Human M-CSF, premium grade 130-096-485 from Miltenyi Biotec

(1) Glycosaminoglycan-functionalized hydrogels for sustained delivery of tissue inhibitor of metalloproteinase-3 mediating matrix metalloprotease inhibition and extracellular matrix stabilizationBioactive MaterialsJuly 1, 2026Fabian Junker, Stefan Rupf, Paula Marie Schindler, Cedric Wilden, Mathias Hohl, Gloria Ruiz-Gómez, M. Teresa Pisabarro, Selina Wrublewsky, Caroline Bickelmann, Charlotte Berhorst, et al.cells or in presence of human GM-CSF (25 ng/mL, Milteyni # 130-093-865) or M-CSF (50 ng/ml, Miltenyi # 130-096-489) for polarization into M1 or M2 macrophages, respectively. At the end of the induction phase, cells

(2) Alignment of in vitro and in vivo pulmonary inflammation models using crystalline quartz silicaParticle and Fibre ToxicologyFebruary 19, 2026Isidora Loncarevic, Seyran Mutlu, Martina Dzepic, Sandeep Keshavan, Sandor Balog, Alke Petri-Fink, Fabian Blank, Barbara Rothen-Rutishauser(cRPMI). For MDM differentiation, 10 ng/mL of macrophage colony-stimulating factor (M-CSF; Miltenyi 130–096–485) was added to cRPMI for 6 days. Calu-3 and MDMs co-culture model preparation The cell culture model

(3) Timing-dependent anti-inflammatory effects of empagliflozin in monocyte-derived macrophages from post-myocardial infarct patients with type 2 diabetesCardiovascular diabetologyFebruary 12, 2026Cliff CL, Shah MU, Ward JK, Inghels M, Lee K, Squires PE, Hills CE.premium recombinant human Macrophage Colony Stimulating Factor (M-CSF; 20 ng/mL Miltenyi Biotec Cat# 130-096-492, CSF1_HUMAN, UniProtKB: P09603) to induce monocyte to macrophage differentiation. Cells were incubated

(4) A Subset of Pro‐inflammatory CXCL10+ LILRB2+ Macrophages Derives From Recipient Monocytes and Drives Renal Allograft RejectionAdvanced ScienceFebruary 1, 2026Alexis Varin, Jovanne Palvair, Lennie Messager, Jamal Bamoulid, Jasper Callemeyn, Mélanie Chaintreuil, Ludivine Dal Zuffo, Didier Ducloux, Imane Farhat, Mathieu Legendre, et al.macrophages, monocytes freshly isolated from healthy volunteers were subjected to M0 differentiation by M‐CSF (130‐096‐492, Miltenyi Biotec) at 25 ng/mL in X‐Vivo medium (BEBP02‐054Q, Lonza, Colmar, France) supplemented with

(5) A human pathophysiological 3D-bone marrow model reveals immune and stromal cell heterogeneityCommunications BiologyJanuary 3, 2026Mariette Giannini, Giulia Campione, Lea Torcq, Sylvain Donadelli, Maroua Bencheikh, Sophia Bounaud, Sandrine Jeanpierre, Kevin Geistlich, Veronique Maguer-Satta, Sylvain Lefort, et al.macrophage differentiation medium composed of X-VIVO-15 (Ozyme, BE02-060F), M-CSF (100 ng/mL, Miltenyi, 130-096-492), IL-3 (25 ng/mL, Miltenyi, 130-095-070), Glutamax (2 mM, Thermofisher, 35050061) and β-mercaptoethanol

(6) The chaperone GRP94 interacts with the proprotein convertase furin and regulates TGF-beta maturation in human primary M2 macrophagesCell Death DiscovDecember 15, 2025Valentin Baverel, Fangmin Wang, Carmen Garrido, Evelyne Kohlimonocytes were differentiated in M2 macrophages with 100 ng/mL macrophage colony stimulating factor (M-CSF, 130-096-492, Miltenyi, Bergisch Gladbach, Germany) in RPMI medium with 10% heat inactivated FBS during 6 days, with

(7) Chemically Defined, Efficient Megakaryocyte Production from Human Pluripotent Stem CellsCellsNovember 20, 2025Kim JE, Lee Y, Kim Y, Hwang SB, Choi YB, Han J, Jung J, Song JW, Joung JG, Ko JJ, et al.15750060 (Gibco)" Butyzamide,"Selleckchem(TX, USA)",S6988,HY-148748G (MedChemExpress) M-CSF,Miltenyi,130-096-492,170-076-172 Human albumin 20%,"SK Plasma(Andong, Gyeongsangbuk-do, Republic of Korea)",50800231,"GMP Human AB Serum

(8) Glycoprotein non-metastatic melanoma protein B is a potential biomarker for arthroplasty aseptic looseningScientific ReportsSeptember 12, 2025Patrik Schadzek, Alexander Derksen, Wiebke Behrens, Maike Kosanke, Oliver Dittrich-Breiholz, Anika Hamm, Kirsten Elger, Yvonne Roger, Ines Yang, Meike Stiesch, et al.100,000 U/L penicillin, 100 mg/L streptomycin, 25 ng/mL recombinant human M-CSF (Miltenyi Biotech #130-096-492, from E. coli)). Cells were incubated on ice for 10 min, then centrifuged at 300 x g for 5 min. The

(9) Plasmalogen remodeling modulates macrophage response to cytotoxic oxysterols and atherosclerotic plaque vulnerabilityCell Reports MedicineMay 20, 2025Antoine Jalil, Thomas Pilot, Thibaut Bourgeois, Aline Laubriet, Xiaoxu Li, Marc Diedisheim, Valérie Deckert, Charlène Magnani, Naig Le Guern, Jean-Paul Pais de Barros, et al.performed as previously described.18 In both cases, cells were treated during 5-7 days with human M-CSF (130-096-492, Miltenyi) until full macrophage differentiation. Liposome preparation Liposomes were prepared as

(10) Evolutionary conserved regulation of TFEB stability by the E3 ubiquitin ligase WWP2 modulates response to stress in vivoiScienceFebruary 21, 2025Juan A. Garcia-Sanchez, Estelle Bonnet, Céline Loubatier, Anne Doye, Guillaume Paillier, Fabien Segui, Frédéric Larbret, Paul Chaintreuil, Ludovic Batistic, Cédric Torre, et al.15140-122 Gentamycin,Gibco™,Cat # 15750045 L-glutamine 200mM,Gibco™,Cat # 25030 M-CSF,Miltenyi Biotech,Cat # 130-096-492 Cycloheximide,Sigma-Aldrich,Cat # 01810 MG132,Sigma-Aldrich,Cat # 474790 Protease and Phosphatase

(11) Macrophage caspase-8 inhibition accelerates necrotic core expansion in atheroma plaque in miceFrontiers in immunologyJanuary 1, 2025Pilot T, Solier S, Jalil A, Magnani C, Vanden Berghe T, Vandenabeele P, Masson D, Solary E, Thomas C.1x106 cells in 6-well plates. Differentiation into macrophages was induced by adding recombinant M-CSF (130-096-492, Miltenyi) for 5 to 6 days until complete differentiation. Plasma lipid analysis Plasma lipid parameters

(12) CENCAT enables immunometabolic profiling by measuring protein synthesis via bioorthogonal noncanonical amino acid taggingCell Reports MethodsOctober 21, 2024Frank Vrieling, Hendrik J.P. van der Zande, Britta Naus, Lisa Smeehuijzen, Julia I.P. van Heck, Bob J. Ignacio, Kimberly M. Bonger, Jan Van den Bossche, Sander Kersten, Rinke Stienstra,BD Biosciences,Cat#566385 Ficoll Paque Plus,Merck,Cat#GE17-1440-03 Recombinant Human M-CSF,Miltenyi Biotec,Cat#130-096-489 Recombinant Human GM-CSF,Miltenyi Biotec,Cat#130-093-864 Collagenase type II from Clostridium histolyticum,Merck,Cat#C6885 Collagenase

(13) Caspase-8 activation by cigarette smoke induces pro-inflammatory cell death of human macrophages exposed to lipopolysaccharideCell Death & DiseaseNovember 25, 2023Marta Cristaldi, Marco Buscetta, Maura Cimino, Agnese La Mensa, Maria Rita Giuffrè, Luigi Fiore, Claudia Carcione, Fabio Bucchieri, Francesca Rappa, Claudia Coronnello, et al.Saline w/o Calcium w/o Magnesium (ECB4004L) were purchased from Euroclone (Milan, Italy). Human M-CSF (130-096-493) was purchased from Miltenyi Biotec (Bergisch Gladbach, Germany). The following chemicals were obtained

(14) Quantifying spatial dynamics of Mycobacterium tuberculosis infection of human macrophages using microfabricated patternsCell Reports MethodsNovember 20, 2023Anca F. Savulescu, Nashied Peton, Delia Oosthuizen, Rudranil Hazra, Robert P. Rousseau, Musa M. Mhlanga, Anna K. Coussensserum,Sigma-Aldrich,Cat# H3667 Human GM-CSF,Miltenyi Biotec,Cat# 130-093-864 Human M-CSF,Miltenyi Biotec,Cat# 130-096-485 Recombinant Human Interferon gamma,R&D Systems,Cat# 285-IF-100 Accutase,Sigma-Aldrich,Cat# A6964 TRIzol reagent

(15) Multimodal cartography of human lymphopoiesis reveals B and T/NK/ILC lineages are subjected to differential regulationiScienceOctober 20, 2023Alhaj Hussen K, Chabaane E, Nelson E, Lekiashvili S, Diop S, Keita S, Evrard B, Lardenois A, Delord M, Verhoeyen E, et al."Human IL-15, premium grade",Miltenyi Biotech,Cat#130-095-765 "Human M-CSF, premium grade",Miltenyi Biotech,Cat#130-096-492 "Human SCF, premium grade",Miltenyi Biotech,Cat#130-096-695 "Human TPO, premium grade",Miltenyi Biotech,Cat#130-095-752 "Human IL-21, premium grade

(16) Label-free Chemical Characterization of Polarized Immune Cells in vitro and Host Response to Implanted Bio-instructive Polymers in vivo Using 3D OrbiSIMSBio ProtocAugust 5, 2023Waraporn Suvannapruk, Max K. Edney, Leanne E. Fisher, Jeni C. Luckett, Dong-Hyun Kim, David J. Scurr, Amir M. Ghaemmaghami, Morgan R. Alexandernumber: 130-093-919)Macrophage colony-stimulating factor (M-CSF) (Miltenyi Biotech, catalog number: 130-096-493)70% alcohol (any vendor)Optimal cutting temperature compound (OCT) (Agar Scientific, catalog number:

(17) Nanostructured Microparticles Repolarize Macrophages and Induce Cell Death in an In Vitro Model of Tumour-Associated MacrophagesPharmaceuticsJuly 5, 2023Salma Al-Fityan, Britta Diesel, Thorben Fischer, Emmanuel Ampofo, Annika Schomisch, Vida Mashayekhi, Marc Schneider, Alexandra K. Kiemermedium supplemented with 20 ng/mL human macrophage-colony stimulating factor (M-CSF, Miltenyi Biotec, 130-096-492) at 37 °C and 5% CO2 for 5 days. After differentiation, macrophages were left without polarization (M0)

(18) Ebola virus–like particles reprogram cellular metabolismJournal of Molecular MedicineMay 1, 2023Huaqi Tang, Yasmine Abouleila, Anno Saris, Yoshihiro Shimizu, Tom H. M. Ottenhoff, Alireza Mashaghifactor (GM-CSF; 130–093-864, Miltenyi Biotec) or 50 ng/ml macrophage colony-stimulating factor (M-CSF; 130–096-489, Miltenyi Biotec), respectively [10]. Cells were cultured at 37 °C/5% CO2 in RPMI 1640 medium (31,870,025,

(19) A druggable copper-signalling pathway that drives inflammationNatureMay 1, 2023Solier S, Müller S, Cañeque T, Versini A, Mansart A, Sindikubwabo F, Baron L, Emam L, Gestraud P, Pantoș GD, et al.macrophages, pan monocytes were treated with macrophage colony-stimulating factor (M-CSF, Miltenyi Biotec, 130-096-492, 100 ng ml−1) for 5 days and subsequently, IL-4 (Miltenyi Biotec, 130-093-921, 20 ng ml−1) was added

(20) DNA ultra-sensitive quantification, a technology for studying HIV unintegrated linear DNACell Reports MethodsApril 24, 2023Hélène Marie Roux, Suzanne Figueiredo, Lucas Sareoua, Maud Salmona, Juliette Hamroune, Lucie Adoux, Julie Migraine, Allan Hance, François Clavel, Rémi Cheynier, et al.Human IL-7 Premium grade,Miltenyi Biotec,Cat# 130-095-362 Human M-CSF Premium grade,Miltenyi Biotec,Cat# 130-096-489 Benzonase Nuclease,Merck Millipore,Cat# 70746 Dolutegravir,Selleckchem,Cat# S2667 Nevirapine,Selleckchem,Cat#

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2/12/2016Macrophage Differentiation Using M-CSF Max HübnerOur aim was to differentiate macrophages from human PBMCs using M-CSF in different concentrations.

Human M-CSF, premium grade from Miltenyi Biotec

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Human M-CSF, premium grade from Miltenyi Biotec

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