PURIFIED MOUSE NERVE GROWTH FACTOR 2.5S PMP04Z from Bio-Rad (Formerly AbD Serotec)

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PURIFIED MOUSE NERVE GROWTH FACTOR 2.5S

Published customer image:
Mouse Nerve Growth Factor 2.5S (PMP04Z) used in vitro to stimulate the differentiation of PC12 cells.
Image caption:
SB203580 increases lysosomal localization and degradation of αSyn and modulates lysosomal distribution and function.
A, NGF-differentiated PC12-αSyn/p25α cells were treated with 1 μM SB203580 and protease inhibitors E64 (10 μM) and LP (50/67 μg/ml, respectively) for 2 days before indirect immunofluorescence to reveal αSyn (LB509 mAb) and LAMP-1. Note increased localization of αSyn in LAMP1-positive compartments in SB203580-treated cells (arrows). The images are representative of two independent experiments. Bar represents 10 μm.
B, NGF-differentiated PC12-αSyn/p25α cells were cultured 2 days with or without 1 μM SB203580 before homogenization and fractionation of equal protein loads by sucrose gradient centrifugation. Collected fractions were analyzed by Western blotting for αSyn, cathepsin D, and LC3. Note the increased distribution of &apha;Syn and cathepsin D in heavy lysosomal fractions in SB203580-treated cells. The bands in the LC3 blot represent LC3-I (upper band) and LC3-II (lower band). The shown blots are representative of two independent experiments.br>
From: Borland H, Rasmussen I, Bjerregaard-Andersen K, Rasmussen M, Olsen A, Vilhardt F.
α-synuclein buildup is alleviated via
PURIFIED MOUSE NERVE GROWTH FACTOR 2.5S

Published customer image:
Mouse Nerve Growth Factor 2.5S (PMP04Z) used in vitro to stimulate the differentiation of PC12 cells.
Image caption:
SB203580 increases lysosomal localization and degradation of αSyn and modulates lysosomal distribution and function.
A, NGF-differentiated PC12-αSyn/p25α cells were treated with 1 μM SB203580 and protease inhibitors E64 (10 μM) and LP (50/67 μg/ml, respectively) for 2 days before indirect immunofluorescence to reveal αSyn (LB509 mAb) and LAMP-1. Note increased localization of αSyn in LAMP1-positive compartments in SB203580-treated cells (arrows). The images are representative of two independent experiments. Bar represents 10 μm.
B, NGF-differentiated PC12-αSyn/p25α cells were cultured 2 days with or without 1 μM SB203580 before homogenization and fractionation of equal protein loads by sucrose gradient centrifugation. Collected fractions were analyzed by Western blotting for αSyn, cathepsin D, and LC3. Note the increased distribution of &apha;Syn and cathepsin D in heavy lysosomal fractions in SB203580-treated cells. The bands in the LC3 blot represent LC3-I (upper band) and LC3-II (lower band). The shown blots are representative of two independent experiments.br>
From: Borland H, Rasmussen I, Bjerregaard-Andersen K, Rasmussen M, Olsen A, Vilhardt F.
α-synuclein buildup is alleviated via