Published customer image:
Recombinant Human interferon gamma (PHP050) used to treat human fibroblasts in vitro prior to infection with Toxoplasma
Image caption:
Lipidomic analyses on Δgra57, Δgra70, and Δgra71 using GC-MS-based approaches. (A) Lipidomics analysis comparing relative abundance of cholesterol over total fatty acids in WT and indicated knockout and complemented parasites grown in naive or IFNγ-stimulated HFFs. Indicated are means ± SEM (n = 2 to 4). P values were calculated using 2-way ANOVA with Sidak’s multiple-comparison test. (B) Fatty acid composition in Mol %. P values were calculated with a mixed-effects model with Sidak’s multiple-comparison test. n = 6 to 8 for WT and Δgra70, n = 2 for Δgra57, Δgra71, and Δgra70+GRA70. *, P <0.05; **, P <0.01; ***, P <0.001.

From: Krishnamurthy S, Maru P, Wang Y, Bitew MA, Mukhopadhyay D, Yamaryo-Botté Y, Paredes-Santos TC, Sangaré LO, Swale C, Botté CY, Saeij JPJ.
CRISPR Screens Identify Toxoplasma Genes That Determine Parasite Fitness in Interferon Gamma-Stimulated Human Cells.
mBio. 2023 Apr 25;14(2):e0006023.
doi: 10.1128/mbio.00060-23.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.