 mM each dTTP, dATP, dGTP, and dCTP (NEB; N0446S), and 100 fg of the plasmid pUC18. pUC18 can be substituted with any other pBlueScript II-derived plasmid.

(15) High-resolution phylogenetic and population genetic analysis of microbial communities with RoC-ITSISME CommunicationsOctober 10, 2022Douglas B. Rusch, Jie Huang, Chris Hemmerich, Matthew W. Hahnµl at 30 °C overnight with 2 µl of circulated DNA with 2.5 μL of 10 μM (each) dNTPs (Catalog number: N0446S Vendor: New England Biolabs Inc), 2.5 μL Rolling cycle primer (10 μM), 37 μL ultrapure water, 5 μL of

(16) Rapid and sensitive single-cell RNA sequencing with SHERRY2BMC biologySeptember 30, 2022Di L, Liu B, Lyu Y, Zhao S, Pang Y, Zhang C, Wang J, Qi H, Shen J, Huang Y.containing 1× phi29 reaction buffer (NEB, M0269S), 20 μM random primers (Thermo, SO181), and 1 mM dNTP (NEB, N0446S, and N0459S), then they were incubated at 98 °C for 3 min and immediately cooled down at 4 °C for 20

(17) RIPOR2-mediated autophagy dysfunction is critical for aminoglycoside-induced hearing lossDevelopmental cellSeptember 26, 2022Li J, Liu C, Müller U, Zhao B.Thermo Pol Buffer,New England Biolabs,Cat# M0267X Deoxynucleotide Solution,New England Biolabs,Cat# N0446S Platinum Pfx DNA Polymerase,ThermoFisher Sci,Cat# 11708013 HBSS,ThermoFisher Sci,Cat# 14175103 DMEM,ThermoFisher

(18) A Tool to Design Bridging Oligos Used to Detect Pseudouridylation Sites on RNA after CMC TreatmentNon-Coding RNASeptember 23, 2022Baptiste Bogard, Gilles Tellier, Claire Francastel, Florent HubÃ©(Thermo Scientific, cat. no. SO142)Deoxynucleotide (dNTP) Solution Set (New England BioLabs, cat. no. N0446S)18s RT primer: 5′-ATCCGAGGGCCTCACTAAAC-3′RNase H, recombinant (New England BioLabs, cat. no. M0297L)Adaptor:

(19) Aging is associated with increased chromatin accessibility and reduced polymerase pausing in liverMolecular Systems BiologySeptember 9, 2022Mihaela Bozukova, Chrysa Nikopoulou, Niklas Kleinenkuhnen, Dora Grbavac, Katrin Goetsch, Peter TessarzPolynucleotide Kinase (PNK),NEB,M0201S Quick Ligation™ Kit,NEB,M2200S Deoxynucleotide Solution Set,NEB,N0446S RNase A,Thermo Fischer,EN0531 proteinase K,Thermo Fischer,EO0491 AMPure XP beads,Beckman Coulter,A63881 pAG‐Mnase

(20) α-synucleinopathy exerts sex-dimorphic effects on the multipurpose DNA repair/redox protein APE1 in mice and humansProg NeurobiolSeptember 1, 2022Miner KM, Jamenis AS, Bhatia TN, Clark RN, Rajasundaram D, Sauvaigo S, Mason DM, Posimo JM, Abraham N, DeMarco BA, et al.the PANT reaction mixture containing dGTP, dCTP, and dTTP at 30 μM each, 1 μM dATP (dNTP Solution Set N0446S, New England BioLabs, Ipswich, MA), 29 μM biotinylated dATP (19524016, Thermo Fisher Scientific, Pittsburgh,

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Deoxynucleotide (dNTP) Solution Set

Deoxynucleotide (dNTP) Solution Set from New England Biolabs

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New England Biolabs

Citations (103)

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Deoxynucleotide (dNTP) Solution Set from New England Biolabs

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